本文主要研究内容
作者吴江萍（2019）在《植物乳杆菌CCFM8610缓解镉暴露诱导肠道损伤的效果评价及机制探究》一文中研究指出：镉(Cadmium,Cd)是一种在环境中广泛存在的重金属,近年来工业化进展的不断加速及污水农田灌溉技术的广泛使用,镉污染事件频频发生,严重危害了公共健康。过往研究表明,植物乳杆菌CCFM8610是一株对重金属镉具有良好的吸附能力的乳酸菌,可有效缓解机体内炎症,预防日常镉暴露。但现阶段,关于植物乳杆菌CCFM8610缓解镉暴露的研究主要集中在肝、肾方面。而肠道作为镉吸收的直接器官,植物乳杆菌CCFM8610对肠道镉暴露损伤的恢复效果及机制却并未有靶向研究。因此,本课题通过体内外实验,对植物乳杆菌CCFM8610恢复镉暴露诱导肠道损伤的效果及其机制进行了探究。首先通过构建慢性镉暴露动物模型,探究植物乳杆菌CCFM8610对慢性镉暴露小鼠肠道损伤的恢复能力。结果表明植物乳杆菌CCFM8610能有效降低小鼠脏器内约20%的镉蓄积,同时加快慢性镉暴露小鼠排便速度,缩短镉暴露小鼠排便时间近2小时,增强小鼠结肠肌条张力。检测小鼠肠道中神经递质,并对小鼠结肠c-kit蛋白进行免疫标记后发现,植物乳杆菌CCFM8610显著调节了镉暴露紊乱的肠道神经递质一氧化氮、5羟色胺、P物质、乙酰胆碱酯酶、血管活性肠肽和降钙素基因相关肽的含量,保护了肠道Cajal细胞,并对肠道运动能力有一定恢复效果。证明植物乳杆菌CCFM8610干预后,能有效预防镉暴露诱导的肠道便秘症状。进一步通过对肠道中JNK、ERK、bcl-2以及膜蛋白的分析发现,植物乳杆菌CCFM8610抑制了肠道ERK和JNK的表达,提高了bcl-2相对表达,促进了肠道细胞的抗凋亡活动,缓解了镉暴露造成的肠道应激与细胞凋亡。同时植物乳杆菌CCFM8610也显著提高了肠道上皮细胞中Claudin、DMT-1和E-cadherin蛋白的相对表达,保护了肠道上皮细胞膜蛋白和肠道屏障。此外,对小鼠肠道菌群的16S测序发现,植物乳杆菌CCFM8610也具有调节肠道菌群,保护肠道微环境的作用。证明植物乳杆菌CCFM8610能通过保护肠道屏障、抑制肠道内炎症和保护肠道菌群稳态这三个途径来保护肠道免受镉暴露造成的损伤。通过构建镉暴露肠上皮(Human colon tumor cell line,HT-29)细胞模型,以蛋白组学手段检测到了4089个可信蛋白,筛选得到168个差异蛋白。基于Gene Ontology数据库、KEGG蛋白注释及蛋白互作分析发现,重金属镉引起了细胞内的压力应激,产生了大量丝裂原活化蛋白激酶(Mitogen-activated protein kinase,MAPK)、核因子-κB(Nuclear factor kappa-light-chain-enhancer of activated B cells,NF-κB)通路相关蛋白,并扰乱了上皮细胞膜上FLAN等蛋白的含量,导致细胞膜结构损坏。使用植物乳杆菌CCFM8610干预细胞后发现,植物乳杆菌CCFM8610有效抑制了镉暴露激活的Ras-Raf-MEK-ERK、NF-κB信号通路,从而缓解了细胞内压力应激和细胞内炎症。此外,植物乳杆菌CCFM8610也降低了镉暴露细胞内蛋白AKR1B10相对表达量,提高了蛋白DDX21和蛋白FLNA的相对表达,从而调节了细胞内能量代谢、RNA和蛋白合成,并通过保护细胞膜蛋白Claudin-1和E-cadherin,维持细胞结构,保护细胞屏障。通过与该实验中其他菌株的对比发现,植物乳杆菌CCFM8610可以通过特意性调节蛋白AKR1B10、DDX21和FLNA的相对表达,从而抑制细胞内炎症保护细胞屏障。与植物乳杆菌CCFM175、CCFM408相比对有一定的菌株特异性。
Abstract
ge (Cadmium,Cd)shi yi chong zai huan jing zhong an fan cun zai de chong jin shu ,jin nian lai gong ye hua jin zhan de bu duan jia su ji wu shui nong tian guan gai ji shu de an fan shi yong ,ge wu ran shi jian pin pin fa sheng ,yan chong wei hai le gong gong jian kang 。guo wang yan jiu biao ming ,zhi wu ru gan jun CCFM8610shi yi zhu dui chong jin shu ge ju you liang hao de xi fu neng li de ru suan jun ,ke you xiao huan jie ji ti nei yan zheng ,yu fang ri chang ge bao lou 。dan xian jie duan ,guan yu zhi wu ru gan jun CCFM8610huan jie ge bao lou de yan jiu zhu yao ji zhong zai gan 、shen fang mian 。er chang dao zuo wei ge xi shou de zhi jie qi guan ,zhi wu ru gan jun CCFM8610dui chang dao ge bao lou sun shang de hui fu xiao guo ji ji zhi que bing wei you ba xiang yan jiu 。yin ci ,ben ke ti tong guo ti nei wai shi yan ,dui zhi wu ru gan jun CCFM8610hui fu ge bao lou you dao chang dao sun shang de xiao guo ji ji ji zhi jin hang le tan jiu 。shou xian tong guo gou jian man xing ge bao lou dong wu mo xing ,tan jiu zhi wu ru gan jun CCFM8610dui man xing ge bao lou xiao shu chang dao sun shang de hui fu neng li 。jie guo biao ming zhi wu ru gan jun CCFM8610neng you xiao jiang di xiao shu zang qi nei yao 20%de ge xu ji ,tong shi jia kuai man xing ge bao lou xiao shu pai bian su du ,su duan ge bao lou xiao shu pai bian shi jian jin 2xiao shi ,zeng jiang xiao shu jie chang ji tiao zhang li 。jian ce xiao shu chang dao zhong shen jing di zhi ,bing dui xiao shu jie chang c-kitdan bai jin hang mian yi biao ji hou fa xian ,zhi wu ru gan jun CCFM8610xian zhe diao jie le ge bao lou wen luan de chang dao shen jing di zhi yi yang hua dan 、5qiang se an 、Pwu zhi 、yi xian dan jian zhi mei 、xie guan huo xing chang tai he jiang gai su ji yin xiang guan tai de han liang ,bao hu le chang dao Cajalxi bao ,bing dui chang dao yun dong neng li you yi ding hui fu xiao guo 。zheng ming zhi wu ru gan jun CCFM8610gan yu hou ,neng you xiao yu fang ge bao lou you dao de chang dao bian bi zheng zhuang 。jin yi bu tong guo dui chang dao zhong JNK、ERK、bcl-2yi ji mo dan bai de fen xi fa xian ,zhi wu ru gan jun CCFM8610yi zhi le chang dao ERKhe JNKde biao da ,di gao le bcl-2xiang dui biao da ,cu jin le chang dao xi bao de kang diao wang huo dong ,huan jie le ge bao lou zao cheng de chang dao ying ji yu xi bao diao wang 。tong shi zhi wu ru gan jun CCFM8610ye xian zhe di gao le chang dao shang pi xi bao zhong Claudin、DMT-1he E-cadherindan bai de xiang dui biao da ,bao hu le chang dao shang pi xi bao mo dan bai he chang dao bing zhang 。ci wai ,dui xiao shu chang dao jun qun de 16Sce xu fa xian ,zhi wu ru gan jun CCFM8610ye ju you diao jie chang dao jun qun ,bao hu chang dao wei huan jing de zuo yong 。zheng ming zhi wu ru gan jun CCFM8610neng tong guo bao hu chang dao bing zhang 、yi zhi chang dao nei yan zheng he bao hu chang dao jun qun wen tai zhe san ge tu jing lai bao hu chang dao mian shou ge bao lou zao cheng de sun shang 。tong guo gou jian ge bao lou chang shang pi (Human colon tumor cell line,HT-29)xi bao mo xing ,yi dan bai zu xue shou duan jian ce dao le 4089ge ke xin dan bai ,shai shua de dao 168ge cha yi dan bai 。ji yu Gene Ontologyshu ju ku 、KEGGdan bai zhu shi ji dan bai hu zuo fen xi fa xian ,chong jin shu ge yin qi le xi bao nei de ya li ying ji ,chan sheng le da liang si lie yuan huo hua dan bai ji mei (Mitogen-activated protein kinase,MAPK)、he yin zi -κB(Nuclear factor kappa-light-chain-enhancer of activated B cells,NF-κB)tong lu xiang guan dan bai ,bing rao luan le shang pi xi bao mo shang FLANdeng dan bai de han liang ,dao zhi xi bao mo jie gou sun huai 。shi yong zhi wu ru gan jun CCFM8610gan yu xi bao hou fa xian ,zhi wu ru gan jun CCFM8610you xiao yi zhi le ge bao lou ji huo de Ras-Raf-MEK-ERK、NF-κBxin hao tong lu ,cong er huan jie le xi bao nei ya li ying ji he xi bao nei yan zheng 。ci wai ,zhi wu ru gan jun CCFM8610ye jiang di le ge bao lou xi bao nei dan bai AKR1B10xiang dui biao da liang ,di gao le dan bai DDX21he dan bai FLNAde xiang dui biao da ,cong er diao jie le xi bao nei neng liang dai xie 、RNAhe dan bai ge cheng ,bing tong guo bao hu xi bao mo dan bai Claudin-1he E-cadherin,wei chi xi bao jie gou ,bao hu xi bao bing zhang 。tong guo yu gai shi yan zhong ji ta jun zhu de dui bi fa xian ,zhi wu ru gan jun CCFM8610ke yi tong guo te yi xing diao jie dan bai AKR1B10、DDX21he FLNAde xiang dui biao da ,cong er yi zhi xi bao nei yan zheng bao hu xi bao bing zhang 。yu zhi wu ru gan jun CCFM175、CCFM408xiang bi dui you yi ding de jun zhu te yi xing 。
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论文详细介绍
论文作者分别是来自江南大学的吴江萍，发表于刊物江南大学2019-10-21论文，是一篇关于植物乳杆菌论文,镉暴露论文,丝裂原活化蛋白激酶论文,肠道论文,江南大学2019-10-21论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自江南大学2019-10-21论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：植物乳杆菌论文; 镉暴露论文; 丝裂原活化蛋白激酶论文; 肠道论文; 江南大学2019-10-21论文;