本文主要研究内容
作者（2019）在《MicroRNAs transcriptionally regulate promoter activity in Arabidopsis thaliana》一文中研究指出：Micro RNAs(mi RNAs) are vital regulators that repress gene expression in the cytoplasm in two main ways: m RNA degradation and translational inhibition. Several animal studies have shown that mi RNAs also target promoters, thereby activating expression.Whether this mi RNA action also occurs in plants is unknown. In this study, we demonstrated that several mi RNAs regulate target promoters in Arabidopsis thaliana. For example, mi R5658 was predominantly present in the nucleus and activated the expression of AT3 G25290 directly by binding to its promoter. Our observations suggest that this mode of action may be a general feature of plant mi RNAs, and thus provide insight into the vital roles of plant mi RNAs in the nucleus.
Abstract
Micro RNAs(mi RNAs) are vital regulators that repress gene expression in the cytoplasm in two main ways: m RNA degradation and translational inhibition. Several animal studies have shown that mi RNAs also target promoters, thereby activating expression.Whether this mi RNA action also occurs in plants is unknown. In this study, we demonstrated that several mi RNAs regulate target promoters in Arabidopsis thaliana. For example, mi R5658 was predominantly present in the nucleus and activated the expression of AT3 G25290 directly by binding to its promoter. Our observations suggest that this mode of action may be a general feature of plant mi RNAs, and thus provide insight into the vital roles of plant mi RNAs in the nucleus.
论文参考文献
[1].A Proximal Promoter Region of Arabidopsis DREB2C Confers Tissue-specific Expression under Heat Stress[J]. Jihyun Je,Chieun Song,Jung Eun Hwang,Chae Oh Lim. Journal of Integrative Plant Biology.2012(09)[2].Abiotic Stresses and Phytohormones Regulate Expression of FAD2 Gene in Arabidopsis thaliana[J]. YUAN Si-wei1, 2*, WU Xue-long1*, LIU Zhi-hong1, LUO Hong-bing2 and HUANG Rui-zhi1 1 Key Laboratory of Plant Metabolic Engineering of Zhejiang Province/Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China 2 College of Agronomy, Hunan Agricultural University, Changsha 410128, P.R.China. Journal of Integrative Agriculture.2012(01)[3].TCP1 Modulates DWF4 Expression via Directly Interacting with the GGNCCC Motifs in the Promoter Region of DWF4 in Arabidopsis thaliana[J]. Yahu Gao,Dongzhi Zhang,Jia Li. Journal of Genetics and Genomics.2015(07)[4].Construction of a gene regulatory network for Arabidopsis based on metabolic pathway data[J]. JIAO QingJu1,YANG ZhongNan2 & HUANG JiFeng1 1 College of Information,Mechanical and Electrical Engineering,Shanghai Normal University,Shanghai 200234,China; 2 College of Life and Environmental Sciences,Shanghai Normal University,Shanghai 200234,China. Chinese Science Bulletin.2010(02)[5].A Preliminary Study on a Specifically Expressed Arabidopsis Promoter in Vascular Bundle[J]. 谢传晓,吴丽芳,余增亮,秦广雍,霍裕平,谷运红. Plasma Science & Technology.2003(02)[6].Characterization of Arabidopsis MYB transcription factor gene AtMYB17 and its possible regulation by LEAFY and AGL15[J]. Yunfei Zhang, Guangyu Cao, Li-Jia Qu, Hongya Gu National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China. 遗传学报.2009(02)[7].Cis-regulatory element-based genome-wide identification of DREB1/CBF targets in Arabidopsis[J]. Huan Zhao a, Weizhong Liu a,b, Yong Hu a, Xianglin Liu a, Yikun He a, a College of Life Science, Capital Normal University, Beijing 100037, China b College of Life Sciences, Shanxi Normal University, Linfen 041004, China. Progress in Natural Science.2008(05)[8].Overexpressing HRS1 Confers Hypersensitivity to Low Phosphate-Elicited Inhibition of Primary Root Growth in Arabidopsis thaliana[J]. Hong Liu1,2,Huixia Yang1,2,Chongming Wu1,2,Juanjuan Feng1,2,Xin Liu1,Huanju Qin1 and Daowen Wang1(1State Key Laboratory of Plant Cell and Chromosome Engineering,Institute of Genetics and Developmental Biology,the Chinese Academy of Sciences,Beijing 100101,China;2Graduate School of the Chinese Academy of Sciences,Beijing 100039,China). Journal of Integrative Plant Biology.2009(04)[9].Molecular cloning, expression and biochemical property analysis of AtKP1, a kinesin gene from Arabidopsis thaliana[J]. LI XuYan1*, WANG HaiQing1,2*, XU Tao1, CAO QinHong1, REN DongTao1 & LIU GuoQin1 1 State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100094, China; 2 Northwest Plateau Institute of Biology, the Chinese Academy of Sciences, Xining 810001, China. Chinese Science Bulletin.2007(10)[10].Phot2-regulated relocation of NPH3 mediates phototropic response to high-intensity blue light in Arabidopsis thaliana[J]. Xiang Zhao,Qingping Zhao,Chunye Xu,Jin Wang,Jindong Zhu,Baoshuan Shang,Xiao Zhang. Journal of Integrative Plant Biology.2018(07)
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：MicroRNAs transcriptionally regulate promoter activity in Arabidopsis thaliana论文
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