:TaCML36, a wheat calmodulin-like protein,positively participates in an immune response to Rhizoctonia cerealis论文

:TaCML36, a wheat calmodulin-like protein,positively participates in an immune response to Rhizoctonia cerealis论文

本文主要研究内容

作者(2019)在《TaCML36, a wheat calmodulin-like protein,positively participates in an immune response to Rhizoctonia cerealis》一文中研究指出:Sharp eyespot, mainly caused by the soil-borne fungus Rhizoctonia cerealis, affects wheat(Triticum aestivum L.) production worldwide. In this study, we isolated Ta CML36 gene encoding a wheat calmodulin-like protein, and studied its defense role in protection against R. cerealis. Transcription of Ta CML36 was significantly elevated by both R. cerealis infection and exogenous ethylene treatment. Transcription was higher in resistant wheat lines than in susceptible ones. There were copies of Ta CML36 on chromosomes 5 A, 5 B, and 5 D. The Ta CML36 protein is composed of 183 amino acids and contains two calcium-binding EFhand domains. Subcellular localization assays in wheat indicated that Ta CML36 localizes in both the cytoplasm and nucleus. Virus-induced gene silencing and disease assessment indicated that compared to the controls, Ta CML36-silenced wheat plants displayed significantly reduced resistance to R. cerealis and had greater fungal biomass, suggesting that knockdown of Ta CML36 impaired host resistance. Knockdown of Ta CML36 also significantly repressed expression of pathogenesis-related genes such as Chitinase 1,PDF35, and PR17 C, the ethylene response factor-encoding gene Ta PIE1, and ethylene biosynthesis gene ACO2. Collectively, our results suggest that Ta CML36 positively participates in the innate immune response to R. cerealis infection by modulating expression of defense-associated genes possibly in the ethylene signaling pathway.

Abstract

Sharp eyespot, mainly caused by the soil-borne fungus Rhizoctonia cerealis, affects wheat(Triticum aestivum L.) production worldwide. In this study, we isolated Ta CML36 gene encoding a wheat calmodulin-like protein, and studied its defense role in protection against R. cerealis. Transcription of Ta CML36 was significantly elevated by both R. cerealis infection and exogenous ethylene treatment. Transcription was higher in resistant wheat lines than in susceptible ones. There were copies of Ta CML36 on chromosomes 5 A, 5 B, and 5 D. The Ta CML36 protein is composed of 183 amino acids and contains two calcium-binding EFhand domains. Subcellular localization assays in wheat indicated that Ta CML36 localizes in both the cytoplasm and nucleus. Virus-induced gene silencing and disease assessment indicated that compared to the controls, Ta CML36-silenced wheat plants displayed significantly reduced resistance to R. cerealis and had greater fungal biomass, suggesting that knockdown of Ta CML36 impaired host resistance. Knockdown of Ta CML36 also significantly repressed expression of pathogenesis-related genes such as Chitinase 1,PDF35, and PR17 C, the ethylene response factor-encoding gene Ta PIE1, and ethylene biosynthesis gene ACO2. Collectively, our results suggest that Ta CML36 positively participates in the innate immune response to R. cerealis infection by modulating expression of defense-associated genes possibly in the ethylene signaling pathway.

论文参考文献

  • [1].Winter wheat identification by integrating spectral and temporal information derived from multi-resolution remote sensing data[J]. ZHANG Xi-wang,LIU Jian-feng,Zhenyue Qin,QIN Fen.  Journal of Integrative Agriculture.2019(11)
  • [2].Yield Gap Analysis of Wheat in Rice-wheat Rotation Regions of Anhui Province,China[J]. Xianfang HE,Li ZHAO,Ze LIU,Muhammad SAJJAD,Jianlai WANG.  Asian Agricultural Research.2019(11)
  • [3].Breeding new cultivars for sustainable wheat production[J]. Hongjie Li,Timothy D.Murray,Robert A.McIntosh,Yang Zhou.  The Crop Journal.2019(06)
  • [4].Diversity and sub-functionalization of TaGW8 homoeologs hold potential for genetic yield improvement in wheat[J]. Lin Ma,Chenyang Hao,Hongxia Liu,Jian Hou,Tian Li,Xueyong Zhang.  The Crop Journal.2019(06)
  • [5].Integrated transcriptomics and metabolomics analyses provide insights into cold stress response in wheat[J]. Yong Zhao,Meng Zhou,Ke Xu,Jiahao Li,Shanshan Li,Shuhua Zhang,Xueju Yang.  The Crop Journal.2019(06)
  • [6].A brief history of wheat utilization in China[J]. Minxia LU,Liang CHEN,Jinxiu WANG,Ruiliang LIU,Yang YANG,Meng WEI,Guanghui DONG.  Frontiers of Agricultural Science and Engineering.2019(03)
  • [7].Genetic improvement of heat tolerance in wheat:Recent progress in understanding the underlying molecular mechanisms[J]. Zhongfu Ni,Hongjian Li,Yue Zhao,Huiru Peng,Zhaorong Hu,Mingming Xin,Qixin Sun.  The Crop Journal.2018(01)
  • [8].Evaluation of a new method for quantification of heat tolerance in different wheat cultivars[J]. LI Qiang,WANG Zheng-rui,LI Ding,WEI Jian-wei,QIAO Wen-chen,MENG Xiang-hai,SUN Shu-luan,LI Hui-min,ZHAO Ming-hui,CHEN Xiu-min,ZHAO Feng-wu.  Journal of Integrative Agriculture.2018(04)
  • [9].Spatiotemporal differentiation of changes in wheat phenology in China under climate change from 1981 to 2010[J]. Yujie LIU,Qiaomin CHEN,Quansheng GE,Junhu DAI.  Science China(Earth Sciences).2018(08)
  • [10].Alternate row mulching optimizes soil temperature and water conditions and improves wheat yield in dryland farming[J]. YAN Qiu-yan,DONG Fei,LOU Ge,YANG Feng,LU Jin-xiu,LI Feng,ZHANG Jian-cheng,LI Jun-hui,DUAN Zeng-qiang.  Journal of Integrative Agriculture.2018(11)
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