本文主要研究内容
作者王晓燕,季仁东,陈仁文（2019）在《异丙甲草胺农药残留吸收光谱检测方法研究》一文中研究指出：除草剂可以快速、有效地进行除草,已被广泛应用,但是在除草剂使用同时也会对周围环境和农作物带来一定程度的污染,例如农业生产过程中经常发现由于除草剂使用不当而使果树中毒的现象。异丙甲草胺是一种酰胺类选择性除草剂,被广泛应用于旱地作物、蔬菜和果园、苗圃。根据相关文献报道,基于气相色谱法、气相色谱-质谱联用法和固相萃取等方法可以实现异丙甲草胺残留检测,而基于吸收光谱法对异丙甲草胺的分析未见相关文献报道,提出直接利用吸收光谱及其导数光谱分析法实现异丙甲草胺农药及其在苹果汁中的农药残留检测。首先利用分光光度计对不同浓度异丙甲草胺药液进行吸收光谱实验研究,发现在266nm处有明显吸收光谱特征峰。对农药吸收光谱进行拟合分析,得到异丙甲草胺药液浓度和吸光度之间预测模型函数方程,函数方程为y=2.147 09x+0.031 98,相关系数为0.998 5。然后利用分光光度计对苹果汁-异丙甲草胺混合溶液进行吸收光谱实验研究,相对于纯苹果汁吸收光谱,在混合溶液吸收光谱中发现266nm处为异丙甲草胺所对应的特征峰。对苹果汁中药物浓度和吸光度进行建模,模型函数为:y=0.704 9+0.826 8x,其相关系数为0.991 1。可以看出,当苹果汁中异丙甲草胺残留量很低时,其农药吸收光谱特征峰并不明显。为进一步提高检测效果,对混合溶液吸收光谱进行一阶导数处理,得到其一阶导数吸收光谱。与苹果汁吸收导数光谱相比较,苹果汁-农药混合溶液导数光谱有两个明显特征光谱峰,分别位于269和276nm处。进一步分析苹果汁-异丙甲草胺混合溶液的导数吸收光谱峰值与农药含量之间的关系,对异丙甲草胺含量与导数光谱吸光度进行函数拟合。其中269nm对应预测模型函数关系式:y=0.005 3-0.090 6x,相关系数r=0.992 5;276nm对应预测模型函数关系式为y=-0.000 769-0.302 8x,相关系数r=0.990 6。为验证由吸收光谱和其一阶导数光谱所得苹果汁中农药残留预测模型的准确性,另外配置五种浓度苹果汁-异丙甲草胺混合溶液。然后在同等条件下对其进行吸收光谱实验,将266,269和276nm处的吸光度分别代入对应模型函数可求得浓度预测值,结合已知浓度值可计算其平均回收率,其中吸收光谱266nm对应平均回收率为104.68%,导数光谱269nm对应平均回收率为104.59%,276nm对应平均回收率为105.18%。对苹果汁中异丙甲草胺检测模型进行分析,计算得到检出限（LOD）和定量限（LOQ）参数值,其中原始吸收光谱对应LOD和LOQ分别为0.014 8和0.049 2mg·mL-1,一阶导数光谱对应LOD和LOQ最小值分别为0.001 5和0.004 9mg·mL-1。研究结果表明,采用吸收光谱方法对苹果汁中异丙甲草胺进行直接检测与分析是快速和可行有效的,而且对吸收光谱进行导数运算处理后,检测效果更优。
Abstract
chu cao ji ke yi kuai su 、you xiao de jin hang chu cao ,yi bei an fan ying yong ,dan shi zai chu cao ji shi yong tong shi ye hui dui zhou wei huan jing he nong zuo wu dai lai yi ding cheng du de wu ran ,li ru nong ye sheng chan guo cheng zhong jing chang fa xian you yu chu cao ji shi yong bu dang er shi guo shu zhong du de xian xiang 。yi bing jia cao an shi yi chong xian an lei shua ze xing chu cao ji ,bei an fan ying yong yu han de zuo wu 、shu cai he guo yuan 、miao pu 。gen ju xiang guan wen suo bao dao ,ji yu qi xiang se pu fa 、qi xiang se pu -zhi pu lian yong fa he gu xiang cui qu deng fang fa ke yi shi xian yi bing jia cao an can liu jian ce ,er ji yu xi shou guang pu fa dui yi bing jia cao an de fen xi wei jian xiang guan wen suo bao dao ,di chu zhi jie li yong xi shou guang pu ji ji dao shu guang pu fen xi fa shi xian yi bing jia cao an nong yao ji ji zai ping guo zhi zhong de nong yao can liu jian ce 。shou xian li yong fen guang guang du ji dui bu tong nong du yi bing jia cao an yao ye jin hang xi shou guang pu shi yan yan jiu ,fa xian zai 266nmchu you ming xian xi shou guang pu te zheng feng 。dui nong yao xi shou guang pu jin hang ni ge fen xi ,de dao yi bing jia cao an yao ye nong du he xi guang du zhi jian yu ce mo xing han shu fang cheng ,han shu fang cheng wei y=2.147 09x+0.031 98,xiang guan ji shu wei 0.998 5。ran hou li yong fen guang guang du ji dui ping guo zhi -yi bing jia cao an hun ge rong ye jin hang xi shou guang pu shi yan yan jiu ,xiang dui yu chun ping guo zhi xi shou guang pu ,zai hun ge rong ye xi shou guang pu zhong fa xian 266nmchu wei yi bing jia cao an suo dui ying de te zheng feng 。dui ping guo zhi zhong yao wu nong du he xi guang du jin hang jian mo ,mo xing han shu wei :y=0.704 9+0.826 8x,ji xiang guan ji shu wei 0.991 1。ke yi kan chu ,dang ping guo zhi zhong yi bing jia cao an can liu liang hen di shi ,ji nong yao xi shou guang pu te zheng feng bing bu ming xian 。wei jin yi bu di gao jian ce xiao guo ,dui hun ge rong ye xi shou guang pu jin hang yi jie dao shu chu li ,de dao ji yi jie dao shu xi shou guang pu 。yu ping guo zhi xi shou dao shu guang pu xiang bi jiao ,ping guo zhi -nong yao hun ge rong ye dao shu guang pu you liang ge ming xian te zheng guang pu feng ,fen bie wei yu 269he 276nmchu 。jin yi bu fen xi ping guo zhi -yi bing jia cao an hun ge rong ye de dao shu xi shou guang pu feng zhi yu nong yao han liang zhi jian de guan ji ,dui yi bing jia cao an han liang yu dao shu guang pu xi guang du jin hang han shu ni ge 。ji zhong 269nmdui ying yu ce mo xing han shu guan ji shi :y=0.005 3-0.090 6x,xiang guan ji shu r=0.992 5;276nmdui ying yu ce mo xing han shu guan ji shi wei y=-0.000 769-0.302 8x,xiang guan ji shu r=0.990 6。wei yan zheng you xi shou guang pu he ji yi jie dao shu guang pu suo de ping guo zhi zhong nong yao can liu yu ce mo xing de zhun que xing ,ling wai pei zhi wu chong nong du ping guo zhi -yi bing jia cao an hun ge rong ye 。ran hou zai tong deng tiao jian xia dui ji jin hang xi shou guang pu shi yan ,jiang 266,269he 276nmchu de xi guang du fen bie dai ru dui ying mo xing han shu ke qiu de nong du yu ce zhi ,jie ge yi zhi nong du zhi ke ji suan ji ping jun hui shou lv ,ji zhong xi shou guang pu 266nmdui ying ping jun hui shou lv wei 104.68%,dao shu guang pu 269nmdui ying ping jun hui shou lv wei 104.59%,276nmdui ying ping jun hui shou lv wei 105.18%。dui ping guo zhi zhong yi bing jia cao an jian ce mo xing jin hang fen xi ,ji suan de dao jian chu xian （LOD）he ding liang xian （LOQ）can shu zhi ,ji zhong yuan shi xi shou guang pu dui ying LODhe LOQfen bie wei 0.014 8he 0.049 2mg·mL-1,yi jie dao shu guang pu dui ying LODhe LOQzui xiao zhi fen bie wei 0.001 5he 0.004 9mg·mL-1。yan jiu jie guo biao ming ,cai yong xi shou guang pu fang fa dui ping guo zhi zhong yi bing jia cao an jin hang zhi jie jian ce yu fen xi shi kuai su he ke hang you xiao de ,er ju dui xi shou guang pu jin hang dao shu yun suan chu li hou ,jian ce xiao guo geng you 。
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论文详细介绍
论文作者分别是来自光谱学与光谱分析的王晓燕,季仁东,陈仁文，发表于刊物光谱学与光谱分析2019年01期论文，是一篇关于农药残留论文,异丙甲草胺论文,吸收光谱论文,导数光谱论文,光谱学与光谱分析2019年01期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自光谱学与光谱分析2019年01期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
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