本文主要研究内容
作者(2019)在《A rapid, simple, and sensitive immunoagglutination assay with silica nanoparticles for serotype identification of Pseudomonas aeruginosa》一文中研究指出:An agglutination test based on colored silica nanoparticles(colored SiNps) was established to detect serotypes of Pseudomonas aeruginosa. Monodisperse colored SiNps were used as agglutination test carriers. The colored SiNps were prepared through reverse microemulsion with reactive dyes, sensitized with 11 kinds of mono-specific antibodies against P. aeruginosa, and denoted as IgG-colored SiNps. Eleven kinds of IgG-colored SiNps were individually mixed with P. aeruginosa on a glass slide.Different serotypes of P. aeruginosa could be identified by agglutination test with evident agglutination. The P. aeruginosa could be detected in a range from 3.6×105 to 3.6×1012 cfu mL–1. This new agglutination test was confirmed to be a specific,sensitive, fast, easy-to-perform, and cost-efficient tool for the routine diagnosis of P. aeruginosa.
Abstract
An agglutination test based on colored silica nanoparticles(colored SiNps) was established to detect serotypes of Pseudomonas aeruginosa. Monodisperse colored SiNps were used as agglutination test carriers. The colored SiNps were prepared through reverse microemulsion with reactive dyes, sensitized with 11 kinds of mono-specific antibodies against P. aeruginosa, and denoted as IgG-colored SiNps. Eleven kinds of IgG-colored SiNps were individually mixed with P. aeruginosa on a glass slide.Different serotypes of P. aeruginosa could be identified by agglutination test with evident agglutination. The P. aeruginosa could be detected in a range from 3.6×105 to 3.6×1012 cfu mL–1. This new agglutination test was confirmed to be a specific,sensitive, fast, easy-to-perform, and cost-efficient tool for the routine diagnosis of P. aeruginosa.
论文参考文献
[1].A novel gene carrier based on amino-modified silica nanoparticles[J]. HE Xiaoxiao, WANG Kemin, TAN Weihong, LIU Bin, LIN Xia, HUANG Shasheng, LI Du, HE Chunmei & LI Jun State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry & Chemical Engineering, Institute of Biological Technol-ogy, Hunan University, Changsha 410082, China.  Chinese Science Bulletin.2003(03)[2].Transcriptional Factor Snail Mediates Epithelial-Mesenchymal Transition in Human Bronchial Epithelial Cells Induced by Silica[J]. HU Yong Bin,LI Fei Feng,DENG Zheng Hao,PAN Pin Hua.  Biomedical and Environmental Sciences.2015(07)[3].Fluorescent AIE dots encapsulated organically modified silica (ORMOSIL) nanoparticles for two-photon cellular imaging[J]. ZHU ZhenFeng,ZHAO XinYuan,QIN Wei,CHEN GuangDi,QIAN Jun,XU ZhengPing.  中国科学:化学.2013(09)[4].The biogenic silica composition, behavior and budget in the Changjiang Estuary[J]. RAN Xiangbin,LIU Jun,LIU Sen,ZANG Jiaye,WANG Baodong,ZHAO Jun.  Acta Oceanologica Sinica.2018(01)[5].Ordered SBA-15 mesoporous silica with high amino-functionalization for adsorption of heavy metal ions[J]. ZHANG Dan,LI JunHua.  Chinese Science Bulletin.2013(08)[6].Experimental study of miRNA200a regulating Wnt/β-catenin signaling pathway in silica-induced mouse lung epithelial cells[J]. 王欣.  China Medical Abstracts(Internal Medicine).2018(02)[7].Development of the model of pancreatic β-cell membrane chromatography and its chromatographic characteristics[J]. YANG Guangde, HE Langchong, BIAN Xiaoli & ZHAO Liang Department of Pharmacy, School of Medicine, Xi’an Jiaotong University, Xi’an 710061, China.  Chinese Science Bulletin.2005(21)[8].Effects of niacin on nitric oxide synthase expression in rat lungs exposed to silica[J]. WANG Shixin 1,2 *, DU Haike 2, ZHANG Xizhen 1, CAI Shaoxi 1, FAN Huaquan 3 and WANG Chang’en 4(1. College of Bioengineering, Chongqing University, Chongqing 400044, China; 2. Department of Science Research, Affiliated Hospital, Medical College of Chinese People’s Armed Police Forces, Tianjin 300162, China; 3. Third Military Medical University, Chongqing 400038, China; 4. Department of Life Sciences, National Natural Science Foundation of China, Beijing 100085, China).  Progress in Natural Science.2004(12)[9].CTAB-silica Method for DNA Extraction and Purification from Castanea mollissima and Ginkgo biloba[J]. Shen Yongbao* Shi Jisen National Southern Tree Seed Inspection Center, Nanjing Forestry University, Nanjing 210037, P.R.China.  Forestry Studies in China.2003(03)[10].Novel one-step synthesis of silica nanoparticles from sugarbeet bagasse by laser ablation and their effects on the growth of freshwater algae culture[J]. Nalan Oya San,Canan Kursung?z,Yasin Tümtas,?ncay Yasa,Bülend Orta?,Turgay Tekinay.  Particuology.2014(06)
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:A rapid, simple, and sensitive immunoagglutination assay with silica nanoparticles for serotype identification of Pseudomonas aeruginosa论文
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