本文主要研究内容
作者（2019）在《Comparative expression analysis of heavy metal ATPase subfamily genes between Cd-tolerant and Cd-sensitive turnip landraces》一文中研究指出：The heavy metal ATPase(HMA) subfamily is mainly involved in heavy metal(HM) tolerance and transport in plants, but an understanding of the definite roles and mechanisms of most HMA members are still limited. In the present study, we identified 14 candidate HMA genes named Brr HMA1 e Brr HMA8 from the turnip genome and analyzed the phylogeny, gene structure, chromosome distribution, and conserved domains and motifs of HMAs in turnip(Brassica rapa var. rapa). According to our phylogenetic tree, the Brr HMAs are divided into a Zn/Cd/Co/Pb subclass and Cu/Ag subclass. The Brr HMA members show similar structural characteristics within subclasses. To explore the roles of Brr HMAs in turnip, we compared the gene sequences and expression patterns of the Brr HMA genes between a Cd-tolerant landrace and a Cd-sensitive landrace. Most Brr HMA genes showed similar spatial expression patterns in both Cd-tolerant and Cd-sensitive turnip landraces; some Brr HMA genes, however, were differentially expressed in specific tissue in Cd-tolerant and Cd-sensitive turnip.Specifically, Brr HMA genes in the Zn/Cd/Co/Pb subclass shared the same coding sequence but were differentially expressed in Cd-tolerant and Cd-sensitive turnip landraces under Cd stress. Our findings suggest that the stable expression and up-regulated expression of Brr HMA Zn/Cd/Co/Pb subclass genes under Cd stress may contribute to the higher Cd tolerance of turnip landraces.
Abstract
The heavy metal ATPase(HMA) subfamily is mainly involved in heavy metal(HM) tolerance and transport in plants, but an understanding of the definite roles and mechanisms of most HMA members are still limited. In the present study, we identified 14 candidate HMA genes named Brr HMA1 e Brr HMA8 from the turnip genome and analyzed the phylogeny, gene structure, chromosome distribution, and conserved domains and motifs of HMAs in turnip(Brassica rapa var. rapa). According to our phylogenetic tree, the Brr HMAs are divided into a Zn/Cd/Co/Pb subclass and Cu/Ag subclass. The Brr HMA members show similar structural characteristics within subclasses. To explore the roles of Brr HMAs in turnip, we compared the gene sequences and expression patterns of the Brr HMA genes between a Cd-tolerant landrace and a Cd-sensitive landrace. Most Brr HMA genes showed similar spatial expression patterns in both Cd-tolerant and Cd-sensitive turnip landraces; some Brr HMA genes, however, were differentially expressed in specific tissue in Cd-tolerant and Cd-sensitive turnip.Specifically, Brr HMA genes in the Zn/Cd/Co/Pb subclass shared the same coding sequence but were differentially expressed in Cd-tolerant and Cd-sensitive turnip landraces under Cd stress. Our findings suggest that the stable expression and up-regulated expression of Brr HMA Zn/Cd/Co/Pb subclass genes under Cd stress may contribute to the higher Cd tolerance of turnip landraces.
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