本文主要研究内容
作者王小杰（2019）在《耐盐碱乳酸菌对小麦和水稻苗期生长的影响》一文中研究指出：本研究以10株耐盐碱乳酸菌为材料,通过这些乳酸菌对小麦和水稻早期生长特性的促进作用,及对脲酶、蔗糖酶和磷酸酶等土壤酶活性影响的研究,筛选出能促进小麦和水稻生长的优良乳酸菌菌株,并通过筛选菌株在盐碱土壤上促进小麦和水稻早期生长特性的初步研究,探讨乳酸菌对小麦和水稻生长促进菌(PGPB)的可能性。10株乳酸菌均能在pH10和8%盐浓度的条件下生长,表现出极强的耐盐碱性。除了菌株qz201,其它菌株均能在12%高盐浓度下生长,菌株F4-14和菌株F19-30甚至能在16%盐浓度下生长,表现出超强的高盐耐受性。10株受试菌均能产生吲哚乙酸(IAA),表明具有作为PGPB菌株的潜能。菌株N-1、N-19、F4-14、F9-10和F19-30具有解蛋白能力,可将蛋白水解为氨基酸供植物吸收利用。以乳酸菌菌悬液10~9cfu/mL,10~8cfu/mL和10~7cfu/mL三个浓度梯度,浸泡和浇灌两种方案进行盆栽试验。菌株N-1在浇灌浓度为10~9cfu/mL时促进小麦幼苗生长综合效果最好,能显著增加小麦幼苗发芽率、叶绿素、POD活性和还原型谷胱甘肽含量,极显著增加叶鲜重、苗长、SOD和CAT活性,可溶性蛋白含量则无显著变化,且能增加小麦根际土壤酸性磷酸酶和蔗糖酶活性;菌株N-1浸泡浓度为10~7cfu/mL时,除了还原型谷胱甘肽和可溶性蛋白含量无显著差异外,其它值均显著高于对照,且幼苗苗长、SPAD值、SOD和POD活性极显著高于对照。菌株F4-14在浇灌浓度为10~8cfu/mL时促进水稻幼苗生长综合效果最好,能极显著增加水稻幼苗叶鲜重、苗长、叶绿素、SOD、POD和CAT活性,显著增加发芽率和还原型谷胱甘肽含量,且能增加水稻根际土壤脲酶、酸性磷酸酶和蔗糖酶活性;菌株F4-14浸泡浓度为10~9cfu/mL时,除了SPAD值、POD活性和可溶性蛋白含量无显著差异外,其它值均显著高于对照,且幼苗苗长、SOD和CAT活性极显著高于对照。浇灌菌悬液浓度为10~9cfu/mL的菌株N-1后,在盐溶液浓度为0%-2%和pH为7.1-8.5的土壤中,小麦种子发芽率、叶鲜重和苗长大多高于对照,但在pH为8.0时,发芽率略微低于对照;浸泡菌悬液浓度为10~9cfu/mL的菌株N-1后,在盐溶液浓度为0%-2%和pH为7.1、7.3和8.5时的土壤中,小麦种子发芽率、叶鲜重和苗长均高于对照,除在土壤pH为7.5时,苗长也高于对照外,其它值均略低于对照;在盐溶液浓度为0%-2%和pH为7.1-8.5时的土壤中,浇灌菌悬液浓度为10~8cfu/mL的菌株F4-14,水稻种子发芽率均高于对照。本研究表明,菌株N-1和F4-14能促进小麦和水稻幼苗的生长,改善土壤质量,提高盐碱地农作物的发芽率,具有作为生物肥料或PGPB菌株应用于农业领域的潜能。
Abstract
ben yan jiu yi 10zhu nai yan jian ru suan jun wei cai liao ,tong guo zhe xie ru suan jun dui xiao mai he shui dao zao ji sheng chang te xing de cu jin zuo yong ,ji dui niao mei 、zhe tang mei he lin suan mei deng tu rang mei huo xing ying xiang de yan jiu ,shai shua chu neng cu jin xiao mai he shui dao sheng chang de you liang ru suan jun jun zhu ,bing tong guo shai shua jun zhu zai yan jian tu rang shang cu jin xiao mai he shui dao zao ji sheng chang te xing de chu bu yan jiu ,tan tao ru suan jun dui xiao mai he shui dao sheng chang cu jin jun (PGPB)de ke neng xing 。10zhu ru suan jun jun neng zai pH10he 8%yan nong du de tiao jian xia sheng chang ,biao xian chu ji jiang de nai yan jian xing 。chu le jun zhu qz201,ji ta jun zhu jun neng zai 12%gao yan nong du xia sheng chang ,jun zhu F4-14he jun zhu F19-30shen zhi neng zai 16%yan nong du xia sheng chang ,biao xian chu chao jiang de gao yan nai shou xing 。10zhu shou shi jun jun neng chan sheng yin duo yi suan (IAA),biao ming ju you zuo wei PGPBjun zhu de qian neng 。jun zhu N-1、N-19、F4-14、F9-10he F19-30ju you jie dan bai neng li ,ke jiang dan bai shui jie wei an ji suan gong zhi wu xi shou li yong 。yi ru suan jun jun xuan ye 10~9cfu/mL,10~8cfu/mLhe 10~7cfu/mLsan ge nong du ti du ,jin pao he jiao guan liang chong fang an jin hang pen zai shi yan 。jun zhu N-1zai jiao guan nong du wei 10~9cfu/mLshi cu jin xiao mai you miao sheng chang zeng ge xiao guo zui hao ,neng xian zhe zeng jia xiao mai you miao fa ya lv 、xie lu su 、PODhuo xing he hai yuan xing gu guang gan tai han liang ,ji xian zhe zeng jia xie xian chong 、miao chang 、SODhe CAThuo xing ,ke rong xing dan bai han liang ze mo xian zhe bian hua ,ju neng zeng jia xiao mai gen ji tu rang suan xing lin suan mei he zhe tang mei huo xing ;jun zhu N-1jin pao nong du wei 10~7cfu/mLshi ,chu le hai yuan xing gu guang gan tai he ke rong xing dan bai han liang mo xian zhe cha yi wai ,ji ta zhi jun xian zhe gao yu dui zhao ,ju you miao miao chang 、SPADzhi 、SODhe PODhuo xing ji xian zhe gao yu dui zhao 。jun zhu F4-14zai jiao guan nong du wei 10~8cfu/mLshi cu jin shui dao you miao sheng chang zeng ge xiao guo zui hao ,neng ji xian zhe zeng jia shui dao you miao xie xian chong 、miao chang 、xie lu su 、SOD、PODhe CAThuo xing ,xian zhe zeng jia fa ya lv he hai yuan xing gu guang gan tai han liang ,ju neng zeng jia shui dao gen ji tu rang niao mei 、suan xing lin suan mei he zhe tang mei huo xing ;jun zhu F4-14jin pao nong du wei 10~9cfu/mLshi ,chu le SPADzhi 、PODhuo xing he ke rong xing dan bai han liang mo xian zhe cha yi wai ,ji ta zhi jun xian zhe gao yu dui zhao ,ju you miao miao chang 、SODhe CAThuo xing ji xian zhe gao yu dui zhao 。jiao guan jun xuan ye nong du wei 10~9cfu/mLde jun zhu N-1hou ,zai yan rong ye nong du wei 0%-2%he pHwei 7.1-8.5de tu rang zhong ,xiao mai chong zi fa ya lv 、xie xian chong he miao chang da duo gao yu dui zhao ,dan zai pHwei 8.0shi ,fa ya lv lve wei di yu dui zhao ;jin pao jun xuan ye nong du wei 10~9cfu/mLde jun zhu N-1hou ,zai yan rong ye nong du wei 0%-2%he pHwei 7.1、7.3he 8.5shi de tu rang zhong ,xiao mai chong zi fa ya lv 、xie xian chong he miao chang jun gao yu dui zhao ,chu zai tu rang pHwei 7.5shi ,miao chang ye gao yu dui zhao wai ,ji ta zhi jun lve di yu dui zhao ;zai yan rong ye nong du wei 0%-2%he pHwei 7.1-8.5shi de tu rang zhong ,jiao guan jun xuan ye nong du wei 10~8cfu/mLde jun zhu F4-14,shui dao chong zi fa ya lv jun gao yu dui zhao 。ben yan jiu biao ming ,jun zhu N-1he F4-14neng cu jin xiao mai he shui dao you miao de sheng chang ,gai shan tu rang zhi liang ,di gao yan jian de nong zuo wu de fa ya lv ,ju you zuo wei sheng wu fei liao huo PGPBjun zhu ying yong yu nong ye ling yu de qian neng 。
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论文作者分别是来自郑州大学的王小杰，发表于刊物郑州大学2019-07-03论文，是一篇关于乳酸菌论文,小麦论文,水稻论文,酶活性论文,郑州大学2019-07-03论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自郑州大学2019-07-03论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：乳酸菌论文; 小麦论文; 水稻论文; 酶活性论文; 郑州大学2019-07-03论文;