本文主要研究内容
作者乔向宇（2019）在《鲜驼乳和自然发酵驼乳对小鼠急性酒精性肝损伤的保护作用研究》一文中研究指出：酒精性肝病(Alcoholicliverdisease,ALD)是全球性的公共疾病卫生问题,发病率呈逐年上升的趋势。戒酒和营养支持是ALD的传统治疗手段且无副作用,近些年的研究建议通过膳食补充的手段限制早期ALD的发展。本研究选用一种简单易行的急性酒精性肝损伤小鼠模型,分别观察鲜驼乳和自然发酵驼乳的保护作用,并探讨相关作用机制,为把骆驼乳开发为一种天然的保肝护肝食品提供试验依据。采用短时间灌胃大量(7.3 g/kg)酒精的方法复制急性肝损伤小鼠模型,通过测定血清指标(ALT,AST,TNF-α,IL-1β 和 IL-6)、肝脏指标(MDA,SOD,GSH,GSH-Px,TG和TC)、观察肝脏组织病理学变化,来反映鲜驼乳和自然发酵驼乳对肝脏的保护作用,并基于组学的研究手段探讨了鲜驼乳发挥保肝护肝作用的可能机制。短时间内灌胃大量酒精会导致急性肝损伤,表现为模型组血清ALT和AST水平显著增加(p<0.05),肝脏MDA含量极显著升高(p<0.01),SOD和GSH-Px显著降低(p<0.05),肝脏脂质蓄积程度加重(p<0.01)。凋亡的肝细胞是正常组的4.06倍,肝脏病理切片显示出明显的水肿和大量炎性细胞浸润。自然发酵驼乳对小鼠急性酒精性肝损伤具有保护作用。与模型组比较,自然发酵驼乳组ALT活性显著下降(p<0.05);血清TNF-α和IL-lβ含量极显著降低(p<0.01);MDA含量显著下降(p<0.05),SOD及GSH-Px活性显著升高(p<0.05);TG含量极显著降低(p<0.01)。HE染色的结果显示小鼠肝组织中炎性细胞浸润明显减少,脂肪变性程度减轻。自然发酵驼乳的保肝护肝作用可能与降低小鼠体内炎症应答水平和改善肝脏氧化应激程度有关。鲜驼乳对小鼠急性酒精性肝损伤具有保护作用。与模型组相比,鲜驼乳组ALT和AST活性显著降低(p<0.05);肝脏氧化应激程度得到显著改善,脂质过氧化程度明显减轻(p<0.01),SOD和GSH-Px含量显著增加(p<0.05);血清促炎因子含量极显著降低(p<0.01);肝脏脂质蓄积减轻(p<0.05)。组织学结果显示,鲜驼乳组小鼠肝细胞水肿现象减轻,炎性细胞浸润减少,肝小叶结构逐渐恢复正常,脂肪变性程度减轻,肝细胞凋亡坏死明显减少。肝脏转录组学结果显示,由灌胃鲜驼乳而产生的差异基因,参与了氧化还原酶活性、血红素结合等生物学过程和IL-17、TNF等信号通路。鲜驼乳能够下调急性酒精性肝损伤小鼠肝脏中IL-17通路和TNF通路中与炎症反应相关的IL-1β和CXCL1等基因的表达,进而减轻肝组织的炎症应答。肠道内容物16S rDNA测序结果显示,鲜驼乳能够显著提高肠道中Lactobacillus的相对丰度,并降低Bacteroides、Alistipes以及Rikenellaceae_RC9_gut_group的含量,进而调节肠道菌群。鲜驼乳和自然发酵驼乳对小鼠急性酒精性肝损伤均具有保护作用,鲜驼乳发挥保肝护肝的作用与减轻肝脏炎症应答和改善肠道微生态平衡相关。
Abstract
jiu jing xing gan bing (Alcoholicliverdisease,ALD)shi quan qiu xing de gong gong ji bing wei sheng wen ti ,fa bing lv cheng zhu nian shang sheng de qu shi 。jie jiu he ying yang zhi chi shi ALDde chuan tong zhi liao shou duan ju mo fu zuo yong ,jin xie nian de yan jiu jian yi tong guo shan shi bu chong de shou duan xian zhi zao ji ALDde fa zhan 。ben yan jiu shua yong yi chong jian chan yi hang de ji xing jiu jing xing gan sun shang xiao shu mo xing ,fen bie guan cha xian tuo ru he zi ran fa jiao tuo ru de bao hu zuo yong ,bing tan tao xiang guan zuo yong ji zhi ,wei ba jia tuo ru kai fa wei yi chong tian ran de bao gan hu gan shi pin di gong shi yan yi ju 。cai yong duan shi jian guan wei da liang (7.3 g/kg)jiu jing de fang fa fu zhi ji xing gan sun shang xiao shu mo xing ,tong guo ce ding xie qing zhi biao (ALT,AST,TNF-α,IL-1β he IL-6)、gan zang zhi biao (MDA,SOD,GSH,GSH-Px,TGhe TC)、guan cha gan zang zu zhi bing li xue bian hua ,lai fan ying xian tuo ru he zi ran fa jiao tuo ru dui gan zang de bao hu zuo yong ,bing ji yu zu xue de yan jiu shou duan tan tao le xian tuo ru fa hui bao gan hu gan zuo yong de ke neng ji zhi 。duan shi jian nei guan wei da liang jiu jing hui dao zhi ji xing gan sun shang ,biao xian wei mo xing zu xie qing ALThe ASTshui ping xian zhe zeng jia (p<0.05),gan zang MDAhan liang ji xian zhe sheng gao (p<0.01),SODhe GSH-Pxxian zhe jiang di (p<0.05),gan zang zhi zhi xu ji cheng du jia chong (p<0.01)。diao wang de gan xi bao shi zheng chang zu de 4.06bei ,gan zang bing li qie pian xian shi chu ming xian de shui zhong he da liang yan xing xi bao jin run 。zi ran fa jiao tuo ru dui xiao shu ji xing jiu jing xing gan sun shang ju you bao hu zuo yong 。yu mo xing zu bi jiao ,zi ran fa jiao tuo ru zu ALThuo xing xian zhe xia jiang (p<0.05);xie qing TNF-αhe IL-lβhan liang ji xian zhe jiang di (p<0.01);MDAhan liang xian zhe xia jiang (p<0.05),SODji GSH-Pxhuo xing xian zhe sheng gao (p<0.05);TGhan liang ji xian zhe jiang di (p<0.01)。HEran se de jie guo xian shi xiao shu gan zu zhi zhong yan xing xi bao jin run ming xian jian shao ,zhi fang bian xing cheng du jian qing 。zi ran fa jiao tuo ru de bao gan hu gan zuo yong ke neng yu jiang di xiao shu ti nei yan zheng ying da shui ping he gai shan gan zang yang hua ying ji cheng du you guan 。xian tuo ru dui xiao shu ji xing jiu jing xing gan sun shang ju you bao hu zuo yong 。yu mo xing zu xiang bi ,xian tuo ru zu ALThe ASThuo xing xian zhe jiang di (p<0.05);gan zang yang hua ying ji cheng du de dao xian zhe gai shan ,zhi zhi guo yang hua cheng du ming xian jian qing (p<0.01),SODhe GSH-Pxhan liang xian zhe zeng jia (p<0.05);xie qing cu yan yin zi han liang ji xian zhe jiang di (p<0.01);gan zang zhi zhi xu ji jian qing (p<0.05)。zu zhi xue jie guo xian shi ,xian tuo ru zu xiao shu gan xi bao shui zhong xian xiang jian qing ,yan xing xi bao jin run jian shao ,gan xiao xie jie gou zhu jian hui fu zheng chang ,zhi fang bian xing cheng du jian qing ,gan xi bao diao wang huai si ming xian jian shao 。gan zang zhuai lu zu xue jie guo xian shi ,you guan wei xian tuo ru er chan sheng de cha yi ji yin ,can yu le yang hua hai yuan mei huo xing 、xie gong su jie ge deng sheng wu xue guo cheng he IL-17、TNFdeng xin hao tong lu 。xian tuo ru neng gou xia diao ji xing jiu jing xing gan sun shang xiao shu gan zang zhong IL-17tong lu he TNFtong lu zhong yu yan zheng fan ying xiang guan de IL-1βhe CXCL1deng ji yin de biao da ,jin er jian qing gan zu zhi de yan zheng ying da 。chang dao nei rong wu 16S rDNAce xu jie guo xian shi ,xian tuo ru neng gou xian zhe di gao chang dao zhong Lactobacillusde xiang dui feng du ,bing jiang di Bacteroides、Alistipesyi ji Rikenellaceae_RC9_gut_groupde han liang ,jin er diao jie chang dao jun qun 。xian tuo ru he zi ran fa jiao tuo ru dui xiao shu ji xing jiu jing xing gan sun shang jun ju you bao hu zuo yong ,xian tuo ru fa hui bao gan hu gan de zuo yong yu jian qing gan zang yan zheng ying da he gai shan chang dao wei sheng tai ping heng xiang guan 。
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论文作者分别是来自内蒙古农业大学的乔向宇，发表于刊物内蒙古农业大学2019-10-08论文，是一篇关于鲜驼乳论文,自然发酵驼乳论文,酒精性肝病论文,转录组学论文,肠道微生物论文,内蒙古农业大学2019-10-08论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自内蒙古农业大学2019-10-08论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：鲜驼乳论文; 自然发酵驼乳论文; 酒精性肝病论文; 转录组学论文; 肠道微生物论文; 内蒙古农业大学2019-10-08论文;