本文主要研究内容
作者吉果（2019）在《鸭疫里默氏杆菌RA-GD株RecA基因的功能研究》一文中研究指出：鸭疫里默氏杆菌(Riemerella anatipestifer)是一种无鞭毛、无芽孢,瑞氏染色呈两极着色的革兰氏阴性菌。该菌感染家鸭后,引起的传染病称为鸭传染性浆膜炎,以纤维素性心包炎等广泛性纤维素性炎症为临床病理特征。该病在世界各地广泛分布,具有较高的发病率和死亡率,是造成养鸭业重大损失的主要传染病之一。细菌DNA损伤诱导反应又称为SOS反应,是指细菌应对基因组DNA损伤的重要保护机制。RecA/LexA蛋白在很多细菌的SOS反应诱导中起关键调控作用。研究清楚RecA基因的功能及其可能在DNA损伤修复反应(SOS应答)中发挥的作用,有助于理解减弱细菌毒力和进化,降低细菌致病性,同时有助于降低并控制日益严峻的细菌耐药性,以及为开发新型抗菌小分子及生物制剂,减少化学药物的使用以控制病原菌方面发挥着一定作用。首先在同源重组的基础上,通过改造后的自杀性质粒PRE112-RecA-Erm构建缺失株RA-GD?RecA。在缺失株的基础上,通过对回复质粒PCP29逐步引入多克隆位点(Mcs)、复制区(Ori)、启动子(Psr)和RecA基因序列,构建成功回复质粒Pcp29-Mcs-Ori-Psr-RecA,与亲本株RA-GD通过接合转移构建回复株cRA-GD?RecA。其次,对上述3种鸭疫里默氏杆菌(亲本株RA-GD、缺失株RA-GD?RecA和回补株cRA-GD?RecA)分别开展生长曲线、药敏试验、雏鸭毒力试验等生物学特性研究。结果表明,RecA基因并不影响鸭疫里默氏杆菌RA-GD株的生长速率;RecA基因在降低鸭疫里默氏杆菌对硫酸庆大霉素的敏感性方面发挥作用;与RA-GD相比,RecA基因的缺失显著降低了RA-GD?RecA对雏鸭的致死率,表明RecA基因可能参与影响并减弱了鸭疫里默氏杆菌毒力因子的表达。最后,比较亲本株RA-GD和回复株cRA-GD?RecA在UV损伤前后,细菌存活数、RecA基因的转录和蛋白表达水平。结果表明,在一定限度内,RecA基因的转录水平随UV辐照时间的延长而增加。在UV引起的DNA损伤试验中,UV因素可以引发细菌发生DNA损伤应答(SOS应答),表明RecA基因可能在鸭疫里默氏杆菌RA-GD株的SOS应答中发挥正调控作用。亲本株RA-GD与回复株cRA-GD?RecA的Western blotting证实,RecA蛋白反应原性良好,但UV因素并不参与影响RecA蛋白水平的表达。
Abstract
ya yi li mo shi gan jun (Riemerella anatipestifer)shi yi chong mo bian mao 、mo ya bao ,rui shi ran se cheng liang ji zhao se de ge lan shi yin xing jun 。gai jun gan ran jia ya hou ,yin qi de chuan ran bing chen wei ya chuan ran xing jiang mo yan ,yi qian wei su xing xin bao yan deng an fan xing qian wei su xing yan zheng wei lin chuang bing li te zheng 。gai bing zai shi jie ge de an fan fen bu ,ju you jiao gao de fa bing lv he si wang lv ,shi zao cheng yang ya ye chong da sun shi de zhu yao chuan ran bing zhi yi 。xi jun DNAsun shang you dao fan ying you chen wei SOSfan ying ,shi zhi xi jun ying dui ji yin zu DNAsun shang de chong yao bao hu ji zhi 。RecA/LexAdan bai zai hen duo xi jun de SOSfan ying you dao zhong qi guan jian diao kong zuo yong 。yan jiu qing chu RecAji yin de gong neng ji ji ke neng zai DNAsun shang xiu fu fan ying (SOSying da )zhong fa hui de zuo yong ,you zhu yu li jie jian ruo xi jun du li he jin hua ,jiang di xi jun zhi bing xing ,tong shi you zhu yu jiang di bing kong zhi ri yi yan jun de xi jun nai yao xing ,yi ji wei kai fa xin xing kang jun xiao fen zi ji sheng wu zhi ji ,jian shao hua xue yao wu de shi yong yi kong zhi bing yuan jun fang mian fa hui zhao yi ding zuo yong 。shou xian zai tong yuan chong zu de ji chu shang ,tong guo gai zao hou de zi sha xing zhi li PRE112-RecA-Ermgou jian que shi zhu RA-GD?RecA。zai que shi zhu de ji chu shang ,tong guo dui hui fu zhi li PCP29zhu bu yin ru duo ke long wei dian (Mcs)、fu zhi ou (Ori)、qi dong zi (Psr)he RecAji yin xu lie ,gou jian cheng gong hui fu zhi li Pcp29-Mcs-Ori-Psr-RecA,yu qin ben zhu RA-GDtong guo jie ge zhuai yi gou jian hui fu zhu cRA-GD?RecA。ji ci ,dui shang shu 3chong ya yi li mo shi gan jun (qin ben zhu RA-GD、que shi zhu RA-GD?RecAhe hui bu zhu cRA-GD?RecA)fen bie kai zhan sheng chang qu xian 、yao min shi yan 、chu ya du li shi yan deng sheng wu xue te xing yan jiu 。jie guo biao ming ,RecAji yin bing bu ying xiang ya yi li mo shi gan jun RA-GDzhu de sheng chang su lv ;RecAji yin zai jiang di ya yi li mo shi gan jun dui liu suan qing da mei su de min gan xing fang mian fa hui zuo yong ;yu RA-GDxiang bi ,RecAji yin de que shi xian zhe jiang di le RA-GD?RecAdui chu ya de zhi si lv ,biao ming RecAji yin ke neng can yu ying xiang bing jian ruo le ya yi li mo shi gan jun du li yin zi de biao da 。zui hou ,bi jiao qin ben zhu RA-GDhe hui fu zhu cRA-GD?RecAzai UVsun shang qian hou ,xi jun cun huo shu 、RecAji yin de zhuai lu he dan bai biao da shui ping 。jie guo biao ming ,zai yi ding xian du nei ,RecAji yin de zhuai lu shui ping sui UVfu zhao shi jian de yan chang er zeng jia 。zai UVyin qi de DNAsun shang shi yan zhong ,UVyin su ke yi yin fa xi jun fa sheng DNAsun shang ying da (SOSying da ),biao ming RecAji yin ke neng zai ya yi li mo shi gan jun RA-GDzhu de SOSying da zhong fa hui zheng diao kong zuo yong 。qin ben zhu RA-GDyu hui fu zhu cRA-GD?RecAde Western blottingzheng shi ,RecAdan bai fan ying yuan xing liang hao ,dan UVyin su bing bu can yu ying xiang RecAdan bai shui ping de biao da 。
论文参考文献
论文详细介绍
论文作者分别是来自中国农业科学院的吉果，发表于刊物中国农业科学院2019-07-05论文，是一篇关于鸭疫里默氏杆菌论文,基因论文,应答论文,损伤论文,中国农业科学院2019-07-05论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自中国农业科学院2019-07-05论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：鸭疫里默氏杆菌论文; 基因论文; 应答论文; 损伤论文; 中国农业科学院2019-07-05论文;