本文主要研究内容
作者杜宇,童新宇,周丁丁,陈大福,熊翠玲,郑燕珍,徐国钧,王海朋,陈华枝,郭意龙,隆琦,郭睿（2019）在《中华蜜蜂幼虫肠道响应球囊菌胁迫的microRNA应答分析》一文中研究指出：【目的】蜜蜂球囊菌(Ascosphaera apis,简称球囊菌)是一种能够侵染中华蜜蜂(Apis cerana cerana,简称中蜂)幼虫的致死性真菌病原。微小RNA(microRNA,miRNA)可通过在转录后水平靶向抑制或降解mRNA而参与宿主与病原互作过程。本研究旨在对球囊菌胁迫的中蜂6日龄幼虫肠道的差异表达miRNA(DEmiRNA)及其靶基因进行深入分析,进而揭示DEmiRNA在中蜂响应球囊菌胁迫应答过程中的作用。【方法】利用Illumina MiSeq平台对正常及球囊菌胁迫的中蜂6日龄幼虫肠道(AcCK和AcT)进行测序,通过相关生物信息学软件预测DEmiRNA及其靶基因。通过Blast将靶基因注释到GO和KEGG数据库。利用Cytoscape软件构建DEmiRNA与其靶mRNA的调控网络。通过Stem-loop RT-PCR和qPCR验证测序数据的可靠性。【结果】本研究共预测出537个miRNA,其长度分布介于16–35 nt之间,且不同长度的miRNA首位碱基偏向性差异明显。通过Stem-loop RT-PCR证实了10个novel miRNA的表达。AcCK vs AcT比较组共有54个DEmiRNA,包含31个上调和23个下调miRNA,可分别靶向结合6170和8199个靶基因。GO分类结果显示上调和下调miRNA的靶基因分别涉及47和47个条目,富集基因数最多的皆为结合细胞进程和催化活性。KEGG代谢通路(pathway)富集分析结果表明上调和下调miRNA的靶基因分别富集在134和126条pathway,富集基因数最多的均为内吞作用和内质网中的蛋白质加工。调控网络分析结果表明,DEmiRNA及其靶mRNA形成十分复杂的调控关系;31个DEmiRNA可靶向结合51个与泛素介导的蛋白水解相关的mRNA,18个DEmiRNA可靶向结合14个与Jak-STAT信号通路相关的mRNA;miR-1277-x、miR-26-x、miR-27-y、miR-30-x、miR-6052-x等16个miRNA共同参与了上述两条免疫通路的调控。最后,随机挑选3个DEmiRNA进行qPCR验证,结果证明了测序数据的可靠性。【结论】本研究提供了中蜂幼虫肠道在球囊菌胁迫后期的miRNA的表达谱和差异表达信息,揭示了球囊菌与宿主之间在miRNA组学水平存在复杂的互作。miR-6052-x和miR-1277-x作为调控网络的核心可能通过影响细胞凋亡参与宿主的免疫防御,miR-26-x和miR-30-x可能通过调控Jak-STAT信号通路参与宿主的胁迫应答。本研究筛选出的关键DEmiRNA有望作为治疗白垩病的分子靶标。
Abstract
【mu de 】mi feng qiu nang jun (Ascosphaera apis,jian chen qiu nang jun )shi yi chong neng gou qin ran zhong hua mi feng (Apis cerana cerana,jian chen zhong feng )you chong de zhi si xing zhen jun bing yuan 。wei xiao RNA(microRNA,miRNA)ke tong guo zai zhuai lu hou shui ping ba xiang yi zhi huo jiang jie mRNAer can yu su zhu yu bing yuan hu zuo guo cheng 。ben yan jiu zhi zai dui qiu nang jun xie pai de zhong feng 6ri ling you chong chang dao de cha yi biao da miRNA(DEmiRNA)ji ji ba ji yin jin hang shen ru fen xi ,jin er jie shi DEmiRNAzai zhong feng xiang ying qiu nang jun xie pai ying da guo cheng zhong de zuo yong 。【fang fa 】li yong Illumina MiSeqping tai dui zheng chang ji qiu nang jun xie pai de zhong feng 6ri ling you chong chang dao (AcCKhe AcT)jin hang ce xu ,tong guo xiang guan sheng wu xin xi xue ruan jian yu ce DEmiRNAji ji ba ji yin 。tong guo Blastjiang ba ji yin zhu shi dao GOhe KEGGshu ju ku 。li yong Cytoscaperuan jian gou jian DEmiRNAyu ji ba mRNAde diao kong wang lao 。tong guo Stem-loop RT-PCRhe qPCRyan zheng ce xu shu ju de ke kao xing 。【jie guo 】ben yan jiu gong yu ce chu 537ge miRNA,ji chang du fen bu jie yu 16–35 ntzhi jian ,ju bu tong chang du de miRNAshou wei jian ji pian xiang xing cha yi ming xian 。tong guo Stem-loop RT-PCRzheng shi le 10ge novel miRNAde biao da 。AcCK vs AcTbi jiao zu gong you 54ge DEmiRNA,bao han 31ge shang diao he 23ge xia diao miRNA,ke fen bie ba xiang jie ge 6170he 8199ge ba ji yin 。GOfen lei jie guo xian shi shang diao he xia diao miRNAde ba ji yin fen bie she ji 47he 47ge tiao mu ,fu ji ji yin shu zui duo de jie wei jie ge xi bao jin cheng he cui hua huo xing 。KEGGdai xie tong lu (pathway)fu ji fen xi jie guo biao ming shang diao he xia diao miRNAde ba ji yin fen bie fu ji zai 134he 126tiao pathway,fu ji ji yin shu zui duo de jun wei nei tun zuo yong he nei zhi wang zhong de dan bai zhi jia gong 。diao kong wang lao fen xi jie guo biao ming ,DEmiRNAji ji ba mRNAxing cheng shi fen fu za de diao kong guan ji ;31ge DEmiRNAke ba xiang jie ge 51ge yu fan su jie dao de dan bai shui jie xiang guan de mRNA,18ge DEmiRNAke ba xiang jie ge 14ge yu Jak-STATxin hao tong lu xiang guan de mRNA;miR-1277-x、miR-26-x、miR-27-y、miR-30-x、miR-6052-xdeng 16ge miRNAgong tong can yu le shang shu liang tiao mian yi tong lu de diao kong 。zui hou ,sui ji tiao shua 3ge DEmiRNAjin hang qPCRyan zheng ,jie guo zheng ming le ce xu shu ju de ke kao xing 。【jie lun 】ben yan jiu di gong le zhong feng you chong chang dao zai qiu nang jun xie pai hou ji de miRNAde biao da pu he cha yi biao da xin xi ,jie shi le qiu nang jun yu su zhu zhi jian zai miRNAzu xue shui ping cun zai fu za de hu zuo 。miR-6052-xhe miR-1277-xzuo wei diao kong wang lao de he xin ke neng tong guo ying xiang xi bao diao wang can yu su zhu de mian yi fang yu ,miR-26-xhe miR-30-xke neng tong guo diao kong Jak-STATxin hao tong lu can yu su zhu de xie pai ying da 。ben yan jiu shai shua chu de guan jian DEmiRNAyou wang zuo wei zhi liao bai e bing de fen zi ba biao 。
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论文作者分别是来自微生物学报的杜宇,童新宇,周丁丁,陈大福,熊翠玲,郑燕珍,徐国钧,王海朋,陈华枝,郭意龙,隆琦,郭睿，发表于刊物微生物学报2019年09期论文，是一篇关于中华蜜蜂论文,幼虫论文,球囊菌论文,靶基因论文,调控网络论文,微生物学报2019年09期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自微生物学报2019年09期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。