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作者（2019）在《Effect of H2O2 on Growth of Collard（ Brassis oleracea L. ） Seedlings Under Salt Stress》一文中研究指出：Collard variety（ Brassis oleracea L. var. acephala f. tricolor Hort.） as a research material was treated with exogenous H2O2 and H2O2 scavenger dimethyl thiourea under 100 mmol/L NaC l stress. Two days later,growth rate,dry weight,fresh weight and relative water content of the plants were determined. After 6h of treatment,the activity and gene expression of three antioxidant enzymes,superoxide dismutase（ SOD）,catalase（ CAT） and ascorbate peroxidase（ APX） in plants,were measured. The results showed that the growth rate,dry weight,fresh weight,relative water content,and the activity and gene expression of the three antioxidant enzymes in collard seedlings were higher in the treatment of salt stress with the addition of 0. 05 mmol/L exogenous H2O2 than in the simple salt stress treatment; and when endogenous H2O2 was removed,the growth rate,dry weight,fresh weight,relative water content,and the activity and gene expression of the three antioxidant enzymes in plant seedlings were lower than those under simple salt stress. It is speculated that under salt stress,H2O2 is involved in the regulation of antioxidant defense gene expression,and it might be an important regulator of salt-induced antioxidant system in collard leaves.
Abstract
Collard variety（ Brassis oleracea L. var. acephala f. tricolor Hort.） as a research material was treated with exogenous H2O2 and H2O2 scavenger dimethyl thiourea under 100 mmol/L NaC l stress. Two days later,growth rate,dry weight,fresh weight and relative water content of the plants were determined. After 6h of treatment,the activity and gene expression of three antioxidant enzymes,superoxide dismutase（ SOD）,catalase（ CAT） and ascorbate peroxidase（ APX） in plants,were measured. The results showed that the growth rate,dry weight,fresh weight,relative water content,and the activity and gene expression of the three antioxidant enzymes in collard seedlings were higher in the treatment of salt stress with the addition of 0. 05 mmol/L exogenous H2O2 than in the simple salt stress treatment; and when endogenous H2O2 was removed,the growth rate,dry weight,fresh weight,relative water content,and the activity and gene expression of the three antioxidant enzymes in plant seedlings were lower than those under simple salt stress. It is speculated that under salt stress,H2O2 is involved in the regulation of antioxidant defense gene expression,and it might be an important regulator of salt-induced antioxidant system in collard leaves.
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