本文主要研究内容
作者刘春晓（2019）在《拟南芥中AtR8 lncRNA功能及作用机制初探》一文中研究指出：非编码RNA(non-coding RNA)不翻译成蛋白质,它们通过转录、转录后及翻译水平调控靶基因表达,在生物体生长发育过程中发挥重要作用。大于200个核苷酸长度的为长非编码RNA(long non-coding RNA,IncRNA),它们以调节因子形式,在植物开花、雄性不育、营养代谢、生物和非生物胁迫等过程中起着重要作用。AtR8lncRNA(259 nt)是 2012 年于拟南芥(Arabidopsisthaliana)中发现的一个 RNA 聚合酶IⅢ转录的lncRNA,种子萌发期其于拟南芥根尖端大量表达,并响应ABA、NaCl、GA3、SA、Mannitol等应激环境;其同源物存在于结球甘蓝(Brassica oleracea L.var capitat L.)和白菜(Brassica rapa ar pekinensis)等十字花科芸薹属植物中。目前,AtR8 lncRNA的详细功能及作用机制尚不清楚。本研究表明,正常条件下,atr8 突变体导致种子干瘪、长角果发育不完全、莲座叶边缘锯齿状、成熟植株纤细矮小、种子萌发率大幅度降低,这些表型在AtR8 lncRNA过表达(AtR8-AtOX)植株中没有被观察到。ABA应激条件下,atr8突变体和AtR8-AtOX均抑制了种子萌发。分子水平分析表明,正常萌发和ABA应激处理下,atr8突变体和AtR8-AtOX在萌发期间均影响了 ABA信号径路中萌发相关重要基因表达。本研究也深入分析了结球甘蓝中AtR8 lncRNA的同源物BoNR8 lncRNA(272nt)的功能及作用机制。我们研究结果表明,BoNR8 lncRNA主要于结球甘蓝萌发种子根伸长区表皮层大量表达,响应ABA和NaCl应激。正常条件下,拟南芥中BoNR8 lncRNA过表达主要通过影响AtRAV1基因表达抑制了种子萌发、角果形成、根生长。ABA应激下,BoNR8 lncRNA主要通过影响ABA信号径路中WRKY6、AARV1、RD29、SnRK2.3等萌发相关重要基因表达降低了萌发种子对ABA的敏感。
Abstract
fei bian ma RNA(non-coding RNA)bu fan yi cheng dan bai zhi ,ta men tong guo zhuai lu 、zhuai lu hou ji fan yi shui ping diao kong ba ji yin biao da ,zai sheng wu ti sheng chang fa yo guo cheng zhong fa hui chong yao zuo yong 。da yu 200ge he gan suan chang du de wei chang fei bian ma RNA(long non-coding RNA,IncRNA),ta men yi diao jie yin zi xing shi ,zai zhi wu kai hua 、xiong xing bu yo 、ying yang dai xie 、sheng wu he fei sheng wu xie pai deng guo cheng zhong qi zhao chong yao zuo yong 。AtR8lncRNA(259 nt)shi 2012 nian yu ni na gai (Arabidopsisthaliana)zhong fa xian de yi ge RNA ju ge mei IⅢzhuai lu de lncRNA,chong zi meng fa ji ji yu ni na gai gen jian duan da liang biao da ,bing xiang ying ABA、NaCl、GA3、SA、Mannitoldeng ying ji huan jing ;ji tong yuan wu cun zai yu jie qiu gan lan (Brassica oleracea L.var capitat L.)he bai cai (Brassica rapa ar pekinensis)deng shi zi hua ke yun tai shu zhi wu zhong 。mu qian ,AtR8 lncRNAde xiang xi gong neng ji zuo yong ji zhi shang bu qing chu 。ben yan jiu biao ming ,zheng chang tiao jian xia ,atr8 tu bian ti dao zhi chong zi gan bie 、chang jiao guo fa yo bu wan quan 、lian zuo xie bian yuan ju chi zhuang 、cheng shou zhi zhu qian xi ai xiao 、chong zi meng fa lv da fu du jiang di ,zhe xie biao xing zai AtR8 lncRNAguo biao da (AtR8-AtOX)zhi zhu zhong mei you bei guan cha dao 。ABAying ji tiao jian xia ,atr8tu bian ti he AtR8-AtOXjun yi zhi le chong zi meng fa 。fen zi shui ping fen xi biao ming ,zheng chang meng fa he ABAying ji chu li xia ,atr8tu bian ti he AtR8-AtOXzai meng fa ji jian jun ying xiang le ABAxin hao jing lu zhong meng fa xiang guan chong yao ji yin biao da 。ben yan jiu ye shen ru fen xi le jie qiu gan lan zhong AtR8 lncRNAde tong yuan wu BoNR8 lncRNA(272nt)de gong neng ji zuo yong ji zhi 。wo men yan jiu jie guo biao ming ,BoNR8 lncRNAzhu yao yu jie qiu gan lan meng fa chong zi gen shen chang ou biao pi ceng da liang biao da ,xiang ying ABAhe NaClying ji 。zheng chang tiao jian xia ,ni na gai zhong BoNR8 lncRNAguo biao da zhu yao tong guo ying xiang AtRAV1ji yin biao da yi zhi le chong zi meng fa 、jiao guo xing cheng 、gen sheng chang 。ABAying ji xia ,BoNR8 lncRNAzhu yao tong guo ying xiang ABAxin hao jing lu zhong WRKY6、AARV1、RD29、SnRK2.3deng meng fa xiang guan chong yao ji yin biao da jiang di le meng fa chong zi dui ABAde min gan 。
论文参考文献
论文详细介绍
论文作者分别是来自东北林业大学的刘春晓，发表于刊物东北林业大学2019-11-18论文，是一篇关于种子萌发论文,聚合酶论文,东北林业大学2019-11-18论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自东北林业大学2019-11-18论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：种子萌发论文; 聚合酶论文; 东北林业大学2019-11-18论文;