本文主要研究内容
作者魏巧莉（2019）在《定时输精程序对后备母猪发情、配种效果及影响机制研究》一文中研究指出：定时输精技术在生产中可提高母畜利用率、降低非生产天数、建立批次化生产模式、提高生产效率,在规模化生产中发挥着重要作用。目前该技术在后备母猪的生产应用上仍存在诸多问题,如定时输精技术程序尚需优化、稳定性有待提高以及定时输精影响后备母猪繁殖性能的相关分子机制仍不清楚等。为优化定时输精技术程序,本试验开展了初情期有无、不同定时输精技术程序对后备母猪发情、排卵、妊娠、产仔效果的影响。接着,利用转录组测序技术对不同妊娠阶段母猪黄体组织繁殖相关基因的表达进行检测,并验证候选基因在不同妊娠阶段母猪黄体组织中的表达模式。为探讨初情期有无对后备母猪定时输精效果的影响,随机将182头有初情期后备母猪分为3组:对照组(C1组,62头)、烯丙孕素组(A1组,60头)、烯丙孕素+PMSG+GnRH组(APG1组,60头);193头无初情期后备母猪随机分为:对照组(C2组,63头)、烯丙孕素组(A2组,65头)、烯丙孕素+PMSG+GnRH组(APG2组,65头)。比较不同组间后备母猪的发情率和参繁母猪妊娠率。结果显示:无初情期组C2、A2、APG2发情率分别显著低于有情期C1、A1、APG1组(35.48±3.83%vs.23.81±3.33%,33.85±4.58%vs.63.33±4.92%,58.46±3.75%vs.83.33±3.79%)。对妊娠效果进行统计,经烯丙孕素处理后无初情期(A2)组妊娠率仅为29.23±4.36%,显著低于有初情期(A1)组(53.33±3.78%)(P<0.05);经烯丙孕素+PMSG+GnRH处理后,无初情期(APG2)组妊娠率亦显著低于有初情期(APG1)组(53.38±4.82%vs.83.33±4.26%)(P<0.01)。表明初情期是影响后备母猪定时输精效果的关键因素,在有情期后备母猪中使用定时输精程序效果更佳。对两种定时输精程序效果的研究表明:A1、A2、APG1、APG2组发情率(63.33±4.92%,33.85±4.58%,83.33±3.79%,58.46±3.75%)显著高于C1、C2组(35.48±3.83%,23.81±3.33%)(P<0.05),且APG1、APG2组发情率显著高于A1、A2组(P<0.05),表明两种定时输精程序均可显著提高后备母猪发情率,且烯丙孕素+PMSG+GnRH程序处理组在提高母猪发情率上效果更好。对妊娠效果的统计结果显示:与对照组C1、C2组(27.42±3.26%,19.05±3.76%)相比,A1、A2组(53.33±3.78%,29.23±4.36%)尽管也显著提高了后备母猪妊娠率,但低于APG1、APG2组(83.33±4.26%,55.38±4.82%),说明定时输精程序可显著提高后备母猪发情率与妊娠率,且以烯丙孕素+PMSG+GnRH程序处理效果更优。对发情集中度的统计结果表明:仅用烯丙孕素处理的A1组、A2组集中于停药后第5~9 d发情,且A2组存在明显发情延后现象;而烯丙孕素+PMSG+GnRH处理后APG1组和APG2组均集中于停喂烯丙孕素后第5 d和第6 d发情。表明烯丙孕素+PMSG+GnRH处理提高了后备母猪发情集中度。对窝产仔数、窝产活仔数的统计结果表明,APG1组(12.98±1.78,12.75±1.61)、A1组(12.43±2.46,12.03±2.82)显著高于C1组(10.98±4.52,10.02±2.86)(P<0.05),且APG1组高于A1组。说明两种定时输精程序均可有效提高母窝产仔数,且烯丙孕素+PMSG+GnRH程序处理组在提高窝产仔数上效果更好。综上可知,利用烯丙孕素+PMSG+GnRH程序在提高后备母猪繁殖性能方面效果更优。为探究定时输精对后备母猪生殖器官及早期胚胎发育的影响,随机选择经烯丙孕素+PMSG+GnRH处理(试验组)和未经任何处理的(对照组)12头妊娠后备母猪,于妊娠16天和25天屠宰,比较卵巢黄体数、子宫重量、子宫角长度、胚胎直径,并对卵巢黄体组织进行转录组测序。结果表明:试验组妊娠16天(16APG)和25天(25APG)卵巢总重量、卵巢总黄体数、子宫角测量总长度分别显著高于同时期对照组(16C与25C)后备母猪(P<0.01),且左右侧卵巢重量在各组间差异不显著(P>0.05),但左右侧卵巢黄体数、子宫角长度在试验组与对照组间差异显著(P<0.05),同时妊娠25天试验组子宫重量极显著高于对照组(P<0.01)。对胚胎直径测量结果显示,试验组妊娠16天胚胎长度与对照组差异不显著(P>0.05)、但妊娠25天试验组显著大于对照组(P<0.05)。对定时输精程序处理的后备母猪不同妊娠阶段黄体组织进行转录组测序,结果显示有30143个基因在妊娠的不同阶段及不同处理组间差异表达(P<0.05,差异倍数>0.0)。通过聚类分析,16APG组与16C组间差异基因数远远大于25APG组与25C组间的差异基因数(2394vs.1476)。16APG组与25APG组间差异基因数与16C组与25C组间差异基因数接近(1211vs.1060)。说明妊娠天数引起的基因差异在妊娠25天与妊娠16天时表达接近,暗示定时输精程序处理是引起妊娠不同阶段后备母猪基因差异变化的主要因素,且妊娠期卵巢黄体组织表达差异主要出现在附植初期。利用GO分析、聚类分析和KEGG分析筛选出差异表达基因:CYP17A1、IL18、LDLR、DCN、RPL10、PRLR。经qPCR验证其在黄体组织中的表达,结果显示RPL10、CYP17A1、DCN、IL18、LDLR在定时输精组表达显著高于对照组(P<0.05)。PRLR在定时输精组中的表达极显著低于对照组(P<0.01)。综上所述,定时输精处理应选择有初情期的后备母猪;烯丙孕素+PMSG+GnRH程序能更显著提高后备母猪繁殖效率;CYP17A1、LDLR、IL18、DCN、RPL10基因表达量升高,PRLR表达量降低很可能是烯丙孕素+PMSG+GnRH程序提高后备母猪母猪繁殖效率的重要原因。
Abstract
ding shi shu jing ji shu zai sheng chan zhong ke di gao mu chu li yong lv 、jiang di fei sheng chan tian shu 、jian li pi ci hua sheng chan mo shi 、di gao sheng chan xiao lv ,zai gui mo hua sheng chan zhong fa hui zhao chong yao zuo yong 。mu qian gai ji shu zai hou bei mu zhu de sheng chan ying yong shang reng cun zai zhu duo wen ti ,ru ding shi shu jing ji shu cheng xu shang xu you hua 、wen ding xing you dai di gao yi ji ding shi shu jing ying xiang hou bei mu zhu fan shi xing neng de xiang guan fen zi ji zhi reng bu qing chu deng 。wei you hua ding shi shu jing ji shu cheng xu ,ben shi yan kai zhan le chu qing ji you mo 、bu tong ding shi shu jing ji shu cheng xu dui hou bei mu zhu fa qing 、pai luan 、ren shen 、chan zai xiao guo de ying xiang 。jie zhao ,li yong zhuai lu zu ce xu ji shu dui bu tong ren shen jie duan mu zhu huang ti zu zhi fan shi xiang guan ji yin de biao da jin hang jian ce ,bing yan zheng hou shua ji yin zai bu tong ren shen jie duan mu zhu huang ti zu zhi zhong de biao da mo shi 。wei tan tao chu qing ji you mo dui hou bei mu zhu ding shi shu jing xiao guo de ying xiang ,sui ji jiang 182tou you chu qing ji hou bei mu zhu fen wei 3zu :dui zhao zu (C1zu ,62tou )、xi bing yun su zu (A1zu ,60tou )、xi bing yun su +PMSG+GnRHzu (APG1zu ,60tou );193tou mo chu qing ji hou bei mu zhu sui ji fen wei :dui zhao zu (C2zu ,63tou )、xi bing yun su zu (A2zu ,65tou )、xi bing yun su +PMSG+GnRHzu (APG2zu ,65tou )。bi jiao bu tong zu jian hou bei mu zhu de fa qing lv he can fan mu zhu ren shen lv 。jie guo xian shi :mo chu qing ji zu C2、A2、APG2fa qing lv fen bie xian zhe di yu you qing ji C1、A1、APG1zu (35.48±3.83%vs.23.81±3.33%,33.85±4.58%vs.63.33±4.92%,58.46±3.75%vs.83.33±3.79%)。dui ren shen xiao guo jin hang tong ji ,jing xi bing yun su chu li hou mo chu qing ji (A2)zu ren shen lv jin wei 29.23±4.36%,xian zhe di yu you chu qing ji (A1)zu (53.33±3.78%)(P<0.05);jing xi bing yun su +PMSG+GnRHchu li hou ,mo chu qing ji (APG2)zu ren shen lv yi xian zhe di yu you chu qing ji (APG1)zu (53.38±4.82%vs.83.33±4.26%)(P<0.01)。biao ming chu qing ji shi ying xiang hou bei mu zhu ding shi shu jing xiao guo de guan jian yin su ,zai you qing ji hou bei mu zhu zhong shi yong ding shi shu jing cheng xu xiao guo geng jia 。dui liang chong ding shi shu jing cheng xu xiao guo de yan jiu biao ming :A1、A2、APG1、APG2zu fa qing lv (63.33±4.92%,33.85±4.58%,83.33±3.79%,58.46±3.75%)xian zhe gao yu C1、C2zu (35.48±3.83%,23.81±3.33%)(P<0.05),ju APG1、APG2zu fa qing lv xian zhe gao yu A1、A2zu (P<0.05),biao ming liang chong ding shi shu jing cheng xu jun ke xian zhe di gao hou bei mu zhu fa qing lv ,ju xi bing yun su +PMSG+GnRHcheng xu chu li zu zai di gao mu zhu fa qing lv shang xiao guo geng hao 。dui ren shen xiao guo de tong ji jie guo xian shi :yu dui zhao zu C1、C2zu (27.42±3.26%,19.05±3.76%)xiang bi ,A1、A2zu (53.33±3.78%,29.23±4.36%)jin guan ye xian zhe di gao le hou bei mu zhu ren shen lv ,dan di yu APG1、APG2zu (83.33±4.26%,55.38±4.82%),shui ming ding shi shu jing cheng xu ke xian zhe di gao hou bei mu zhu fa qing lv yu ren shen lv ,ju yi xi bing yun su +PMSG+GnRHcheng xu chu li xiao guo geng you 。dui fa qing ji zhong du de tong ji jie guo biao ming :jin yong xi bing yun su chu li de A1zu 、A2zu ji zhong yu ting yao hou di 5~9 dfa qing ,ju A2zu cun zai ming xian fa qing yan hou xian xiang ;er xi bing yun su +PMSG+GnRHchu li hou APG1zu he APG2zu jun ji zhong yu ting wei xi bing yun su hou di 5 dhe di 6 dfa qing 。biao ming xi bing yun su +PMSG+GnRHchu li di gao le hou bei mu zhu fa qing ji zhong du 。dui wo chan zai shu 、wo chan huo zai shu de tong ji jie guo biao ming ,APG1zu (12.98±1.78,12.75±1.61)、A1zu (12.43±2.46,12.03±2.82)xian zhe gao yu C1zu (10.98±4.52,10.02±2.86)(P<0.05),ju APG1zu gao yu A1zu 。shui ming liang chong ding shi shu jing cheng xu jun ke you xiao di gao mu wo chan zai shu ,ju xi bing yun su +PMSG+GnRHcheng xu chu li zu zai di gao wo chan zai shu shang xiao guo geng hao 。zeng shang ke zhi ,li yong xi bing yun su +PMSG+GnRHcheng xu zai di gao hou bei mu zhu fan shi xing neng fang mian xiao guo geng you 。wei tan jiu ding shi shu jing dui hou bei mu zhu sheng shi qi guan ji zao ji pei tai fa yo de ying xiang ,sui ji shua ze jing xi bing yun su +PMSG+GnRHchu li (shi yan zu )he wei jing ren he chu li de (dui zhao zu )12tou ren shen hou bei mu zhu ,yu ren shen 16tian he 25tian tu zai ,bi jiao luan chao huang ti shu 、zi gong chong liang 、zi gong jiao chang du 、pei tai zhi jing ,bing dui luan chao huang ti zu zhi jin hang zhuai lu zu ce xu 。jie guo biao ming :shi yan zu ren shen 16tian (16APG)he 25tian (25APG)luan chao zong chong liang 、luan chao zong huang ti shu 、zi gong jiao ce liang zong chang du fen bie xian zhe gao yu tong shi ji dui zhao zu (16Cyu 25C)hou bei mu zhu (P<0.01),ju zuo you ce luan chao chong liang zai ge zu jian cha yi bu xian zhe (P>0.05),dan zuo you ce luan chao huang ti shu 、zi gong jiao chang du zai shi yan zu yu dui zhao zu jian cha yi xian zhe (P<0.05),tong shi ren shen 25tian shi yan zu zi gong chong liang ji xian zhe gao yu dui zhao zu (P<0.01)。dui pei tai zhi jing ce liang jie guo xian shi ,shi yan zu ren shen 16tian pei tai chang du yu dui zhao zu cha yi bu xian zhe (P>0.05)、dan ren shen 25tian shi yan zu xian zhe da yu dui zhao zu (P<0.05)。dui ding shi shu jing cheng xu chu li de hou bei mu zhu bu tong ren shen jie duan huang ti zu zhi jin hang zhuai lu zu ce xu ,jie guo xian shi you 30143ge ji yin zai ren shen de bu tong jie duan ji bu tong chu li zu jian cha yi biao da (P<0.05,cha yi bei shu >0.0)。tong guo ju lei fen xi ,16APGzu yu 16Czu jian cha yi ji yin shu yuan yuan da yu 25APGzu yu 25Czu jian de cha yi ji yin shu (2394vs.1476)。16APGzu yu 25APGzu jian cha yi ji yin shu yu 16Czu yu 25Czu jian cha yi ji yin shu jie jin (1211vs.1060)。shui ming ren shen tian shu yin qi de ji yin cha yi zai ren shen 25tian yu ren shen 16tian shi biao da jie jin ,an shi ding shi shu jing cheng xu chu li shi yin qi ren shen bu tong jie duan hou bei mu zhu ji yin cha yi bian hua de zhu yao yin su ,ju ren shen ji luan chao huang ti zu zhi biao da cha yi zhu yao chu xian zai fu zhi chu ji 。li yong GOfen xi 、ju lei fen xi he KEGGfen xi shai shua chu cha yi biao da ji yin :CYP17A1、IL18、LDLR、DCN、RPL10、PRLR。jing qPCRyan zheng ji zai huang ti zu zhi zhong de biao da ,jie guo xian shi RPL10、CYP17A1、DCN、IL18、LDLRzai ding shi shu jing zu biao da xian zhe gao yu dui zhao zu (P<0.05)。PRLRzai ding shi shu jing zu zhong de biao da ji xian zhe di yu dui zhao zu (P<0.01)。zeng shang suo shu ,ding shi shu jing chu li ying shua ze you chu qing ji de hou bei mu zhu ;xi bing yun su +PMSG+GnRHcheng xu neng geng xian zhe di gao hou bei mu zhu fan shi xiao lv ;CYP17A1、LDLR、IL18、DCN、RPL10ji yin biao da liang sheng gao ,PRLRbiao da liang jiang di hen ke neng shi xi bing yun su +PMSG+GnRHcheng xu di gao hou bei mu zhu mu zhu fan shi xiao lv de chong yao yuan yin 。
论文参考文献
论文详细介绍
论文作者分别是来自河北农业大学的魏巧莉，发表于刊物河北农业大学2019-09-19论文，是一篇关于定时输精论文,发情论文,妊娠论文,转录组论文,后备母猪论文,河北农业大学2019-09-19论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自河北农业大学2019-09-19论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。