导读:本文包含了转基因银腺杂种杨论文开题报告文献综述及选题提纲参考文献，主要关键词:透明颤菌血红蛋白基因(vgb),耐涝性,银腺杂种杨
转基因银腺杂种杨论文文献综述
李义良,苏晓华,张冰玉,黄秦军,张香华[1]（2009）在《转vgb基因银腺杂种杨的耐涝性》一文中研究指出对导入透明颤菌血红蛋白(vitreoscilla hemoglobin,VHb)基因vgb银腺杂种杨进行Southern印记杂交和RT-PCR检测,证实vgb基因已整合到杨树基因组中,并在转录水平上得到表达。在温室对4个转基因株系及对照盆栽扦插苗进行模拟持续淹水胁迫试验,研究淹水条件下植株的生长及生理性状。结果表明,随着淹水时间的延长苗木逐渐死亡,对照植株在淹水17天后死亡率达66.67%,而4个转基因株系死亡率均低于50.00%,其中T81最低,只有25.00%;同时4个转基因株系在淹水胁迫时能够保持较高的叶绿素含量,并且膜质过氧化物MDA含量均低于对照植株;涝渍胁迫下转基因株系生长受抑制程度低于对照植株。说明在淹水胁迫下vgb基因的表达增强了银腺杂种杨叶绿素含量的积累,进而促进了转基因植株的生长,提高了转基因株系对淹水的忍受能力。(本文来源于《林业科学》期刊2009年07期）
侯英杰,苏晓华,焦如珍,黄秦军,褚延广[2]（2009）在《转基因银腺杂种杨对土壤微生物的影响》一文中研究指出In this study,genetic stability of foreign genes was checked by using PCR technology. A total of 4 lines of soil samples from transgenic and non-transgenic control Populus alba×P.glandulosa and the soil samples from the stands were collected in two successive years,and the bacteria,fungi and antinomyces were isolated by using flat dilution method to determine their quantities. The results demonstrated that target genes were stable present in the genome of transgenic poplar. We found that three kinds of soil microorganism appeared different trends within different months and different years. The ANOVA and multiple comparison analyses revealed that there was no significant difference in quantity of the soil microorganisms among most poplar lines at the same time point. Meanwhile,significant differences of the soil microorganism community were found among several transgenic lines,and between a few transgenic and non-transgenic lines,but they did not show any relation to the type and number of foreign genes. Our preliminary results indicated that the 2-year-old transgenic poplar growing in field had no significant effects on soil microorganisms.(本文来源于《林业科学》期刊2009年05期）
转基因银腺杂种杨论文开题报告
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In this study,genetic stability of foreign genes was checked by using PCR technology. A total of 4 lines of soil samples from transgenic and non-transgenic control Populus alba×P.glandulosa and the soil samples from the stands were collected in two successive years,and the bacteria,fungi and antinomyces were isolated by using flat dilution method to determine their quantities. The results demonstrated that target genes were stable present in the genome of transgenic poplar. We found that three kinds of soil microorganism appeared different trends within different months and different years. The ANOVA and multiple comparison analyses revealed that there was no significant difference in quantity of the soil microorganisms among most poplar lines at the same time point. Meanwhile,significant differences of the soil microorganism community were found among several transgenic lines,and between a few transgenic and non-transgenic lines,but they did not show any relation to the type and number of foreign genes. Our preliminary results indicated that the 2-year-old transgenic poplar growing in field had no significant effects on soil microorganisms.
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转基因银腺杂种杨论文参考文献
[1].李义良,苏晓华,张冰玉,黄秦军,张香华.转vgb基因银腺杂种杨的耐涝性[J].林业科学.2009
[2].侯英杰,苏晓华,焦如珍,黄秦军,褚延广.转基因银腺杂种杨对土壤微生物的影响[J].林业科学.2009
标签：透明颤菌血红蛋白基因(vgb); 耐涝性; 银腺杂种杨;