本文主要研究内容
作者江贝（2019）在《龙血素B对脂肪细胞脂质合成的影响》一文中研究指出：脂质代谢是体内重要且复杂的生化反应,指生物体内脂质在各种相关酶的催化下,消化吸收、合成与分解的过程,加工成机体所需要的物质,确保正常生理机能的运作,对于生命活动具有重要意义。脂质沉积是脂质代谢中重要的一部分,涉及到诸多领域,包括畜牧行业家禽家畜的肉质。近年来,天然产物参与成脂调控的研究逐渐成为研究的热点,因此本研究以3T3-L1脂肪细胞为研究对象,通过添加天然小分子龙血素B(Loureirin B),检测其对脂肪细胞的成脂情况及其影响成脂的机制,进而探究其对脂质代谢的影响,从而为调控猪的脂肪沉积提供理论依据。本研究主要获得以下结果:1、在3T3-L1脂肪细胞增殖期加入80μmol/L的Loureirin B处理24h,CCK-8试剂盒检测细胞活力,结果表明80μmol/L的Loureirin B对3T3-L1脂肪细胞的活力无显著影响;2、龙血素B(80μmol/L)对3T3-L1脂肪细胞的增殖有抑制作用。在3T3-L1脂肪细胞增殖期加入80μmol/L的Loureirin B处理48h-72h,利用EdU染色可发现Loureirin B处理可显著降低细胞的数目(P<0.01);RT-PCR检测增殖相关基因的mRNA表达,Western blot检测相关蛋白的表达水平,结果发现Loureirin B处理可显著下调促增殖基因CyclinD、CyclinE的mRNA和蛋白的表达(P<0.01),上调抑增殖基因的P53 mRNA水平(P<0.05),与增殖有关的ERK信号通路中ERK1/2的磷酸化水平下降(P<0.05);流式细胞术检测细胞增殖周期,结果发现Loureirin B处理后,使S期的细胞数目减少,可使细胞的增殖周期阻滞在G2期。3、龙血素B(80μmol/L)显著促进3T3-L1脂肪细胞的成脂与分化。在3T3-L1脂肪细胞的分化期加入80μmol/L的Loureirin B,油红O染色发现与对照组相比Loureirin B能显著促进3T3-L1脂肪细胞内脂滴的合成;RT-PCR和Western blot检测相关基因和蛋白表达水平。结果发现,Loureirin B处理后在mRNA和蛋白水平上,脂合成和脂分解相关基因和蛋白表达量均上升,但脂质合成相关基因的mRNA和蛋白的表达水平上调幅度较高。为了进一步探究Loureirin B促进成脂的机制,我们首先确定了其可能通过GPR120来发挥作用,通过RT-PCR和Western blot结果发现处理组GPR120的mRNA水平和蛋白表达都显著上升(P<0.01),加入GPR120的抑制剂AH7614后,细胞内的脂滴明显减少,通过RT-PCR和Western blot得到的结果与表型一致;进一步研究发现,胰岛素信号通路的敏感性增强,促进了GLUT4的转位,进而促进葡萄糖的吸收,最终促进细胞内脂质的合成。综上所述,80μmol/L的天然小分子龙血素B可显著抑制脂肪细胞的增殖,在分化期可显著促进细胞内脂质的沉积,是一种潜在的促进动物体内脂肪沉积、改善肉品质的候选天然小分子。
Abstract
zhi zhi dai xie shi ti nei chong yao ju fu za de sheng hua fan ying ,zhi sheng wu ti nei zhi zhi zai ge chong xiang guan mei de cui hua xia ,xiao hua xi shou 、ge cheng yu fen jie de guo cheng ,jia gong cheng ji ti suo xu yao de wu zhi ,que bao zheng chang sheng li ji neng de yun zuo ,dui yu sheng ming huo dong ju you chong yao yi yi 。zhi zhi chen ji shi zhi zhi dai xie zhong chong yao de yi bu fen ,she ji dao zhu duo ling yu ,bao gua chu mu hang ye jia qin jia chu de rou zhi 。jin nian lai ,tian ran chan wu can yu cheng zhi diao kong de yan jiu zhu jian cheng wei yan jiu de re dian ,yin ci ben yan jiu yi 3T3-L1zhi fang xi bao wei yan jiu dui xiang ,tong guo tian jia tian ran xiao fen zi long xie su B(Loureirin B),jian ce ji dui zhi fang xi bao de cheng zhi qing kuang ji ji ying xiang cheng zhi de ji zhi ,jin er tan jiu ji dui zhi zhi dai xie de ying xiang ,cong er wei diao kong zhu de zhi fang chen ji di gong li lun yi ju 。ben yan jiu zhu yao huo de yi xia jie guo :1、zai 3T3-L1zhi fang xi bao zeng shi ji jia ru 80μmol/Lde Loureirin Bchu li 24h,CCK-8shi ji he jian ce xi bao huo li ,jie guo biao ming 80μmol/Lde Loureirin Bdui 3T3-L1zhi fang xi bao de huo li mo xian zhe ying xiang ;2、long xie su B(80μmol/L)dui 3T3-L1zhi fang xi bao de zeng shi you yi zhi zuo yong 。zai 3T3-L1zhi fang xi bao zeng shi ji jia ru 80μmol/Lde Loureirin Bchu li 48h-72h,li yong EdUran se ke fa xian Loureirin Bchu li ke xian zhe jiang di xi bao de shu mu (P<0.01);RT-PCRjian ce zeng shi xiang guan ji yin de mRNAbiao da ,Western blotjian ce xiang guan dan bai de biao da shui ping ,jie guo fa xian Loureirin Bchu li ke xian zhe xia diao cu zeng shi ji yin CyclinD、CyclinEde mRNAhe dan bai de biao da (P<0.01),shang diao yi zeng shi ji yin de P53 mRNAshui ping (P<0.05),yu zeng shi you guan de ERKxin hao tong lu zhong ERK1/2de lin suan hua shui ping xia jiang (P<0.05);liu shi xi bao shu jian ce xi bao zeng shi zhou ji ,jie guo fa xian Loureirin Bchu li hou ,shi Sji de xi bao shu mu jian shao ,ke shi xi bao de zeng shi zhou ji zu zhi zai G2ji 。3、long xie su B(80μmol/L)xian zhe cu jin 3T3-L1zhi fang xi bao de cheng zhi yu fen hua 。zai 3T3-L1zhi fang xi bao de fen hua ji jia ru 80μmol/Lde Loureirin B,you gong Oran se fa xian yu dui zhao zu xiang bi Loureirin Bneng xian zhe cu jin 3T3-L1zhi fang xi bao nei zhi di de ge cheng ;RT-PCRhe Western blotjian ce xiang guan ji yin he dan bai biao da shui ping 。jie guo fa xian ,Loureirin Bchu li hou zai mRNAhe dan bai shui ping shang ,zhi ge cheng he zhi fen jie xiang guan ji yin he dan bai biao da liang jun shang sheng ,dan zhi zhi ge cheng xiang guan ji yin de mRNAhe dan bai de biao da shui ping shang diao fu du jiao gao 。wei le jin yi bu tan jiu Loureirin Bcu jin cheng zhi de ji zhi ,wo men shou xian que ding le ji ke neng tong guo GPR120lai fa hui zuo yong ,tong guo RT-PCRhe Western blotjie guo fa xian chu li zu GPR120de mRNAshui ping he dan bai biao da dou xian zhe shang sheng (P<0.01),jia ru GPR120de yi zhi ji AH7614hou ,xi bao nei de zhi di ming xian jian shao ,tong guo RT-PCRhe Western blotde dao de jie guo yu biao xing yi zhi ;jin yi bu yan jiu fa xian ,yi dao su xin hao tong lu de min gan xing zeng jiang ,cu jin le GLUT4de zhuai wei ,jin er cu jin pu tao tang de xi shou ,zui zhong cu jin xi bao nei zhi zhi de ge cheng 。zeng shang suo shu ,80μmol/Lde tian ran xiao fen zi long xie su Bke xian zhe yi zhi zhi fang xi bao de zeng shi ,zai fen hua ji ke xian zhe cu jin xi bao nei zhi zhi de chen ji ,shi yi chong qian zai de cu jin dong wu ti nei zhi fang chen ji 、gai shan rou pin zhi de hou shua tian ran xiao fen zi 。
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论文作者分别是来自西北农林科技大学的江贝，发表于刊物西北农林科技大学2019-07-11论文，是一篇关于龙血素论文,脂肪细胞论文,细胞增殖论文,细胞分化论文,西北农林科技大学2019-07-11论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自西北农林科技大学2019-07-11论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：龙血素论文; 脂肪细胞论文; 细胞增殖论文; 细胞分化论文; 西北农林科技大学2019-07-11论文;