本文主要研究内容
作者倪湾（2019）在《洋葱槲皮素抗炎活性及对NF-κB和Akt信号通路的影响》一文中研究指出：洋葱属百合科葱属二年生草本植物,因富含槲皮素、前列腺素A等重要生物活性物质,而成为具有多种营养价值的蔬菜。洋葱槲皮素(onion quercetin,OQE)是洋葱中提取的一种多羟基黄酮类化合物,现代研究表明,洋葱槲皮素具有抗炎、抗氧化、抗癌、降糖、降压、免疫调节及保护心血管等功效。为探讨洋葱槲皮素的抗炎作用,本文采用1μg/mL脂多糖处理小鼠腹腔巨噬细胞建立细胞炎症模型,体外研究洋葱槲皮素的抗炎活性及其作用机制,为开发抗炎功能性食品提供实验依据。本文的研究方法和结果如下:1.体外建立LPS诱导的体外小鼠腹腔巨噬细胞炎症模型,采用MTT法检测OQE单药组、OQE与LPS复合药组对小鼠腹腔巨噬细胞吞噬功能的影响;结果表明1μg/mL LPS作用小鼠腹腔巨噬细胞24 h,在20~160μg/mL剂量范围内,OQE单药组刺激、OQE与LPS复合药组均能提高小鼠腹腔巨噬细胞的吞噬能力。2.采用ELISA法检测OQE对LPS诱导的小鼠腹腔巨噬细胞分泌细胞因子及炎症介质IL-1β、COX-2、IL-6、和TNF-α的影响;结果表明1μg/mL LPS作用小鼠腹腔巨噬细胞24 h,OQE处理组均可抑制LPS诱导的小鼠腹腔巨噬细胞IL-1β和COX-2、IL-6和TNF-α的分泌,且随着OQE浓度增加,抑制作用增强,存在着明显的剂量-效应关系。3.采用RT-PCR法检测OQE对LPS诱导的小鼠腹腔巨噬细胞分泌细胞因子及炎症介质基因IL-1β、COX-2、IL-6和TNF-α的mRNA表达水平;结果表明1μg/mL LPS作用小鼠腹腔巨噬细胞24 h,OQE处理组均可抑制LPS诱导的小鼠腹腔巨噬细胞分泌细胞因子及炎症介质基因IL-1β、COX-2、IL-6和TNF-α的mRNA表达水平,且随着OQE浓度增加,抑制作用增强,存在着明显的剂量-效应关系;OQE可抑制LPS诱导的小鼠腹腔巨噬细胞炎症反应,具有抗炎作用。4.采用Western Blot法检测OQE对LPS诱导的小鼠腹腔巨噬细胞中NF-κB和Akt信号通路蛋白磷酸化水平表达的影响;结果表明1μg/mL LPS作用小鼠腹腔巨噬细胞24h,LPS刺激可以显著上调小鼠腹腔巨噬细胞中NF-κB-p65和Akt蛋白磷酸化水平,而OQE处理组均可抑制LPS诱导的NF-κB-p65和Akt蛋白磷酸化水平;通过对NF-κB和Akt信号通路的研究,发现OQE可抑制NF-κB和Akt信号转导通路进而抑制炎症因子的表达,从而发挥抗炎作用。
Abstract
xiang cong shu bai ge ke cong shu er nian sheng cao ben zhi wu ,yin fu han hu pi su 、qian lie xian su Adeng chong yao sheng wu huo xing wu zhi ,er cheng wei ju you duo chong ying yang jia zhi de shu cai 。xiang cong hu pi su (onion quercetin,OQE)shi xiang cong zhong di qu de yi chong duo qiang ji huang tong lei hua ge wu ,xian dai yan jiu biao ming ,xiang cong hu pi su ju you kang yan 、kang yang hua 、kang ai 、jiang tang 、jiang ya 、mian yi diao jie ji bao hu xin xie guan deng gong xiao 。wei tan tao xiang cong hu pi su de kang yan zuo yong ,ben wen cai yong 1μg/mLzhi duo tang chu li xiao shu fu qiang ju shi xi bao jian li xi bao yan zheng mo xing ,ti wai yan jiu xiang cong hu pi su de kang yan huo xing ji ji zuo yong ji zhi ,wei kai fa kang yan gong neng xing shi pin di gong shi yan yi ju 。ben wen de yan jiu fang fa he jie guo ru xia :1.ti wai jian li LPSyou dao de ti wai xiao shu fu qiang ju shi xi bao yan zheng mo xing ,cai yong MTTfa jian ce OQEchan yao zu 、OQEyu LPSfu ge yao zu dui xiao shu fu qiang ju shi xi bao tun shi gong neng de ying xiang ;jie guo biao ming 1μg/mL LPSzuo yong xiao shu fu qiang ju shi xi bao 24 h,zai 20~160μg/mLji liang fan wei nei ,OQEchan yao zu ci ji 、OQEyu LPSfu ge yao zu jun neng di gao xiao shu fu qiang ju shi xi bao de tun shi neng li 。2.cai yong ELISAfa jian ce OQEdui LPSyou dao de xiao shu fu qiang ju shi xi bao fen bi xi bao yin zi ji yan zheng jie zhi IL-1β、COX-2、IL-6、he TNF-αde ying xiang ;jie guo biao ming 1μg/mL LPSzuo yong xiao shu fu qiang ju shi xi bao 24 h,OQEchu li zu jun ke yi zhi LPSyou dao de xiao shu fu qiang ju shi xi bao IL-1βhe COX-2、IL-6he TNF-αde fen bi ,ju sui zhao OQEnong du zeng jia ,yi zhi zuo yong zeng jiang ,cun zai zhao ming xian de ji liang -xiao ying guan ji 。3.cai yong RT-PCRfa jian ce OQEdui LPSyou dao de xiao shu fu qiang ju shi xi bao fen bi xi bao yin zi ji yan zheng jie zhi ji yin IL-1β、COX-2、IL-6he TNF-αde mRNAbiao da shui ping ;jie guo biao ming 1μg/mL LPSzuo yong xiao shu fu qiang ju shi xi bao 24 h,OQEchu li zu jun ke yi zhi LPSyou dao de xiao shu fu qiang ju shi xi bao fen bi xi bao yin zi ji yan zheng jie zhi ji yin IL-1β、COX-2、IL-6he TNF-αde mRNAbiao da shui ping ,ju sui zhao OQEnong du zeng jia ,yi zhi zuo yong zeng jiang ,cun zai zhao ming xian de ji liang -xiao ying guan ji ;OQEke yi zhi LPSyou dao de xiao shu fu qiang ju shi xi bao yan zheng fan ying ,ju you kang yan zuo yong 。4.cai yong Western Blotfa jian ce OQEdui LPSyou dao de xiao shu fu qiang ju shi xi bao zhong NF-κBhe Aktxin hao tong lu dan bai lin suan hua shui ping biao da de ying xiang ;jie guo biao ming 1μg/mL LPSzuo yong xiao shu fu qiang ju shi xi bao 24h,LPSci ji ke yi xian zhe shang diao xiao shu fu qiang ju shi xi bao zhong NF-κB-p65he Aktdan bai lin suan hua shui ping ,er OQEchu li zu jun ke yi zhi LPSyou dao de NF-κB-p65he Aktdan bai lin suan hua shui ping ;tong guo dui NF-κBhe Aktxin hao tong lu de yan jiu ,fa xian OQEke yi zhi NF-κBhe Aktxin hao zhuai dao tong lu jin er yi zhi yan zheng yin zi de biao da ,cong er fa hui kang yan zuo yong 。
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论文作者分别是来自锦州医科大学的倪湾，发表于刊物锦州医科大学2019-08-06论文，是一篇关于洋葱槲皮素论文,脂多糖论文,巨噬细胞论文,核转录因子论文,蛋白激酶论文,锦州医科大学2019-08-06论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自锦州医科大学2019-08-06论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：洋葱槲皮素论文; 脂多糖论文; 巨噬细胞论文; 核转录因子论文; 蛋白激酶论文; 锦州医科大学2019-08-06论文;