本文主要研究内容
作者梁峰豪（2019）在《油菜抗根肿病种质的创制及相关抗病基因的定位分析》一文中研究指出：根肿病是由芸薹根肿菌（Plasmodiophora brassica Wor.）引起的土传性真菌病害。目前已经在世界60多个国家都有发生,每年对十字花科作物平均造成10%-15%的减产,致使根肿病成为制约十字花科作物生产的主要因素之一。甘蓝型油菜（Brassica napus L.）是世界上种植面积最广的油料作物,也是我国目前主要种植的油菜类型,具有产量高、抗病虫、抗逆性强等优点,但是甘蓝型油菜又大多不抗根肿病,造成根肿病在我国的发病面积逐年增加,成为我国油菜生产的主要病害。目前研究者认为,培育抗根肿病的品种并加以推广是解决油菜根肿病的最有效的途径,所以筛选抗根肿病种质资源、合成抗病种质、挖掘抗病基因就显得尤为重要。本研究主要从抗根肿病材料的筛选、根肿病相关抗性生理生化指标的分析、抗根肿病种质资源的创制以及相关抗病基因的定位四个方向研究。1.前期用来自四川成都的根肿菌4号生理小种,采用灌根法对40份芸薹属供试材料进行抗性鉴定,并对供试材料进行抗性显著性分析、病情指数分析、发病率与病情指数相关性分析,结果发现不同种质之间抗病性呈显著性差异;聚类分析将40份材料划分为高抗、中感、感病、高感4个抗性类型;发病率与病情指数两者之间具有显著相关性（P<0.05）。最终筛选出了10份高抗材料,包括3份白菜、1份甘蓝、3份欧洲芜菁、1份芥菜型油菜、1份萝卜、1份埃塞俄比亚芥,这为后续工作提供基础材料。2.以前期筛选的19份感病甘蓝型油菜为试验材料,将未接菌作为对照,从与抗病性相关的相对含水量、丙二醛含量、可溶性糖以及细胞膜相对透性四个生理指标进行分析。结果发现相对含水量、丙二醛含量及可溶性糖在接菌后未表现出明显差异,而植株在根肿菌胁迫下,细胞膜相对透性上升,并表现为显著性差异。所以,可以将细胞膜的相对透性做为前期筛选抗根肿病的生理辅助指标。3.以高感的甘蓝型油菜骨干亲本Xu09作为母本,以抗病的Debra（欧洲芜菁）为父本,通过远缘杂交获得了3颗杂交种子。通过形态学鉴定、SSR分子标记鉴定及抗性接种鉴定三种鉴定法对远缘杂交获得的种子进行真假杂种鉴定,结果显示所获得3颗种子均为真杂种,它们将会作为根肿病抗性育种的过渡“桥梁材料”。4.以甘蓝型油菜抗病不育系为母本与甘蓝型油菜感病恢复系作杂交,正常田间管理,成熟后获得杂种F1代。对回交转育获得的F1用根肿菌4号生理小种进行抗性鉴定,结果抗性分级整体为感病,抗感分离比约为2:1。对获得抗性单株,继续授以甘蓝型油菜花粉,获得BC1群体,用以后续研究。5.通过搜集查找已发表的与白菜抗根肿病基因紧密连锁的10个分子标记,对前期本实验室和他人筛选的15份抗感材料进行PCR扩增,初步筛选抗性材料中抗性基因。结果显示可能含有CRa、CRb、CRc、CRk、Crr1、Crr2和Crr3基因的材料分别有15份、3份、4份、4份、6份、5份和14份。其中白菜根抗518在CRa、CRb、CRc、Crr1、Crr2和Crr3位点均能扩增出多态性条带。6.以芥菜型油菜陕北黄芥（感病）与CT19（抗病）以及产生的F2代为试验材料,根据前期预测的基因,共设计24对IP引物,最终获得的5个有差异IP引物。利用其中的多态性的IP引物IP09-4对29份F2群体扫描,结果发现部分抗性标记扫描结果不能与抗性鉴定结果相对应。这说明控制该群体抗根肿病的主效基因可能不在这区域内。7.对70份芥菜型油菜陕北黄芥与CT19的F2群体（41份抗病、29份感病）采用简化基因组测序方法对根肿病抗性基因进行定位。根据获得的SNP/InDel标记的信息,计算不同基因型间的频率差异,预测基因所在区域。结果显示在A03染色体的29.3-40.7Mb区间存在一个显著QTL位点,该区域将会作为本研究的候选区域。
Abstract
gen zhong bing shi you yun tai gen zhong jun （Plasmodiophora brassica Wor.）yin qi de tu chuan xing zhen jun bing hai 。mu qian yi jing zai shi jie 60duo ge guo jia dou you fa sheng ,mei nian dui shi zi hua ke zuo wu ping jun zao cheng 10%-15%de jian chan ,zhi shi gen zhong bing cheng wei zhi yao shi zi hua ke zuo wu sheng chan de zhu yao yin su zhi yi 。gan lan xing you cai （Brassica napus L.）shi shi jie shang chong zhi mian ji zui an de you liao zuo wu ,ye shi wo guo mu qian zhu yao chong zhi de you cai lei xing ,ju you chan liang gao 、kang bing chong 、kang ni xing jiang deng you dian ,dan shi gan lan xing you cai you da duo bu kang gen zhong bing ,zao cheng gen zhong bing zai wo guo de fa bing mian ji zhu nian zeng jia ,cheng wei wo guo you cai sheng chan de zhu yao bing hai 。mu qian yan jiu zhe ren wei ,pei yo kang gen zhong bing de pin chong bing jia yi tui an shi jie jue you cai gen zhong bing de zui you xiao de tu jing ,suo yi shai shua kang gen zhong bing chong zhi zi yuan 、ge cheng kang bing chong zhi 、wa jue kang bing ji yin jiu xian de you wei chong yao 。ben yan jiu zhu yao cong kang gen zhong bing cai liao de shai shua 、gen zhong bing xiang guan kang xing sheng li sheng hua zhi biao de fen xi 、kang gen zhong bing chong zhi zi yuan de chuang zhi yi ji xiang guan kang bing ji yin de ding wei si ge fang xiang yan jiu 。1.qian ji yong lai zi si chuan cheng dou de gen zhong jun 4hao sheng li xiao chong ,cai yong guan gen fa dui 40fen yun tai shu gong shi cai liao jin hang kang xing jian ding ,bing dui gong shi cai liao jin hang kang xing xian zhe xing fen xi 、bing qing zhi shu fen xi 、fa bing lv yu bing qing zhi shu xiang guan xing fen xi ,jie guo fa xian bu tong chong zhi zhi jian kang bing xing cheng xian zhe xing cha yi ;ju lei fen xi jiang 40fen cai liao hua fen wei gao kang 、zhong gan 、gan bing 、gao gan 4ge kang xing lei xing ;fa bing lv yu bing qing zhi shu liang zhe zhi jian ju you xian zhe xiang guan xing （P<0.05）。zui zhong shai shua chu le 10fen gao kang cai liao ,bao gua 3fen bai cai 、1fen gan lan 、3fen ou zhou wu jing 、1fen gai cai xing you cai 、1fen luo bo 、1fen ai sai e bi ya gai ,zhe wei hou xu gong zuo di gong ji chu cai liao 。2.yi qian ji shai shua de 19fen gan bing gan lan xing you cai wei shi yan cai liao ,jiang wei jie jun zuo wei dui zhao ,cong yu kang bing xing xiang guan de xiang dui han shui liang 、bing er quan han liang 、ke rong xing tang yi ji xi bao mo xiang dui tou xing si ge sheng li zhi biao jin hang fen xi 。jie guo fa xian xiang dui han shui liang 、bing er quan han liang ji ke rong xing tang zai jie jun hou wei biao xian chu ming xian cha yi ,er zhi zhu zai gen zhong jun xie pai xia ,xi bao mo xiang dui tou xing shang sheng ,bing biao xian wei xian zhe xing cha yi 。suo yi ,ke yi jiang xi bao mo de xiang dui tou xing zuo wei qian ji shai shua kang gen zhong bing de sheng li fu zhu zhi biao 。3.yi gao gan de gan lan xing you cai gu gan qin ben Xu09zuo wei mu ben ,yi kang bing de Debra（ou zhou wu jing ）wei fu ben ,tong guo yuan yuan za jiao huo de le 3ke za jiao chong zi 。tong guo xing tai xue jian ding 、SSRfen zi biao ji jian ding ji kang xing jie chong jian ding san chong jian ding fa dui yuan yuan za jiao huo de de chong zi jin hang zhen jia za chong jian ding ,jie guo xian shi suo huo de 3ke chong zi jun wei zhen za chong ,ta men jiang hui zuo wei gen zhong bing kang xing yo chong de guo du “qiao liang cai liao ”。4.yi gan lan xing you cai kang bing bu yo ji wei mu ben yu gan lan xing you cai gan bing hui fu ji zuo za jiao ,zheng chang tian jian guan li ,cheng shou hou huo de za chong F1dai 。dui hui jiao zhuai yo huo de de F1yong gen zhong jun 4hao sheng li xiao chong jin hang kang xing jian ding ,jie guo kang xing fen ji zheng ti wei gan bing ,kang gan fen li bi yao wei 2:1。dui huo de kang xing chan zhu ,ji xu shou yi gan lan xing you cai hua fen ,huo de BC1qun ti ,yong yi hou xu yan jiu 。5.tong guo sou ji cha zhao yi fa biao de yu bai cai kang gen zhong bing ji yin jin mi lian suo de 10ge fen zi biao ji ,dui qian ji ben shi yan shi he ta ren shai shua de 15fen kang gan cai liao jin hang PCRkuo zeng ,chu bu shai shua kang xing cai liao zhong kang xing ji yin 。jie guo xian shi ke neng han you CRa、CRb、CRc、CRk、Crr1、Crr2he Crr3ji yin de cai liao fen bie you 15fen 、3fen 、4fen 、4fen 、6fen 、5fen he 14fen 。ji zhong bai cai gen kang 518zai CRa、CRb、CRc、Crr1、Crr2he Crr3wei dian jun neng kuo zeng chu duo tai xing tiao dai 。6.yi gai cai xing you cai shan bei huang gai （gan bing ）yu CT19（kang bing ）yi ji chan sheng de F2dai wei shi yan cai liao ,gen ju qian ji yu ce de ji yin ,gong she ji 24dui IPyin wu ,zui zhong huo de de 5ge you cha yi IPyin wu 。li yong ji zhong de duo tai xing de IPyin wu IP09-4dui 29fen F2qun ti sao miao ,jie guo fa xian bu fen kang xing biao ji sao miao jie guo bu neng yu kang xing jian ding jie guo xiang dui ying 。zhe shui ming kong zhi gai qun ti kang gen zhong bing de zhu xiao ji yin ke neng bu zai zhe ou yu nei 。7.dui 70fen gai cai xing you cai shan bei huang gai yu CT19de F2qun ti （41fen kang bing 、29fen gan bing ）cai yong jian hua ji yin zu ce xu fang fa dui gen zhong bing kang xing ji yin jin hang ding wei 。gen ju huo de de SNP/InDelbiao ji de xin xi ,ji suan bu tong ji yin xing jian de pin lv cha yi ,yu ce ji yin suo zai ou yu 。jie guo xian shi zai A03ran se ti de 29.3-40.7Mbou jian cun zai yi ge xian zhe QTLwei dian ,gai ou yu jiang hui zuo wei ben yan jiu de hou shua ou yu 。
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论文作者分别是来自西北农林科技大学的梁峰豪，发表于刊物西北农林科技大学2019-07-11论文，是一篇关于根肿病论文,抗病种质筛选论文,抗病种质创制论文,抗病基因定位论文,西北农林科技大学2019-07-11论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自西北农林科技大学2019-07-11论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：根肿病论文; 抗病种质筛选论文; 抗病种质创制论文; 抗病基因定位论文; 西北农林科技大学2019-07-11论文;