本文主要研究内容
作者徐传飞（2019）在《犏牛精子发生阻滞相关microRNA鉴定与功能分析》一文中研究指出：犏牛是雄性普通牛和雌性牦牛的种间F1代杂种,生产性能显著优于亲本,对青藏高原恶劣气候环境也具有杰出的适应能力,但雄性犏牛因精子发生阻滞不育极大的限制了其在牦牛杂交育种中的有效利用。miRNA作为一类非编码小RNA在转录后水平通过影响靶mRNA的稳定性或者抑制蛋白翻译过程从而调控基因的表达水平。为了解miRNA在犏牛精子发生阻滞中可能的调控作用,本研究通过illumina深度测序筛选鉴定牦牛及犏牛睾丸组织间差异表达miRNA;并初步分析了bta-miR-34c调控牦牛精原细胞分化的分子机制。结果如下:(1)通过牦牛与犏牛睾丸组织TUNEL-POD染色分析发现,犏牛睾丸组织生精小管内只有单层附着在生精小管基底膜的精原细胞以及少量精母细胞,无圆形或延伸的精子细胞,并且精原细胞最早开始发生凋亡,至精母细胞时期加重,从而导致后续精子发生中断以致无精子生成;(2)通过牦牛与犏牛睾丸组织转录组miRNA高通量测序以及生物信息学分析发现,61个miRNA在犏牛与牦牛睾丸组织之间差异表达显著,其中miR-19b-3p、miR-592、miR-135a、miR-378和miR-184参与SSCs自我更新以及分化过程;miR-34c和miR-449a参与精子发生减数分离起始过程;(3)通过STA-PUT自然沉降法分离得到纯度较高的牦牛精原细胞和精母细胞;RT-PCR表型鉴定发现牦牛精原细胞呈CD9以及UCHL1阳性,精母细胞呈Tesmin以及SYCP3阳性;(4)通过分别转染牦牛精原细胞bta-miR-34c mimic以及inhibitor后发现,bta-miR-34c可能通过抑制CD4以及CDC25A的活性降低E2F转录因子的表达,从而促进精原细胞由有丝分裂向减数分裂转变的分化过程;同时还可能通过抑制HDAC1以及SIRT1的表达以激活p53通路诱导生殖细胞凋亡,以维持生精小管内生精细胞数目稳态。
Abstract
pian niu shi xiong xing pu tong niu he ci xing mao niu de chong jian F1dai za chong ,sheng chan xing neng xian zhe you yu qin ben ,dui qing cang gao yuan e lie qi hou huan jing ye ju you jie chu de kuo ying neng li ,dan xiong xing pian niu yin jing zi fa sheng zu zhi bu yo ji da de xian zhi le ji zai mao niu za jiao yo chong zhong de you xiao li yong 。miRNAzuo wei yi lei fei bian ma xiao RNAzai zhuai lu hou shui ping tong guo ying xiang ba mRNAde wen ding xing huo zhe yi zhi dan bai fan yi guo cheng cong er diao kong ji yin de biao da shui ping 。wei le jie miRNAzai pian niu jing zi fa sheng zu zhi zhong ke neng de diao kong zuo yong ,ben yan jiu tong guo illuminashen du ce xu shai shua jian ding mao niu ji pian niu gao wan zu zhi jian cha yi biao da miRNA;bing chu bu fen xi le bta-miR-34cdiao kong mao niu jing yuan xi bao fen hua de fen zi ji zhi 。jie guo ru xia :(1)tong guo mao niu yu pian niu gao wan zu zhi TUNEL-PODran se fen xi fa xian ,pian niu gao wan zu zhi sheng jing xiao guan nei zhi you chan ceng fu zhao zai sheng jing xiao guan ji de mo de jing yuan xi bao yi ji shao liang jing mu xi bao ,mo yuan xing huo yan shen de jing zi xi bao ,bing ju jing yuan xi bao zui zao kai shi fa sheng diao wang ,zhi jing mu xi bao shi ji jia chong ,cong er dao zhi hou xu jing zi fa sheng zhong duan yi zhi mo jing zi sheng cheng ;(2)tong guo mao niu yu pian niu gao wan zu zhi zhuai lu zu miRNAgao tong liang ce xu yi ji sheng wu xin xi xue fen xi fa xian ,61ge miRNAzai pian niu yu mao niu gao wan zu zhi zhi jian cha yi biao da xian zhe ,ji zhong miR-19b-3p、miR-592、miR-135a、miR-378he miR-184can yu SSCszi wo geng xin yi ji fen hua guo cheng ;miR-34che miR-449acan yu jing zi fa sheng jian shu fen li qi shi guo cheng ;(3)tong guo STA-PUTzi ran chen jiang fa fen li de dao chun du jiao gao de mao niu jing yuan xi bao he jing mu xi bao ;RT-PCRbiao xing jian ding fa xian mao niu jing yuan xi bao cheng CD9yi ji UCHL1yang xing ,jing mu xi bao cheng Tesminyi ji SYCP3yang xing ;(4)tong guo fen bie zhuai ran mao niu jing yuan xi bao bta-miR-34c mimicyi ji inhibitorhou fa xian ,bta-miR-34cke neng tong guo yi zhi CD4yi ji CDC25Ade huo xing jiang di E2Fzhuai lu yin zi de biao da ,cong er cu jin jing yuan xi bao you you si fen lie xiang jian shu fen lie zhuai bian de fen hua guo cheng ;tong shi hai ke neng tong guo yi zhi HDAC1yi ji SIRT1de biao da yi ji huo p53tong lu you dao sheng shi xi bao diao wang ,yi wei chi sheng jing xiao guan nei sheng jing xi bao shu mu wen tai 。
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论文详细介绍
论文作者分别是来自西南科技大学的徐传飞，发表于刊物西南科技大学2019-09-02论文，是一篇关于犏牛论文,雄性不育论文,西南科技大学2019-09-02论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自西南科技大学2019-09-02论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：犏牛论文; 雄性不育论文; 西南科技大学2019-09-02论文;