本文主要研究内容
作者陈延儒（2019）在《采后硝普钠处理对苹果果实品质、莽草酸和苯丙烷代谢途径的影响》一文中研究指出：衰老和腐烂是加速苹果果实采后品质劣变和烂损的重要原因。硝普钠(SNP)是外源一氧化氮(NO)的供体,可通过还原反应自发分解并释放NO,是研究NO特性及功能的重要载体。本文以“富士”苹果果实为材料,采后经1.0 mmol/L的SNP溶液浸泡10 min,研究常温贮藏期间苹果果实品质指标、蔗糖代谢、莽草酸及苯丙烷代谢途径的变化。得到以下主要结果:1.采后SNP处理显著延缓了贮藏期间苹果果实呼吸高峰出现的时间,并降低了果实的呼吸强度;保持了较高的果实硬度、可滴定酸(TA)及抗坏血酸(AsA)的含量;减少了可溶性固形物含量(SSC)的消耗;并延缓了丙二醛(MDA)和可溶性糖含量的上升。但对果实的失重率没有显著影响。2.采后SNP处理显著抑制了苹果果实蔗糖代谢中转化酶和蔗糖合酶-分解方向(SS-c)活性,提高了蔗糖磷酸合成酶(SPS)和蔗糖合酶-合成方向(SS-s)活性以及蔗糖代谢酶净活性;SNP处理还上调了果实MdNI、蔗糖合酶(MdSS)和MdSPS基因的表达,同时还上调了己糖代谢中已糖激酶(MdHK)和果糖激酶(MdFK)基因的表达。3.采后SNP处理显著降低了损伤接种扩展青霉(Penicillium expansum)苹果果实的病斑直径,并上调了莽草酸途径中3-脱氢奎尼酸合成酶(MdDHQS)、莽草酸脱氢酶(MdSKDH)、莽草酸激酶(MdSK)和5-烯醇式丙酮酰莽草酸-3-磷酸合酶(MdEPSPS)基因的表达;促进了贮藏前期莽草酸和色氨酸的积累,且提高了整个贮藏期间酪氨酸和苯丙氨酸的含量。4.采后SNP处理显著上调了苯丙烷代谢途径中苯丙氨酸解氨酶(MdPAL)、肉桂酰乙醇脱氢酶(MdC4H)、4-香豆酸辅酶A连接酶(Md4CL)及查尔酮异构酶(MdCHI)基因的表达,并提高了贮藏期间类黄酮、总酚和木质素的含量。这些结果表明,采后SNP处理可以通过调节苹果果实中蔗糖代谢相关酶活性和基因表达来延缓常温贮藏过程中果实品质的下降,并可以通过调控莽草酸和苯丙烷代谢途径以提高果实对青霉病的抗性。
Abstract
cui lao he fu lan shi jia su ping guo guo shi cai hou pin zhi lie bian he lan sun de chong yao yuan yin 。xiao pu na (SNP)shi wai yuan yi yang hua dan (NO)de gong ti ,ke tong guo hai yuan fan ying zi fa fen jie bing shi fang NO,shi yan jiu NOte xing ji gong neng de chong yao zai ti 。ben wen yi “fu shi ”ping guo guo shi wei cai liao ,cai hou jing 1.0 mmol/Lde SNProng ye jin pao 10 min,yan jiu chang wen zhu cang ji jian ping guo guo shi pin zhi zhi biao 、zhe tang dai xie 、mang cao suan ji ben bing wan dai xie tu jing de bian hua 。de dao yi xia zhu yao jie guo :1.cai hou SNPchu li xian zhe yan huan le zhu cang ji jian ping guo guo shi hu xi gao feng chu xian de shi jian ,bing jiang di le guo shi de hu xi jiang du ;bao chi le jiao gao de guo shi ying du 、ke di ding suan (TA)ji kang huai xie suan (AsA)de han liang ;jian shao le ke rong xing gu xing wu han liang (SSC)de xiao hao ;bing yan huan le bing er quan (MDA)he ke rong xing tang han liang de shang sheng 。dan dui guo shi de shi chong lv mei you xian zhe ying xiang 。2.cai hou SNPchu li xian zhe yi zhi le ping guo guo shi zhe tang dai xie zhong zhuai hua mei he zhe tang ge mei -fen jie fang xiang (SS-c)huo xing ,di gao le zhe tang lin suan ge cheng mei (SPS)he zhe tang ge mei -ge cheng fang xiang (SS-s)huo xing yi ji zhe tang dai xie mei jing huo xing ;SNPchu li hai shang diao le guo shi MdNI、zhe tang ge mei (MdSS)he MdSPSji yin de biao da ,tong shi hai shang diao le ji tang dai xie zhong yi tang ji mei (MdHK)he guo tang ji mei (MdFK)ji yin de biao da 。3.cai hou SNPchu li xian zhe jiang di le sun shang jie chong kuo zhan qing mei (Penicillium expansum)ping guo guo shi de bing ban zhi jing ,bing shang diao le mang cao suan tu jing zhong 3-tuo qing kui ni suan ge cheng mei (MdDHQS)、mang cao suan tuo qing mei (MdSKDH)、mang cao suan ji mei (MdSK)he 5-xi chun shi bing tong xian mang cao suan -3-lin suan ge mei (MdEPSPS)ji yin de biao da ;cu jin le zhu cang qian ji mang cao suan he se an suan de ji lei ,ju di gao le zheng ge zhu cang ji jian lao an suan he ben bing an suan de han liang 。4.cai hou SNPchu li xian zhe shang diao le ben bing wan dai xie tu jing zhong ben bing an suan jie an mei (MdPAL)、rou gui xian yi chun tuo qing mei (MdC4H)、4-xiang dou suan fu mei Alian jie mei (Md4CL)ji cha er tong yi gou mei (MdCHI)ji yin de biao da ,bing di gao le zhu cang ji jian lei huang tong 、zong fen he mu zhi su de han liang 。zhe xie jie guo biao ming ,cai hou SNPchu li ke yi tong guo diao jie ping guo guo shi zhong zhe tang dai xie xiang guan mei huo xing he ji yin biao da lai yan huan chang wen zhu cang guo cheng zhong guo shi pin zhi de xia jiang ,bing ke yi tong guo diao kong mang cao suan he ben bing wan dai xie tu jing yi di gao guo shi dui qing mei bing de kang xing 。
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论文详细介绍
论文作者分别是来自渤海大学的陈延儒，发表于刊物渤海大学2019-10-16论文，是一篇关于硝普钠论文,苹果论文,品质论文,抗病性论文,莽草酸途径论文,渤海大学2019-10-16论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自渤海大学2019-10-16论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。