本文主要研究内容
作者唐莹（2019）在《多肽-纳米簇荧光传感器的设计合成及应用研究》一文中研究指出：金纳米簇(AuNCs)作为新兴的荧光纳米材料,是由几个至一百个不等的金原子组建而成,其直径一般不到2 nm,同时具有孤立原子与纳米粒子的性质。贵金属纳米簇具有较强的光致发光、Stokes位移大、稳定性强等光学性质,已被广泛应用于环境、生物分析和医学诊断等多个领域。本文利用多肽-金属纳米簇和硫化镉(CdS)量子点之间的荧光共振能量转移(FRET),对食品添加剂偶氮类色素诱惑红、胰岛素以及凝血酶等生物大分子进行检测,主要研究内容如下:1.设计功能性多肽(CCYRGHDHCY)用来修饰金纳米簇(AuNCs),制备多肽-金纳米簇(peptide-AuNCs)荧光传感器,并对诱惑红进行定量检测。当诱惑红存在时,多肽中的氨基酸与诱惑红之间由于静电引力作用而相互吸附,发生电子转移,导致peptide-AuNCs大量团聚,造成体系的荧光猝灭。在浓度范围5-90μM内诱惑红浓度与荧光比率成良好的线性相关,线性方程为y=0.0164x+0.99073(R~2=0.998),检出限为0.667μmol/L。并应用该荧光传感器检测饮料样品中的诱惑红。2.以CdS量子点和功能性多肽-金纳米簇(CCYRRKRRHCY-AuNCs)作为供体和受体,基于二者之间的FRET设计了“turn on”荧光传感体系,并对胰岛素进行识别检测。通过体系荧光的猝灭和恢复,可以灵敏地检测胰岛素浓度,在20-500 ng/mL浓度范围内,胰岛素浓度与荧光恢复比率呈良好的线性关系,线性方程为y=12.359x+0.2591(R~2=0.996),检出限低至6.67 ng/mL,并应用该荧光传感体系对人体血清样品中的胰岛素进行筛查和定量检测。3.基于功能性多肽-银纳米簇(CCYRGWRGCY-AgNCs)和CdS量子点之间的FRET构建“turn on”荧光传感体系,并用于凝血酶的分析检测。凝血酶能够裂解精氨酸(R)和甘氨酸(G)间的肽键,从而与peptide-AgNCs特异性识别。凝血酶能够削弱peptide-AgNCs和CdS量子点间的FRET,通过体系荧光的猝灭和恢复,对凝血酶进行定量检测。在5-60 ng/mL浓度范围内凝血酶与荧光恢复比率呈良好的线性关系,线性方程为y=0.0591x+0.0691(R~2=0.997),检出限为1.67 ng/mL。该荧光传感体系可以满足现场快速筛查血清样品中凝血酶的需求。
Abstract
jin na mi cu (AuNCs)zuo wei xin xing de ying guang na mi cai liao ,shi you ji ge zhi yi bai ge bu deng de jin yuan zi zu jian er cheng ,ji zhi jing yi ban bu dao 2 nm,tong shi ju you gu li yuan zi yu na mi li zi de xing zhi 。gui jin shu na mi cu ju you jiao jiang de guang zhi fa guang 、Stokeswei yi da 、wen ding xing jiang deng guang xue xing zhi ,yi bei an fan ying yong yu huan jing 、sheng wu fen xi he yi xue zhen duan deng duo ge ling yu 。ben wen li yong duo tai -jin shu na mi cu he liu hua ge (CdS)liang zi dian zhi jian de ying guang gong zhen neng liang zhuai yi (FRET),dui shi pin tian jia ji ou dan lei se su you huo gong 、yi dao su yi ji ning xie mei deng sheng wu da fen zi jin hang jian ce ,zhu yao yan jiu nei rong ru xia :1.she ji gong neng xing duo tai (CCYRGHDHCY)yong lai xiu shi jin na mi cu (AuNCs),zhi bei duo tai -jin na mi cu (peptide-AuNCs)ying guang chuan gan qi ,bing dui you huo gong jin hang ding liang jian ce 。dang you huo gong cun zai shi ,duo tai zhong de an ji suan yu you huo gong zhi jian you yu jing dian yin li zuo yong er xiang hu xi fu ,fa sheng dian zi zhuai yi ,dao zhi peptide-AuNCsda liang tuan ju ,zao cheng ti ji de ying guang cu mie 。zai nong du fan wei 5-90μMnei you huo gong nong du yu ying guang bi lv cheng liang hao de xian xing xiang guan ,xian xing fang cheng wei y=0.0164x+0.99073(R~2=0.998),jian chu xian wei 0.667μmol/L。bing ying yong gai ying guang chuan gan qi jian ce yin liao yang pin zhong de you huo gong 。2.yi CdSliang zi dian he gong neng xing duo tai -jin na mi cu (CCYRRKRRHCY-AuNCs)zuo wei gong ti he shou ti ,ji yu er zhe zhi jian de FRETshe ji le “turn on”ying guang chuan gan ti ji ,bing dui yi dao su jin hang shi bie jian ce 。tong guo ti ji ying guang de cu mie he hui fu ,ke yi ling min de jian ce yi dao su nong du ,zai 20-500 ng/mLnong du fan wei nei ,yi dao su nong du yu ying guang hui fu bi lv cheng liang hao de xian xing guan ji ,xian xing fang cheng wei y=12.359x+0.2591(R~2=0.996),jian chu xian di zhi 6.67 ng/mL,bing ying yong gai ying guang chuan gan ti ji dui ren ti xie qing yang pin zhong de yi dao su jin hang shai cha he ding liang jian ce 。3.ji yu gong neng xing duo tai -yin na mi cu (CCYRGWRGCY-AgNCs)he CdSliang zi dian zhi jian de FRETgou jian “turn on”ying guang chuan gan ti ji ,bing yong yu ning xie mei de fen xi jian ce 。ning xie mei neng gou lie jie jing an suan (R)he gan an suan (G)jian de tai jian ,cong er yu peptide-AgNCste yi xing shi bie 。ning xie mei neng gou xiao ruo peptide-AgNCshe CdSliang zi dian jian de FRET,tong guo ti ji ying guang de cu mie he hui fu ,dui ning xie mei jin hang ding liang jian ce 。zai 5-60 ng/mLnong du fan wei nei ning xie mei yu ying guang hui fu bi lv cheng liang hao de xian xing guan ji ,xian xing fang cheng wei y=0.0591x+0.0691(R~2=0.997),jian chu xian wei 1.67 ng/mL。gai ying guang chuan gan ti ji ke yi man zu xian chang kuai su shai cha xie qing yang pin zhong ning xie mei de xu qiu 。
论文参考文献
论文详细介绍
论文作者分别是来自吉林大学的唐莹，发表于刊物吉林大学2019-06-25论文，是一篇关于金属纳米簇论文,多肽论文,量子点论文,荧光共振能量转移论文,吉林大学2019-06-25论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自吉林大学2019-06-25论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：金属纳米簇论文; 多肽论文; 量子点论文; 荧光共振能量转移论文; 吉林大学2019-06-25论文;