本文主要研究内容
作者涂小艳（2019）在《刺梨渣中种子油提取及其在囊泡制备上的应用研究》一文中研究指出：贵州刺梨酒生产中会产生大量的刺梨果渣,从果渣种子中提取的种子油富含亚油酸、亚麻酸等不饱和脂肪酸;不饱和脂肪酸囊泡在药物包埋和缓释方面都有重要应用。从将刺梨果渣变废为宝、节约资源的角度考虑,研究以贵州刺梨酒生产中的果渣为原料,进行种子油提取,利用尿素包合法纯化种子油中亚油酸及亚麻酸,将纯化后的脂肪酸用于混合脂肪酸囊泡的制备,最后应用于抗癌药物阿糖胞苷的缓释。研究可为刺梨果渣的回收利用提供新的思路。本文以刺梨果渣中种子为原料,采用微波法对刺梨种子油进行提取,通过响应面法,以出油率作为评价指标,对提取工艺进行优化;通过尿素包合法纯化种子油中的亚油酸及亚麻酸,利用气相色谱对种子油中的亚油酸、亚麻酸含量进行测定。根据回归分析,确定各因素对提取工艺的影响次序依次为:超声波>提取时间>液料比;求得的最佳提取参数为:液料比1:11,微波功率400w,提取时间40min。经验证实验平均出油率为12.51%,理论预测值与试验结果接近,说明优化工艺参数可信度高,优化工艺参数可信度高,具有参考价值。气相结果表明,未进行尿素包合前,刺梨种子油中亚油酸和亚麻酸总含量为26.52%(其中亚油酸含量为17.93%,亚麻酸含量为8.59%);进行尿素包合纯化后,亚油酸和亚麻酸的含量都提高了三倍以上,两者总含量为97.7%(亚油酸含量为65.38%,亚麻酸含量为32.32%),达到了纯化的目的,纯化前后混合脂肪酸(亚油酸及亚麻酸)比例保持不变,大约为2:1。将纯化后的混合脂肪酸作为脂肪酸囊泡的模板脂肪酸,采用表面张力法研究其在不同pH条件下的自组装行为,并采用酸碱平衡滴定实验分析混合脂肪酸自组装体形成囊泡的pH范围,采用动态光散色(DLS)分析其粒径分布,透射电子显微镜(TEM)观察其形貌。实验结果表明,当pH在13.0~9.0时,体系溶液外观清澈透明,混合脂肪酸主要以离子状态存在,离子间的静电排斥力使其自组装成胶束;当pH在9.0~8.0时,体系溶液外观表现为强烈乳光现象,混合脂肪酸分子与离子间的数量相当,两者以氢键作用力使其自组装成囊泡;当pH<8.0后,体系溶液外观浑浊甚至油水分离,溶液中主要是混合脂肪酸分子,使囊泡自组装体解体。用透射电镜和动态光结果表明pH 10.0时获得9 nm量级的球形胶束结构,pH 9.0时获得22 nm左右的囊泡结构,pH 8.0时获得40nm左右的类囊泡结构,说明混合脂肪酸能够发生pH响应自组装形成胶束或者囊泡等各种形貌和尺度的自组装体结构。混合脂肪酸囊泡对pH依赖性强,pH窗口很窄并且偏离生命体系适应的pH范围,限制其在药物包埋/缓释中的应用。为了迁移与扩张混合脂肪酸囊泡pH窗口,选择表面活性剂吐温80(Tween80)作为模板表面活性剂,加入到混合脂肪酸体系中,将pH窗口迁移至生命体系所适应的范围内。实验结果表明,吐温80加入到混合脂肪酸体系中,将囊泡的窗口由原来的pH 8.0~9.0迁移到pH 5.5~7.5,囊泡的pH窗口落在了生命体系所适应的pH范围内。由于吐温80中含有聚氧乙烯基(PEO)结构,能够在酸性条件下与混合脂肪酸中脂肪酸分子间产生氢键作用力,代替了原先在碱性范围内脂肪酸分子与离子间的氢键作用力,同时由于吐温80有增溶效果,增大混合脂肪酸在酸性条件下的溶解性,使混合脂肪酸囊泡在更宽的pH范围和更低的pH环境下形成并稳定。囊泡的粒径会随着pH的降低而增大,这是由于pH的变化将导致体系中形成囊泡的自组装单元发生变化,pH越低,混合脂肪酸体系中羧酸离子越少,羧酸离子间的静电排斥力减小,形成的囊泡结构越紧密,囊泡粒径就越大。将加入了吐温80的混合脂肪酸囊泡包覆抗癌药物阿糖胞苷,研究其包封率及其在人工肠液中的释放情况。研究结果表明,混合脂肪酸/吐温80复合囊泡对阿糖胞苷的包封率为42.07%;在人工肠液中,时间为9h,囊泡包封的阿糖胞苷释放率是40%,游离阿糖胞苷的释放率是80%,说明混合脂肪酸/吐温80复合囊泡对阿糖胞苷具有明显的缓释作用。将混合脂肪酸/吐温80复合囊泡与亚油酸/吐温80复合囊泡、亚麻酸/吐温80复合囊泡进行对比,发现三种复合囊泡的包封率及缓释效果相当。亚油酸和亚麻酸的分离过程比较复杂,费钱费力,如果不将二者分离,直接应用,可以简化工艺,提高经济效应。刺梨种子油中的亚油酸和亚麻酸纯化工艺简单,将纯化后的混合脂肪酸直接制备囊泡,应用于药物包埋/缓释,操作简单可行,具有很好的理论和实际应用价值。
Abstract
gui zhou ci li jiu sheng chan zhong hui chan sheng da liang de ci li guo zha ,cong guo zha chong zi zhong di qu de chong zi you fu han ya you suan 、ya ma suan deng bu bao he zhi fang suan ;bu bao he zhi fang suan nang pao zai yao wu bao mai he huan shi fang mian dou you chong yao ying yong 。cong jiang ci li guo zha bian fei wei bao 、jie yao zi yuan de jiao du kao lv ,yan jiu yi gui zhou ci li jiu sheng chan zhong de guo zha wei yuan liao ,jin hang chong zi you di qu ,li yong niao su bao ge fa chun hua chong zi you zhong ya you suan ji ya ma suan ,jiang chun hua hou de zhi fang suan yong yu hun ge zhi fang suan nang pao de zhi bei ,zui hou ying yong yu kang ai yao wu a tang bao gan de huan shi 。yan jiu ke wei ci li guo zha de hui shou li yong di gong xin de sai lu 。ben wen yi ci li guo zha zhong chong zi wei yuan liao ,cai yong wei bo fa dui ci li chong zi you jin hang di qu ,tong guo xiang ying mian fa ,yi chu you lv zuo wei ping jia zhi biao ,dui di qu gong yi jin hang you hua ;tong guo niao su bao ge fa chun hua chong zi you zhong de ya you suan ji ya ma suan ,li yong qi xiang se pu dui chong zi you zhong de ya you suan 、ya ma suan han liang jin hang ce ding 。gen ju hui gui fen xi ,que ding ge yin su dui di qu gong yi de ying xiang ci xu yi ci wei :chao sheng bo >di qu shi jian >ye liao bi ;qiu de de zui jia di qu can shu wei :ye liao bi 1:11,wei bo gong lv 400w,di qu shi jian 40min。jing yan zheng shi yan ping jun chu you lv wei 12.51%,li lun yu ce zhi yu shi yan jie guo jie jin ,shui ming you hua gong yi can shu ke xin du gao ,you hua gong yi can shu ke xin du gao ,ju you can kao jia zhi 。qi xiang jie guo biao ming ,wei jin hang niao su bao ge qian ,ci li chong zi you zhong ya you suan he ya ma suan zong han liang wei 26.52%(ji zhong ya you suan han liang wei 17.93%,ya ma suan han liang wei 8.59%);jin hang niao su bao ge chun hua hou ,ya you suan he ya ma suan de han liang dou di gao le san bei yi shang ,liang zhe zong han liang wei 97.7%(ya you suan han liang wei 65.38%,ya ma suan han liang wei 32.32%),da dao le chun hua de mu de ,chun hua qian hou hun ge zhi fang suan (ya you suan ji ya ma suan )bi li bao chi bu bian ,da yao wei 2:1。jiang chun hua hou de hun ge zhi fang suan zuo wei zhi fang suan nang pao de mo ban zhi fang suan ,cai yong biao mian zhang li fa yan jiu ji zai bu tong pHtiao jian xia de zi zu zhuang hang wei ,bing cai yong suan jian ping heng di ding shi yan fen xi hun ge zhi fang suan zi zu zhuang ti xing cheng nang pao de pHfan wei ,cai yong dong tai guang san se (DLS)fen xi ji li jing fen bu ,tou she dian zi xian wei jing (TEM)guan cha ji xing mao 。shi yan jie guo biao ming ,dang pHzai 13.0~9.0shi ,ti ji rong ye wai guan qing che tou ming ,hun ge zhi fang suan zhu yao yi li zi zhuang tai cun zai ,li zi jian de jing dian pai chi li shi ji zi zu zhuang cheng jiao shu ;dang pHzai 9.0~8.0shi ,ti ji rong ye wai guan biao xian wei jiang lie ru guang xian xiang ,hun ge zhi fang suan fen zi yu li zi jian de shu liang xiang dang ,liang zhe yi qing jian zuo yong li shi ji zi zu zhuang cheng nang pao ;dang pH<8.0hou ,ti ji rong ye wai guan hun zhuo shen zhi you shui fen li ,rong ye zhong zhu yao shi hun ge zhi fang suan fen zi ,shi nang pao zi zu zhuang ti jie ti 。yong tou she dian jing he dong tai guang jie guo biao ming pH 10.0shi huo de 9 nmliang ji de qiu xing jiao shu jie gou ,pH 9.0shi huo de 22 nmzuo you de nang pao jie gou ,pH 8.0shi huo de 40nmzuo you de lei nang pao jie gou ,shui ming hun ge zhi fang suan neng gou fa sheng pHxiang ying zi zu zhuang xing cheng jiao shu huo zhe nang pao deng ge chong xing mao he che du de zi zu zhuang ti jie gou 。hun ge zhi fang suan nang pao dui pHyi lai xing jiang ,pHchuang kou hen zhai bing ju pian li sheng ming ti ji kuo ying de pHfan wei ,xian zhi ji zai yao wu bao mai /huan shi zhong de ying yong 。wei le qian yi yu kuo zhang hun ge zhi fang suan nang pao pHchuang kou ,shua ze biao mian huo xing ji tu wen 80(Tween80)zuo wei mo ban biao mian huo xing ji ,jia ru dao hun ge zhi fang suan ti ji zhong ,jiang pHchuang kou qian yi zhi sheng ming ti ji suo kuo ying de fan wei nei 。shi yan jie guo biao ming ,tu wen 80jia ru dao hun ge zhi fang suan ti ji zhong ,jiang nang pao de chuang kou you yuan lai de pH 8.0~9.0qian yi dao pH 5.5~7.5,nang pao de pHchuang kou la zai le sheng ming ti ji suo kuo ying de pHfan wei nei 。you yu tu wen 80zhong han you ju yang yi xi ji (PEO)jie gou ,neng gou zai suan xing tiao jian xia yu hun ge zhi fang suan zhong zhi fang suan fen zi jian chan sheng qing jian zuo yong li ,dai ti le yuan xian zai jian xing fan wei nei zhi fang suan fen zi yu li zi jian de qing jian zuo yong li ,tong shi you yu tu wen 80you zeng rong xiao guo ,zeng da hun ge zhi fang suan zai suan xing tiao jian xia de rong jie xing ,shi hun ge zhi fang suan nang pao zai geng kuan de pHfan wei he geng di de pHhuan jing xia xing cheng bing wen ding 。nang pao de li jing hui sui zhao pHde jiang di er zeng da ,zhe shi you yu pHde bian hua jiang dao zhi ti ji zhong xing cheng nang pao de zi zu zhuang chan yuan fa sheng bian hua ,pHyue di ,hun ge zhi fang suan ti ji zhong suo suan li zi yue shao ,suo suan li zi jian de jing dian pai chi li jian xiao ,xing cheng de nang pao jie gou yue jin mi ,nang pao li jing jiu yue da 。jiang jia ru le tu wen 80de hun ge zhi fang suan nang pao bao fu kang ai yao wu a tang bao gan ,yan jiu ji bao feng lv ji ji zai ren gong chang ye zhong de shi fang qing kuang 。yan jiu jie guo biao ming ,hun ge zhi fang suan /tu wen 80fu ge nang pao dui a tang bao gan de bao feng lv wei 42.07%;zai ren gong chang ye zhong ,shi jian wei 9h,nang pao bao feng de a tang bao gan shi fang lv shi 40%,you li a tang bao gan de shi fang lv shi 80%,shui ming hun ge zhi fang suan /tu wen 80fu ge nang pao dui a tang bao gan ju you ming xian de huan shi zuo yong 。jiang hun ge zhi fang suan /tu wen 80fu ge nang pao yu ya you suan /tu wen 80fu ge nang pao 、ya ma suan /tu wen 80fu ge nang pao jin hang dui bi ,fa xian san chong fu ge nang pao de bao feng lv ji huan shi xiao guo xiang dang 。ya you suan he ya ma suan de fen li guo cheng bi jiao fu za ,fei qian fei li ,ru guo bu jiang er zhe fen li ,zhi jie ying yong ,ke yi jian hua gong yi ,di gao jing ji xiao ying 。ci li chong zi you zhong de ya you suan he ya ma suan chun hua gong yi jian chan ,jiang chun hua hou de hun ge zhi fang suan zhi jie zhi bei nang pao ,ying yong yu yao wu bao mai /huan shi ,cao zuo jian chan ke hang ,ju you hen hao de li lun he shi ji ying yong jia zhi 。
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论文详细介绍
论文作者分别是来自贵州大学的涂小艳，发表于刊物贵州大学2019-07-16论文，是一篇关于刺梨渣论文,刺梨种子油论文,脂肪酸囊泡论文,吐温论文,阿糖胞苷论文,贵州大学2019-07-16论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自贵州大学2019-07-16论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。