本文主要研究内容
作者王琛（2019）在《黄瓜根发育异常突变体及其候选基因的鉴定》一文中研究指出：黄瓜（Cucumis sativus L.2n=2x=14）为葫芦科甜瓜属植物,是世界上主要的蔬菜作物,也是我国广泛栽培的设施蔬菜作物之一。根作为植物的营养器官,在植株整个生长发育历程中发挥着至关重要的作用。根是黄瓜植株的重要组成部分,具有吸收营养物质和水分等功能。黄瓜根发育异常表型是研究植株根发育机理及培育优良品种的理想性状,但是相关研究报道较少。本研究在前期构建黄瓜EMS诱变突变体库基础上构建了黄瓜EMS诱变根发育异常突变体‘Csrod（Cucumis sativus root dysplasia）’的M3代遗传群体,对其进行表型鉴定,并利用MutMap+技术对突变表型和野生表型混池分别进行重测序分析,对根发育异常候选基因进行了初步定位,运用KASP基因分型技术对候选SNP在M3代群体中进行了鉴定,并最终确定了黄瓜EMS诱变根发育异常候选基因。主要结果如下:（1）表型鉴定结果表明,黄瓜根发育异常突变体‘Csrod’在根发育等方面与野生型存在显著差异。根发育异常突变体‘Csrod’根部较野生型生长慢、只能生长至1-2 cm,植株生长至14 d左右,根与子叶分离,为致死表型。（2）为了鉴定黄瓜根发育异常突变体‘Csrod’候选基因,将M2代野生型表型单株进行自交得到M3代群体,其中一个有性状分离的M3群体中有11株为突变体表型单株,93株表现为野生型表型。利用MutMap+方法进行极端性状混池重测序分析,选取M3代中突变表型单株11株和野生型表型单株34株,提取等量DNA进行混合,分别构建突变体表型池和野生型表型池进行混池重测序,并与黄瓜基因组序列进行比对,依据SNP-index以及突变位点对基因的影响进行综合分析,筛选到位于黄瓜第3号染色体的5个候选SNP,其中4个候选SNP位于基因外显子区,另外1个候选SNP则位于终止密码子区。结合KASP基因分型技术,在M3代群体中发现基因ID为Csa3G104930包含的SNP的基因型与M3群体单株表型共分离,表明Csa3G104930为调控黄瓜根发育异常的候选基因。序列分析结果表明Csa3G104930基因全长3876 bp,编码831个氨基酸,参与3-脱氧-D-甘露糖-八磺酸盐合成酶及酰基神经氨酸蛋白家族的胞苷酰基转移酶的合成,因此将黄瓜根发育异常突变体候选基因命名为Cscks（Cucumis sativus CMP-KDO synthetase）。本研究初步鉴定出一个参与调控黄瓜根部发育的新基因,并对其基因功能进行了初步探讨,下一步将对该基因功能及分子机制进行深入研究,进而对黄瓜营养生长、新品种培育等研究提供参考。
Abstract
huang gua （Cucumis sativus L.2n=2x=14）wei hu lu ke tian gua shu zhi wu ,shi shi jie shang zhu yao de shu cai zuo wu ,ye shi wo guo an fan zai pei de she shi shu cai zuo wu zhi yi 。gen zuo wei zhi wu de ying yang qi guan ,zai zhi zhu zheng ge sheng chang fa yo li cheng zhong fa hui zhao zhi guan chong yao de zuo yong 。gen shi huang gua zhi zhu de chong yao zu cheng bu fen ,ju you xi shou ying yang wu zhi he shui fen deng gong neng 。huang gua gen fa yo yi chang biao xing shi yan jiu zhi zhu gen fa yo ji li ji pei yo you liang pin chong de li xiang xing zhuang ,dan shi xiang guan yan jiu bao dao jiao shao 。ben yan jiu zai qian ji gou jian huang gua EMSyou bian tu bian ti ku ji chu shang gou jian le huang gua EMSyou bian gen fa yo yi chang tu bian ti ‘Csrod（Cucumis sativus root dysplasia）’de M3dai wei chuan qun ti ,dui ji jin hang biao xing jian ding ,bing li yong MutMap+ji shu dui tu bian biao xing he ye sheng biao xing hun chi fen bie jin hang chong ce xu fen xi ,dui gen fa yo yi chang hou shua ji yin jin hang le chu bu ding wei ,yun yong KASPji yin fen xing ji shu dui hou shua SNPzai M3dai qun ti zhong jin hang le jian ding ,bing zui zhong que ding le huang gua EMSyou bian gen fa yo yi chang hou shua ji yin 。zhu yao jie guo ru xia :（1）biao xing jian ding jie guo biao ming ,huang gua gen fa yo yi chang tu bian ti ‘Csrod’zai gen fa yo deng fang mian yu ye sheng xing cun zai xian zhe cha yi 。gen fa yo yi chang tu bian ti ‘Csrod’gen bu jiao ye sheng xing sheng chang man 、zhi neng sheng chang zhi 1-2 cm,zhi zhu sheng chang zhi 14 dzuo you ,gen yu zi xie fen li ,wei zhi si biao xing 。（2）wei le jian ding huang gua gen fa yo yi chang tu bian ti ‘Csrod’hou shua ji yin ,jiang M2dai ye sheng xing biao xing chan zhu jin hang zi jiao de dao M3dai qun ti ,ji zhong yi ge you xing zhuang fen li de M3qun ti zhong you 11zhu wei tu bian ti biao xing chan zhu ,93zhu biao xian wei ye sheng xing biao xing 。li yong MutMap+fang fa jin hang ji duan xing zhuang hun chi chong ce xu fen xi ,shua qu M3dai zhong tu bian biao xing chan zhu 11zhu he ye sheng xing biao xing chan zhu 34zhu ,di qu deng liang DNAjin hang hun ge ,fen bie gou jian tu bian ti biao xing chi he ye sheng xing biao xing chi jin hang hun chi chong ce xu ,bing yu huang gua ji yin zu xu lie jin hang bi dui ,yi ju SNP-indexyi ji tu bian wei dian dui ji yin de ying xiang jin hang zeng ge fen xi ,shai shua dao wei yu huang gua di 3hao ran se ti de 5ge hou shua SNP,ji zhong 4ge hou shua SNPwei yu ji yin wai xian zi ou ,ling wai 1ge hou shua SNPze wei yu zhong zhi mi ma zi ou 。jie ge KASPji yin fen xing ji shu ,zai M3dai qun ti zhong fa xian ji yin IDwei Csa3G104930bao han de SNPde ji yin xing yu M3qun ti chan zhu biao xing gong fen li ,biao ming Csa3G104930wei diao kong huang gua gen fa yo yi chang de hou shua ji yin 。xu lie fen xi jie guo biao ming Csa3G104930ji yin quan chang 3876 bp,bian ma 831ge an ji suan ,can yu 3-tuo yang -D-gan lou tang -ba huang suan yan ge cheng mei ji xian ji shen jing an suan dan bai jia zu de bao gan xian ji zhuai yi mei de ge cheng ,yin ci jiang huang gua gen fa yo yi chang tu bian ti hou shua ji yin ming ming wei Cscks（Cucumis sativus CMP-KDO synthetase）。ben yan jiu chu bu jian ding chu yi ge can yu diao kong huang gua gen bu fa yo de xin ji yin ,bing dui ji ji yin gong neng jin hang le chu bu tan tao ,xia yi bu jiang dui gai ji yin gong neng ji fen zi ji zhi jin hang shen ru yan jiu ,jin er dui huang gua ying yang sheng chang 、xin pin chong pei yo deng yan jiu di gong can kao 。
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论文详细介绍
论文作者分别是来自东北农业大学的王琛，发表于刊物东北农业大学2019-08-27论文，是一篇关于黄瓜论文,根发育突变体论文,诱变论文,基因分型论文,东北农业大学2019-08-27论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自东北农业大学2019-08-27论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：黄瓜论文; 根发育突变体论文; 诱变论文; 基因分型论文; 东北农业大学2019-08-27论文;