本文主要研究内容
作者张军耀（2019）在《富硒黑茶多糖的提取分离纯化及性质研究》一文中研究指出：硒多糖是一种兼具硒与多糖双重活性的有机硒化合物,因其生物利用度高、毒性低而成为新的研究热点,具有抗氧化、抗肿瘤、提高机体免疫等多种活性。本论文的研究对象-富硒茶多糖,是从富硒黑茶中提取分离得到的硒多糖,不仅具有多糖的生物活性,且具有良好的应用前景,但当前国内外对其研究报道比较少,尤其是关于富硒黑茶多糖的结构特征及活性上的研究。本论文以湖北富硒黑茶为原料,采用热水浸提-乙醇沉淀的方法提取富硒黑茶多糖,并利用DEAE-Sepharose FF分离纯化硒多糖,而后对硒多糖进行了理化组成分析,结构表征及体外活性的研究。主要研究结果如下:(1)通过对富硒黑茶原料的基本成分分析,富硒黑茶的硒含量符合国家对于富硒食品定义中硒含量的规定要求。富硒黑茶多糖提取单因素实验结果表明:提取温度为90℃最合适,提取时间为2h最佳,提取料液比为1:20最优。而后通过响应面设计软件对富硒黑茶多糖提取工艺进行优化设计,得到最佳提取温度为94.2℃,最佳提取时间为1.64 h,最佳提取料液比为1:20.56,理论得率为6.0%。通过响应面分析可知提取时间和提取料液比对多糖得率的影响为极显著(P<0.01),提取温度对多糖得率的影响为显著P=0.0393(P<0.05)。由于实际操作的限制,因而在验证性实验中最终设定提取温度为95℃,提取时间为2h,提取料液比为1:20。在这样的提取条件下多糖得率为6.00%左右,与理论得率误差在2%范围内波动,符合设计要求。提取后的富硒黑茶粗多糖经DEAE Sepharose Fast Flow分离,最后经UV在490nm下测定吸光度值。通过收集不同洗脱时间节点下的组分,得到6组洗脱组分峰。洗脱分离的各个组分酸性糖测定实验表明,洗脱的六个组分的糖醛酸含量均较高,是典型的酸性多糖。(2)通过对富硒黑茶多糖粗品和6种纯化组分进行紫外全波长扫描得出富硒黑茶多糖粗品和分离洗脱组分均在280nm处无明显吸收峰,说明富硒黑茶多糖均不含有芳香族氨基酸。而通过聚丙烯氨酰胺凝胶电泳可知富硒黑茶多糖粗品和6种纯化组分均不含游离的蛋白。红外光谱结果表明富硒黑茶多糖以及6种纯化组分都具有典型的多糖类吸收峰。凝胶反渗透色谱结果表明富硒黑茶多糖具有较高的分子量且含有酰胺键,可初步推断其为糖蛋白结合物。离子色谱结果表明富硒黑茶多糖纯化组分中单糖组成单糖含量最高为11种,最低为10种。由扫描电镜和原子力显微镜的结果可知,富硒黑茶多糖的纯化组分在表观结构、表面平整度方面均具有明显差异。XRD衍射分析可知富硒黑茶多糖粗品和纯化组分在晶体形成方面差异显著。而DSC分析结果表明富硒黑茶多糖纯化组分之间热特性变化均具有显著差异。(3)ABTS~+自由基清除实验表明,富硒黑茶多糖粗品及各纯化组分对ABTS~+自由基清除活性与对照组相比差异显著,但各组分之间无明显差异。超氧阴离子自由基清除实验表明,富硒黑茶多糖粗品及各纯化组分与对照组相比差异显著,但各组分之间无显著差异。α-葡萄糖苷酶抑制率实验结果表明富硒黑茶多糖粗品及各纯化组分均对α-葡萄糖苷酶有显著抑制活性并且和阳性对照阿卡波糖组相比差异不显著。通过人工胃液体外消化实验可知经提取纯化获得的富硒黑茶多糖不易被胃液消化。而经人工肠液的体外消化实验可知富硒黑茶多糖粗品及其组分亦不易被肠液消化。(4)通过对高得率、高平整度组分4进一步分离纯化,得到了均一组分,并对均一组分进行超氧阴离子自由基和ABTS~+自由基的清除率分析。结果表明自由基清除活性与均一组分浓度呈正相关,且浓度在1mg/ml时ABTS自由基清除率达到80%,超氧阴离子自由基能达到70%。
Abstract
xi duo tang shi yi chong jian ju xi yu duo tang shuang chong huo xing de you ji xi hua ge wu ,yin ji sheng wu li yong du gao 、du xing di er cheng wei xin de yan jiu re dian ,ju you kang yang hua 、kang zhong liu 、di gao ji ti mian yi deng duo chong huo xing 。ben lun wen de yan jiu dui xiang -fu xi cha duo tang ,shi cong fu xi hei cha zhong di qu fen li de dao de xi duo tang ,bu jin ju you duo tang de sheng wu huo xing ,ju ju you liang hao de ying yong qian jing ,dan dang qian guo nei wai dui ji yan jiu bao dao bi jiao shao ,you ji shi guan yu fu xi hei cha duo tang de jie gou te zheng ji huo xing shang de yan jiu 。ben lun wen yi hu bei fu xi hei cha wei yuan liao ,cai yong re shui jin di -yi chun chen dian de fang fa di qu fu xi hei cha duo tang ,bing li yong DEAE-Sepharose FFfen li chun hua xi duo tang ,er hou dui xi duo tang jin hang le li hua zu cheng fen xi ,jie gou biao zheng ji ti wai huo xing de yan jiu 。zhu yao yan jiu jie guo ru xia :(1)tong guo dui fu xi hei cha yuan liao de ji ben cheng fen fen xi ,fu xi hei cha de xi han liang fu ge guo jia dui yu fu xi shi pin ding yi zhong xi han liang de gui ding yao qiu 。fu xi hei cha duo tang di qu chan yin su shi yan jie guo biao ming :di qu wen du wei 90℃zui ge kuo ,di qu shi jian wei 2hzui jia ,di qu liao ye bi wei 1:20zui you 。er hou tong guo xiang ying mian she ji ruan jian dui fu xi hei cha duo tang di qu gong yi jin hang you hua she ji ,de dao zui jia di qu wen du wei 94.2℃,zui jia di qu shi jian wei 1.64 h,zui jia di qu liao ye bi wei 1:20.56,li lun de lv wei 6.0%。tong guo xiang ying mian fen xi ke zhi di qu shi jian he di qu liao ye bi dui duo tang de lv de ying xiang wei ji xian zhe (P<0.01),di qu wen du dui duo tang de lv de ying xiang wei xian zhe P=0.0393(P<0.05)。you yu shi ji cao zuo de xian zhi ,yin er zai yan zheng xing shi yan zhong zui zhong she ding di qu wen du wei 95℃,di qu shi jian wei 2h,di qu liao ye bi wei 1:20。zai zhe yang de di qu tiao jian xia duo tang de lv wei 6.00%zuo you ,yu li lun de lv wu cha zai 2%fan wei nei bo dong ,fu ge she ji yao qiu 。di qu hou de fu xi hei cha cu duo tang jing DEAE Sepharose Fast Flowfen li ,zui hou jing UVzai 490nmxia ce ding xi guang du zhi 。tong guo shou ji bu tong xi tuo shi jian jie dian xia de zu fen ,de dao 6zu xi tuo zu fen feng 。xi tuo fen li de ge ge zu fen suan xing tang ce ding shi yan biao ming ,xi tuo de liu ge zu fen de tang quan suan han liang jun jiao gao ,shi dian xing de suan xing duo tang 。(2)tong guo dui fu xi hei cha duo tang cu pin he 6chong chun hua zu fen jin hang zi wai quan bo chang sao miao de chu fu xi hei cha duo tang cu pin he fen li xi tuo zu fen jun zai 280nmchu mo ming xian xi shou feng ,shui ming fu xi hei cha duo tang jun bu han you fang xiang zu an ji suan 。er tong guo ju bing xi an xian an ning jiao dian yong ke zhi fu xi hei cha duo tang cu pin he 6chong chun hua zu fen jun bu han you li de dan bai 。gong wai guang pu jie guo biao ming fu xi hei cha duo tang yi ji 6chong chun hua zu fen dou ju you dian xing de duo tang lei xi shou feng 。ning jiao fan shen tou se pu jie guo biao ming fu xi hei cha duo tang ju you jiao gao de fen zi liang ju han you xian an jian ,ke chu bu tui duan ji wei tang dan bai jie ge wu 。li zi se pu jie guo biao ming fu xi hei cha duo tang chun hua zu fen zhong chan tang zu cheng chan tang han liang zui gao wei 11chong ,zui di wei 10chong 。you sao miao dian jing he yuan zi li xian wei jing de jie guo ke zhi ,fu xi hei cha duo tang de chun hua zu fen zai biao guan jie gou 、biao mian ping zheng du fang mian jun ju you ming xian cha yi 。XRDyan she fen xi ke zhi fu xi hei cha duo tang cu pin he chun hua zu fen zai jing ti xing cheng fang mian cha yi xian zhe 。er DSCfen xi jie guo biao ming fu xi hei cha duo tang chun hua zu fen zhi jian re te xing bian hua jun ju you xian zhe cha yi 。(3)ABTS~+zi you ji qing chu shi yan biao ming ,fu xi hei cha duo tang cu pin ji ge chun hua zu fen dui ABTS~+zi you ji qing chu huo xing yu dui zhao zu xiang bi cha yi xian zhe ,dan ge zu fen zhi jian mo ming xian cha yi 。chao yang yin li zi zi you ji qing chu shi yan biao ming ,fu xi hei cha duo tang cu pin ji ge chun hua zu fen yu dui zhao zu xiang bi cha yi xian zhe ,dan ge zu fen zhi jian mo xian zhe cha yi 。α-pu tao tang gan mei yi zhi lv shi yan jie guo biao ming fu xi hei cha duo tang cu pin ji ge chun hua zu fen jun dui α-pu tao tang gan mei you xian zhe yi zhi huo xing bing ju he yang xing dui zhao a ka bo tang zu xiang bi cha yi bu xian zhe 。tong guo ren gong wei ye ti wai xiao hua shi yan ke zhi jing di qu chun hua huo de de fu xi hei cha duo tang bu yi bei wei ye xiao hua 。er jing ren gong chang ye de ti wai xiao hua shi yan ke zhi fu xi hei cha duo tang cu pin ji ji zu fen yi bu yi bei chang ye xiao hua 。(4)tong guo dui gao de lv 、gao ping zheng du zu fen 4jin yi bu fen li chun hua ,de dao le jun yi zu fen ,bing dui jun yi zu fen jin hang chao yang yin li zi zi you ji he ABTS~+zi you ji de qing chu lv fen xi 。jie guo biao ming zi you ji qing chu huo xing yu jun yi zu fen nong du cheng zheng xiang guan ,ju nong du zai 1mg/mlshi ABTSzi you ji qing chu lv da dao 80%,chao yang yin li zi zi you ji neng da dao 70%。
论文参考文献
论文详细介绍
论文作者分别是来自上海师范大学的张军耀，发表于刊物上海师范大学2019-06-17论文，是一篇关于富硒黑茶多糖论文,分离纯化论文,理化性质论文,体外活性论文,上海师范大学2019-06-17论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自上海师范大学2019-06-17论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：富硒黑茶多糖论文; 分离纯化论文; 理化性质论文; 体外活性论文; 上海师范大学2019-06-17论文;