本文主要研究内容
作者杨景（2019）在《黏虫对Bt毒素的抗性演化》一文中研究指出：黏虫Mythimna separata（Walker）是一种广泛分布于亚洲和澳州的重大农业害虫,玉米是黏虫严重危害的作物之一。全生育期高效表达特定苏云金杆菌Bacillus thuringiensis即Bt毒素的转基因抗虫玉米（Bt玉米）为黏虫的防治提供了新途径。全球20多个国家种植Bt玉米实践表明,靶标害虫抗性的产生将严重威胁Bt玉米的可持续应用。实施有效的抗性治理策略如高剂量庇护所、多基因策略可延缓甚或避免抗性的产生。明确害虫对Bt毒素的抗性演化及遗传规律是制定抗性治理策略的理论基础和科学依据。本文应用毒力生测法评价了Cry1Ab、Cry1Ac、Cry1Ah、Cry1F、Cry2Aa、Cry2Ab、Cry1Ie、Vip3Aa19、Vip3Aa16、Vip3Ca 10种Bt毒素对黏虫的毒力及Cry类蛋白与Vip3类蛋白间的增效作用;以室内汰选法研究了黏虫对Cry1Ab、Cry1F、Vip3Aa19蛋白的抗性发展规律,进而研究了Vip3Aa19抗性品系的适合度代价与抗性遗传规律。黏虫毒力生测结果表明:10种Bt毒素对黏虫的致死中浓度LC50值从1.6μg/g到78.6μg/g。与Cry1A类和Cry2Aa蛋白相比,Vip3类蛋白有较强的杀虫活性,LC50值从1.6到7.4μg/g,其中,Vip3Aa19毒力最强,LC50值仅为1.6μg/g。Cry类蛋白与Vip3A类蛋白之间存在增效作用,增效因子为2.29.2,Vip3Aa16与Cry1毒素的相互作用程度较高,而Vip3Ca蛋白与其他蛋白间均不存在增效作用。用Cry1Ab、Cry1F、Vip3Aa19 3种蛋白开展了黏虫抗性汰选,在Cry1Ab 75%80%和99%的汰选压、Cry1F 65%72%的汰选压以及Vip3Aa19 95%99%的汰选压饲养初孵黏虫,710 d后将存活幼虫转至普通饲料饲养。Vip3Aa19蛋白连续汰选的黏虫种群,其抗性发展迅速,汰选2代后抗性倍数达278.10倍;8代后,该种群对Vip3Aa19蛋白的抗性水平达到3000多倍。Cry1F蛋白连续汰选10代后,该种群对Cry1F蛋白的敏感性同对照种群没有显著差异。分别处于Cry1Ab高、低汰选压下的黏虫种群,在连续汰选5代和8代后,抗性倍数均未发生显著变化。Vip3Aa19抗性品系对Cry1Ab和Cry1F的交互抗性水平分别为2.79倍和0.58倍,即对Cry1Ab产生较低水平的交互抗性,对Cry1F未产生交互抗性。Vip3Aa19抗性品系的幼虫期比敏感品系显著延长2.8 d,羽化率降低34%。抗性品系比敏感品系的平均单雌产卵量下降29.5%,而当抗性品系幼虫期取食非汰选饲料（不含Bt蛋白的人工饲料）时,其平均单雌产卵量显著下降39.1%,即有显著的适应性代价。Vip3Aa19抗性品系抗性显性水平与浓度相关,在高剂量下表现部分隐性遗传,低剂量下表现为部分显性遗传。以抗、感品系正反交后代的LC50计算的抗性基因显性水平（h）分别为0.68和0.33,这表明对Vip3Aa19杀虫蛋白的抗性遗传为受母性影响的不完全显性遗传。
Abstract
nian chong Mythimna separata（Walker）shi yi chong an fan fen bu yu ya zhou he ao zhou de chong da nong ye hai chong ,yu mi shi nian chong yan chong wei hai de zuo wu zhi yi 。quan sheng yo ji gao xiao biao da te ding su yun jin gan jun Bacillus thuringiensisji Btdu su de zhuai ji yin kang chong yu mi （Btyu mi ）wei nian chong de fang zhi di gong le xin tu jing 。quan qiu 20duo ge guo jia chong zhi Btyu mi shi jian biao ming ,ba biao hai chong kang xing de chan sheng jiang yan chong wei xie Btyu mi de ke chi xu ying yong 。shi shi you xiao de kang xing zhi li ce lve ru gao ji liang bi hu suo 、duo ji yin ce lve ke yan huan shen huo bi mian kang xing de chan sheng 。ming que hai chong dui Btdu su de kang xing yan hua ji wei chuan gui lv shi zhi ding kang xing zhi li ce lve de li lun ji chu he ke xue yi ju 。ben wen ying yong du li sheng ce fa ping jia le Cry1Ab、Cry1Ac、Cry1Ah、Cry1F、Cry2Aa、Cry2Ab、Cry1Ie、Vip3Aa19、Vip3Aa16、Vip3Ca 10chong Btdu su dui nian chong de du li ji Crylei dan bai yu Vip3lei dan bai jian de zeng xiao zuo yong ;yi shi nei tai shua fa yan jiu le nian chong dui Cry1Ab、Cry1F、Vip3Aa19dan bai de kang xing fa zhan gui lv ,jin er yan jiu le Vip3Aa19kang xing pin ji de kuo ge du dai jia yu kang xing wei chuan gui lv 。nian chong du li sheng ce jie guo biao ming :10chong Btdu su dui nian chong de zhi si zhong nong du LC50zhi cong 1.6μg/gdao 78.6μg/g。yu Cry1Alei he Cry2Aadan bai xiang bi ,Vip3lei dan bai you jiao jiang de sha chong huo xing ,LC50zhi cong 1.6dao 7.4μg/g,ji zhong ,Vip3Aa19du li zui jiang ,LC50zhi jin wei 1.6μg/g。Crylei dan bai yu Vip3Alei dan bai zhi jian cun zai zeng xiao zuo yong ,zeng xiao yin zi wei 2.29.2,Vip3Aa16yu Cry1du su de xiang hu zuo yong cheng du jiao gao ,er Vip3Cadan bai yu ji ta dan bai jian jun bu cun zai zeng xiao zuo yong 。yong Cry1Ab、Cry1F、Vip3Aa19 3chong dan bai kai zhan le nian chong kang xing tai shua ,zai Cry1Ab 75%80%he 99%de tai shua ya 、Cry1F 65%72%de tai shua ya yi ji Vip3Aa19 95%99%de tai shua ya si yang chu fu nian chong ,710 dhou jiang cun huo you chong zhuai zhi pu tong si liao si yang 。Vip3Aa19dan bai lian xu tai shua de nian chong chong qun ,ji kang xing fa zhan xun su ,tai shua 2dai hou kang xing bei shu da 278.10bei ;8dai hou ,gai chong qun dui Vip3Aa19dan bai de kang xing shui ping da dao 3000duo bei 。Cry1Fdan bai lian xu tai shua 10dai hou ,gai chong qun dui Cry1Fdan bai de min gan xing tong dui zhao chong qun mei you xian zhe cha yi 。fen bie chu yu Cry1Abgao 、di tai shua ya xia de nian chong chong qun ,zai lian xu tai shua 5dai he 8dai hou ,kang xing bei shu jun wei fa sheng xian zhe bian hua 。Vip3Aa19kang xing pin ji dui Cry1Abhe Cry1Fde jiao hu kang xing shui ping fen bie wei 2.79bei he 0.58bei ,ji dui Cry1Abchan sheng jiao di shui ping de jiao hu kang xing ,dui Cry1Fwei chan sheng jiao hu kang xing 。Vip3Aa19kang xing pin ji de you chong ji bi min gan pin ji xian zhe yan chang 2.8 d,yu hua lv jiang di 34%。kang xing pin ji bi min gan pin ji de ping jun chan ci chan luan liang xia jiang 29.5%,er dang kang xing pin ji you chong ji qu shi fei tai shua si liao （bu han Btdan bai de ren gong si liao ）shi ,ji ping jun chan ci chan luan liang xian zhe xia jiang 39.1%,ji you xian zhe de kuo ying xing dai jia 。Vip3Aa19kang xing pin ji kang xing xian xing shui ping yu nong du xiang guan ,zai gao ji liang xia biao xian bu fen yin xing wei chuan ,di ji liang xia biao xian wei bu fen xian xing wei chuan 。yi kang 、gan pin ji zheng fan jiao hou dai de LC50ji suan de kang xing ji yin xian xing shui ping （h）fen bie wei 0.68he 0.33,zhe biao ming dui Vip3Aa19sha chong dan bai de kang xing wei chuan wei shou mu xing ying xiang de bu wan quan xian xing wei chuan 。
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论文详细介绍
论文作者分别是来自中国农业科学院的杨景，发表于刊物中国农业科学院2019-07-05论文，是一篇关于黏虫论文,抗性演化论文,抗性遗传规律论文,中国农业科学院2019-07-05论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自中国农业科学院2019-07-05论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：黏虫论文; 抗性演化论文; 抗性遗传规律论文; 中国农业科学院2019-07-05论文;