本文主要研究内容
作者盛守新（2019）在《乳酸菌胞外多糖佐剂提高金黄色葡萄球菌疫苗免疫效果的研究》一文中研究指出：金黄色葡萄球菌（Staphylococcus aureus,S.aureus）是一种人畜共患病原菌,可引起人和动物的多种感染。同时,S.aureus也是最常见的细菌性食物中毒病原之一。近些年,耐甲氧西林金黄色葡萄球菌广泛流行,目前S.aureus的感染面临“无药可治”的严峻挑战。因此,加强对S.aureus感染的免疫防治研究,研制高效、安全的新型金葡菌疫苗具有重要的经济意义和公共卫生学意义。多年来,全世界科研工作者对金葡菌疫苗研制进行了积极的探索,然而,目前尚无有效疫苗问世。许多学者认为,目前金葡菌疫苗的研究忽略了S.aureus对天然免疫系统的削弱和破坏作用,并进一步影响后续获得性免疫系统功能的发挥。采用有效的疫苗佐剂配伍,激发更强的免疫应答应该是金葡菌疫苗研制过程中要考虑的问题。然而,现有的免疫佐剂引起的免疫应答效应相对单一,极大的限制了疫苗免疫保护作用的充分发挥,因此研发有效、安全的新型免疫佐剂是未来金葡菌亚单位疫苗研发的重要发展方向。乳酸菌胞外多糖（Lactic acid bacteria exopolysaccharide,LAB EPS）是从乳酸菌发酵液中分离出来的活性成分。实验室前期研究证明EPS可以增强巨噬细胞免疫活性,促进树突状细胞成熟和前炎性因子分泌,并且可以显著提高小鼠对OVA抗原Th1型和Th17型免疫应答。本研究在实验室前期研究基础上,选取两种实验室分离的乳酸菌胞外多糖（EPS30和EPS29）配伍金黄色葡萄球菌3S疫苗(混合FhuD2、HlaH35L和EsxA-EsxB抗原)免疫小鼠,观察其在小鼠感染金黄色葡萄球菌菌血症模型和乳房炎感染模型中的免疫保护作用,为金黄色葡萄球菌疫苗佐剂的研究与开发提供新思路。本研究主要内容如下:1.分别制备金黄色葡萄球菌3S疫苗和乳酸菌EPS30及EPS29多糖,将EPS30和EPS29佐剂分别与3S疫苗混合后免疫小鼠。检测免疫小鼠抗原特异性IgG抗体水平、T淋巴细胞增殖能力以及脾脏T淋巴细胞的细胞因子表达情况。结果发现,EPS29和EPS30可以显著提高抗原特异性IgG抗体水平和T淋巴细胞增殖能力。同时,EPS29可以显著提高小鼠脾脏中CD4+IFN-γ+T细胞、CD4+IL-4+T细胞和CD4+IL-17+T细胞比例。2.通过尾静脉攻毒金黄色葡萄球菌,建立小鼠菌血症感染模型。观察各免疫组在感染金黄色葡萄球菌后的存活率、体重变化、各脏器细菌定植量及病理变化,对乳酸菌胞外多糖佐剂配伍3S疫苗在小鼠菌血症感染金葡菌模型的保护效果进行评价。结果发现,相比单独免疫金葡菌3S抗原,EPS29佐剂能有效提高小鼠感染金黄色葡萄球菌后的生存率,并能显著减少金葡菌在小鼠各脏器的定植,减轻小鼠各脏器病变。3.通过乳腺感染金黄色葡萄球菌,建立小鼠乳腺炎感染模型。检测免疫小鼠抗原特异性IgG抗体水平、观察小鼠乳腺炎临床症状、细菌在乳腺组织定植量、病理变化及炎性因子IL-6、TNF-α和IL-1β的表达水平,对乳酸菌胞外多糖佐剂配伍3S疫苗在小鼠乳腺感染金葡菌模型的保护效果进行评价。研究发现EPS29作为免疫佐剂,与3S抗原混合免疫小鼠,可以显著提高抗原特异性IgG抗体水平。与对照组相比,EPS29佐剂能显著减少小鼠乳腺内细菌的定植和减轻小鼠乳腺病变,降低乳腺中IL-6和TNF-α的含量。上述结果提示:乳酸菌胞外多糖佐剂EPS29配伍金黄色葡萄球菌3S疫苗可以有效的保护小鼠金黄色葡萄球菌全身感染和乳房感染,为新型有效的金黄色葡萄球菌疫苗佐剂的设计提供了实验基础与理论指导。
Abstract
jin huang se pu tao qiu jun （Staphylococcus aureus,S.aureus）shi yi chong ren chu gong huan bing yuan jun ,ke yin qi ren he dong wu de duo chong gan ran 。tong shi ,S.aureusye shi zui chang jian de xi jun xing shi wu zhong du bing yuan zhi yi 。jin xie nian ,nai jia yang xi lin jin huang se pu tao qiu jun an fan liu hang ,mu qian S.aureusde gan ran mian lin “mo yao ke zhi ”de yan jun tiao zhan 。yin ci ,jia jiang dui S.aureusgan ran de mian yi fang zhi yan jiu ,yan zhi gao xiao 、an quan de xin xing jin pu jun yi miao ju you chong yao de jing ji yi yi he gong gong wei sheng xue yi yi 。duo nian lai ,quan shi jie ke yan gong zuo zhe dui jin pu jun yi miao yan zhi jin hang le ji ji de tan suo ,ran er ,mu qian shang mo you xiao yi miao wen shi 。hu duo xue zhe ren wei ,mu qian jin pu jun yi miao de yan jiu hu lve le S.aureusdui tian ran mian yi ji tong de xiao ruo he po huai zuo yong ,bing jin yi bu ying xiang hou xu huo de xing mian yi ji tong gong neng de fa hui 。cai yong you xiao de yi miao zuo ji pei wu ,ji fa geng jiang de mian yi ying da ying gai shi jin pu jun yi miao yan zhi guo cheng zhong yao kao lv de wen ti 。ran er ,xian you de mian yi zuo ji yin qi de mian yi ying da xiao ying xiang dui chan yi ,ji da de xian zhi le yi miao mian yi bao hu zuo yong de chong fen fa hui ,yin ci yan fa you xiao 、an quan de xin xing mian yi zuo ji shi wei lai jin pu jun ya chan wei yi miao yan fa de chong yao fa zhan fang xiang 。ru suan jun bao wai duo tang （Lactic acid bacteria exopolysaccharide,LAB EPS）shi cong ru suan jun fa jiao ye zhong fen li chu lai de huo xing cheng fen 。shi yan shi qian ji yan jiu zheng ming EPSke yi zeng jiang ju shi xi bao mian yi huo xing ,cu jin shu tu zhuang xi bao cheng shou he qian yan xing yin zi fen bi ,bing ju ke yi xian zhe di gao xiao shu dui OVAkang yuan Th1xing he Th17xing mian yi ying da 。ben yan jiu zai shi yan shi qian ji yan jiu ji chu shang ,shua qu liang chong shi yan shi fen li de ru suan jun bao wai duo tang （EPS30he EPS29）pei wu jin huang se pu tao qiu jun 3Syi miao (hun ge FhuD2、HlaH35Lhe EsxA-EsxBkang yuan )mian yi xiao shu ,guan cha ji zai xiao shu gan ran jin huang se pu tao qiu jun jun xie zheng mo xing he ru fang yan gan ran mo xing zhong de mian yi bao hu zuo yong ,wei jin huang se pu tao qiu jun yi miao zuo ji de yan jiu yu kai fa di gong xin sai lu 。ben yan jiu zhu yao nei rong ru xia :1.fen bie zhi bei jin huang se pu tao qiu jun 3Syi miao he ru suan jun EPS30ji EPS29duo tang ,jiang EPS30he EPS29zuo ji fen bie yu 3Syi miao hun ge hou mian yi xiao shu 。jian ce mian yi xiao shu kang yuan te yi xing IgGkang ti shui ping 、Tlin ba xi bao zeng shi neng li yi ji pi zang Tlin ba xi bao de xi bao yin zi biao da qing kuang 。jie guo fa xian ,EPS29he EPS30ke yi xian zhe di gao kang yuan te yi xing IgGkang ti shui ping he Tlin ba xi bao zeng shi neng li 。tong shi ,EPS29ke yi xian zhe di gao xiao shu pi zang zhong CD4+IFN-γ+Txi bao 、CD4+IL-4+Txi bao he CD4+IL-17+Txi bao bi li 。2.tong guo wei jing mai gong du jin huang se pu tao qiu jun ,jian li xiao shu jun xie zheng gan ran mo xing 。guan cha ge mian yi zu zai gan ran jin huang se pu tao qiu jun hou de cun huo lv 、ti chong bian hua 、ge zang qi xi jun ding zhi liang ji bing li bian hua ,dui ru suan jun bao wai duo tang zuo ji pei wu 3Syi miao zai xiao shu jun xie zheng gan ran jin pu jun mo xing de bao hu xiao guo jin hang ping jia 。jie guo fa xian ,xiang bi chan du mian yi jin pu jun 3Skang yuan ,EPS29zuo ji neng you xiao di gao xiao shu gan ran jin huang se pu tao qiu jun hou de sheng cun lv ,bing neng xian zhe jian shao jin pu jun zai xiao shu ge zang qi de ding zhi ,jian qing xiao shu ge zang qi bing bian 。3.tong guo ru xian gan ran jin huang se pu tao qiu jun ,jian li xiao shu ru xian yan gan ran mo xing 。jian ce mian yi xiao shu kang yuan te yi xing IgGkang ti shui ping 、guan cha xiao shu ru xian yan lin chuang zheng zhuang 、xi jun zai ru xian zu zhi ding zhi liang 、bing li bian hua ji yan xing yin zi IL-6、TNF-αhe IL-1βde biao da shui ping ,dui ru suan jun bao wai duo tang zuo ji pei wu 3Syi miao zai xiao shu ru xian gan ran jin pu jun mo xing de bao hu xiao guo jin hang ping jia 。yan jiu fa xian EPS29zuo wei mian yi zuo ji ,yu 3Skang yuan hun ge mian yi xiao shu ,ke yi xian zhe di gao kang yuan te yi xing IgGkang ti shui ping 。yu dui zhao zu xiang bi ,EPS29zuo ji neng xian zhe jian shao xiao shu ru xian nei xi jun de ding zhi he jian qing xiao shu ru xian bing bian ,jiang di ru xian zhong IL-6he TNF-αde han liang 。shang shu jie guo di shi :ru suan jun bao wai duo tang zuo ji EPS29pei wu jin huang se pu tao qiu jun 3Syi miao ke yi you xiao de bao hu xiao shu jin huang se pu tao qiu jun quan shen gan ran he ru fang gan ran ,wei xin xing you xiao de jin huang se pu tao qiu jun yi miao zuo ji de she ji di gong le shi yan ji chu yu li lun zhi dao 。
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论文作者分别是来自内蒙古大学的盛守新，发表于刊物内蒙古大学2019-08-13论文，是一篇关于金黄色葡萄球菌疫苗论文,佐剂论文,乳酸菌胞外多糖论文,内蒙古大学2019-08-13论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自内蒙古大学2019-08-13论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。