本文主要研究内容
作者梁燕（2019）在《马铃薯疮痂病生防菌筛选及其生防机制的初步研究》一文中研究指出：马铃薯疮痂病(potato common scab)是世界各马铃薯产区普遍发生的一种重要土传病害,该病主要由链霉菌(Streptomycesspp.)引起。该病严重影响马铃薯的外观品质,降低薯块的商品价值,且给马铃薯的生产造成很大威胁。对微型薯生产的影响尤为严重,给马铃薯的生产造成很大威胁。本研究从生物防治角度出发,筛选对马铃薯疮痂病原菌具有生防作用的微生物资源,为有效防治马铃薯疮痂病奠定基础,为生防菌剂的研发提供理论依据。研究结果如下:1.本试验是从4个马铃薯疮痂病发生严重的地块采土样和病薯进行生防菌的分离,共得到53株细菌,经过筛选得到6株对疮痂病原菌PS1具有不同拮抗作用的拮抗菌,通过拮抗菌活性测定、盆栽和田间防效测定,最终筛选出2株防治效果最佳且相对稳定,又能增产的菌N3和N23。菌株N3的田间防效达75.15%,增产效果最可达10.03%;菌株N23的田间防效达73.26%,增产效果可达8.01%。比较了几个菌株浸种和沟施两种接种方式,施用生防菌株N3的最佳接种方式为沟施,防效为75.15%;施用生防菌株N23的最佳接种方式为浸种,防效为73.26%。经抑菌谱测定,菌株N3对马铃薯黑痣病菌立枯丝核菌(Rhizoctonia solani AG-3)的生长有非常好的抑制作用,抑制率为79.0%,对马铃薯枯萎病菌尖孢镰刀菌(Fusariu oxysopoyum)、马铃薯黄萎病菌大丽轮枝菌(Verticillium dahliae)、马铃薯黑胫病菌欧文氏菌属马铃薯黑胫亚种(Pectobacteaatm atrosepticum)几种病原菌没有抑制作用。2.根据形态学、生理生化和分子生物学鉴定技术,确定生防菌株N3为枯草芽孢杆菌(Bacillussubtis),N23为甲基营养型芽孢杆菌(Bacillus methylotrophicus)。3.生防菌株N3和N23的生物学特性研究结果表明,N3生长的最适碳源为可溶性淀粉,最适氮源为硫酸铵,最适生长pH为7.5,最适生长温度为28℃;N23生长的最适碳源为可溶性淀粉,最适氮源为硝酸氨,最适生长pH为7.5,最适生长温度为 32℃。4.生防菌株N3和N23的生防机制研究结果表明,未发现2株菌产生挥发性物质对病原菌PS1的生长有抑制作用,非挥发性物质对PS1生长具有明显的抑制作用,菌株N3对疮痂病原菌的抑制率为69.23%,菌株N23对疮痂病原菌的抑制率达到61.23%。室内马铃薯种薯萌发和生长试验及两年田间试验结果显示,两株菌均能增加马铃薯出苗率,提高生长势,具有显著促生作用,并能增加产量。
Abstract
ma ling shu chuang jia bing (potato common scab)shi shi jie ge ma ling shu chan ou pu bian fa sheng de yi chong chong yao tu chuan bing hai ,gai bing zhu yao you lian mei jun (Streptomycesspp.)yin qi 。gai bing yan chong ying xiang ma ling shu de wai guan pin zhi ,jiang di shu kuai de shang pin jia zhi ,ju gei ma ling shu de sheng chan zao cheng hen da wei xie 。dui wei xing shu sheng chan de ying xiang you wei yan chong ,gei ma ling shu de sheng chan zao cheng hen da wei xie 。ben yan jiu cong sheng wu fang zhi jiao du chu fa ,shai shua dui ma ling shu chuang jia bing yuan jun ju you sheng fang zuo yong de wei sheng wu zi yuan ,wei you xiao fang zhi ma ling shu chuang jia bing dian ding ji chu ,wei sheng fang jun ji de yan fa di gong li lun yi ju 。yan jiu jie guo ru xia :1.ben shi yan shi cong 4ge ma ling shu chuang jia bing fa sheng yan chong de de kuai cai tu yang he bing shu jin hang sheng fang jun de fen li ,gong de dao 53zhu xi jun ,jing guo shai shua de dao 6zhu dui chuang jia bing yuan jun PS1ju you bu tong jie kang zuo yong de jie kang jun ,tong guo jie kang jun huo xing ce ding 、pen zai he tian jian fang xiao ce ding ,zui zhong shai shua chu 2zhu fang zhi xiao guo zui jia ju xiang dui wen ding ,you neng zeng chan de jun N3he N23。jun zhu N3de tian jian fang xiao da 75.15%,zeng chan xiao guo zui ke da 10.03%;jun zhu N23de tian jian fang xiao da 73.26%,zeng chan xiao guo ke da 8.01%。bi jiao le ji ge jun zhu jin chong he gou shi liang chong jie chong fang shi ,shi yong sheng fang jun zhu N3de zui jia jie chong fang shi wei gou shi ,fang xiao wei 75.15%;shi yong sheng fang jun zhu N23de zui jia jie chong fang shi wei jin chong ,fang xiao wei 73.26%。jing yi jun pu ce ding ,jun zhu N3dui ma ling shu hei zhi bing jun li ku si he jun (Rhizoctonia solani AG-3)de sheng chang you fei chang hao de yi zhi zuo yong ,yi zhi lv wei 79.0%,dui ma ling shu ku wei bing jun jian bao lian dao jun (Fusariu oxysopoyum)、ma ling shu huang wei bing jun da li lun zhi jun (Verticillium dahliae)、ma ling shu hei jing bing jun ou wen shi jun shu ma ling shu hei jing ya chong (Pectobacteaatm atrosepticum)ji chong bing yuan jun mei you yi zhi zuo yong 。2.gen ju xing tai xue 、sheng li sheng hua he fen zi sheng wu xue jian ding ji shu ,que ding sheng fang jun zhu N3wei ku cao ya bao gan jun (Bacillussubtis),N23wei jia ji ying yang xing ya bao gan jun (Bacillus methylotrophicus)。3.sheng fang jun zhu N3he N23de sheng wu xue te xing yan jiu jie guo biao ming ,N3sheng chang de zui kuo tan yuan wei ke rong xing dian fen ,zui kuo dan yuan wei liu suan an ,zui kuo sheng chang pHwei 7.5,zui kuo sheng chang wen du wei 28℃;N23sheng chang de zui kuo tan yuan wei ke rong xing dian fen ,zui kuo dan yuan wei xiao suan an ,zui kuo sheng chang pHwei 7.5,zui kuo sheng chang wen du wei 32℃。4.sheng fang jun zhu N3he N23de sheng fang ji zhi yan jiu jie guo biao ming ,wei fa xian 2zhu jun chan sheng hui fa xing wu zhi dui bing yuan jun PS1de sheng chang you yi zhi zuo yong ,fei hui fa xing wu zhi dui PS1sheng chang ju you ming xian de yi zhi zuo yong ,jun zhu N3dui chuang jia bing yuan jun de yi zhi lv wei 69.23%,jun zhu N23dui chuang jia bing yuan jun de yi zhi lv da dao 61.23%。shi nei ma ling shu chong shu meng fa he sheng chang shi yan ji liang nian tian jian shi yan jie guo xian shi ,liang zhu jun jun neng zeng jia ma ling shu chu miao lv ,di gao sheng chang shi ,ju you xian zhe cu sheng zuo yong ,bing neng zeng jia chan liang 。
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论文详细介绍
论文作者分别是来自内蒙古农业大学的梁燕，发表于刊物内蒙古农业大学2019-10-08论文，是一篇关于马铃薯疮痂病论文,生防菌论文,枯草芽孢杆菌论文,甲基营养型芽孢杆菌论文,生防机制论文,内蒙古农业大学2019-10-08论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自内蒙古农业大学2019-10-08论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：马铃薯疮痂病论文; 生防菌论文; 枯草芽孢杆菌论文; 甲基营养型芽孢杆菌论文; 生防机制论文; 内蒙古农业大学2019-10-08论文;