本文主要研究内容
作者杨兴卓（2019）在《两种草原毛虫肠道菌多样性的比较分析》一文中研究指出：草原毛虫是我国青藏高原高寒草甸的重大害虫,不仅取食优质牧草与畜争食,而且可改变高寒草甸的植被群落结构,已成为高寒草甸持续利用及健康发展的重要限制因素之一。草原毛虫大面积暴发、持续危害,显示出其对高寒草甸低氧、低温及强紫外辐射等极端生态环境的良好适应性。本论文基于分离培养法及高通量测序技术,比较分析了栖息于高寒草甸不同海拔的门源草原毛虫和黄斑草原毛虫的肠道菌组成、多样性及功能,旨在从肠道菌的视角解析草原毛虫对高海拔环境适应的生态学机制。主要结果如下:1.基于6种培养基,对两种草原毛虫5龄幼虫的肠道菌进行分离、培养及纯化,并通过菌落形态特征及16S测序进行菌种鉴定。结果显示,LB培养基、牛肉膏蛋白胨培养基、高氏培养基、无机盐培养基,沙门菌培养基,这5种培养基能够培养草原毛虫肠道菌。门源草原毛虫共分离获得5种肠道菌,其中3种(M1、M2、M3)属厚壁菌门的葡萄球菌属,另2种属变形菌门的Lelliottia(M4)和勒克氏菌属(M5)。黄斑草原毛虫共分离得到6种肠道菌,其中3种属厚壁菌门的葡萄球菌(N1和N5)和肠球菌属(N4),其余3种属变形菌门的沙雷氏菌属(N2和N6)和假单胞菌属(N3)。沙雷氏菌已知在协助昆虫降解纤维素、木质素上具有积极作用,提示该菌也有可能协助黄斑草原毛虫降解植物纤维。2.门源草原毛虫肠道菌16S扩增子共获得了57个OTU,注释到:4个门,其中优势门为变形菌门;7个纲,优势纲为α变形菌纲和γ变形菌纲;12个目,优势目为立克次氏体目和假单胞菌目;15个科,优势科为立克次氏体科(83.8%)和假单胞杆菌科;13个属,优势属为Wolbachia和假单胞菌属;7个种,优势种为酪黄肠球菌、Staphylococcus sciuri和Methylotenera mobilis。门源草原毛虫肠道宏基因组共注释到:5个界,相对丰度最高的是细菌界(71.8%)和真菌界(12.1%);27个门,优势门为变形菌门、厚壁菌门和拟杆菌门;51个纲,优势纲为α变形菌纲、γ变形菌纲和芽孢杆菌纲;107个目,优势目为立克次氏体目、肠球菌目和芽孢杆菌目;178个科,优势科为无形体科、肠杆菌科;236个属,优势属为Wolbachia、埃希氏菌属。3.黄斑草原毛虫肠道菌16S扩增子测序共获得了91个OTU,注释到:5个门,厚壁菌门相对丰度最高;7个纲,优势纲为杆菌纲和γ变形菌纲;10个目,优势目为乳杆菌目和芽孢杆菌目;11个科,优势科为肉杆菌科和肠球菌科;9个属,优势属为肉食杆菌属和肠球菌属;8个种,优势种为铅黄肠球菌和壁芽孢杆菌。黄斑草原毛虫肠道宏基因组共注释到:5个界,相对丰度最高的为细菌界(57.3%)、真菌界(17.7%)和病毒界(11.6%);27个门,优势门为变形菌门、拟杆菌门和厚壁菌门;56个纲,优势纲为γ变形菌纲、芽孢杆菌纲和纤维黏网菌;119个目,优势目为肠杆菌目、芽孢杆菌目和噬纤维菌目;200个科,优势科为肠杆菌科、动球菌科和Cyclobacteriaceae;262个属,优势属为埃希氏菌属、土壤芽孢杆菌属和Cecembia。4.草原毛虫肠道宏基因组注释到与新陈代谢、碳水化合物运输、氨基酸合成等代谢密切相关的基因,表明肠道菌可能参与了草原毛虫的碳水化物代谢、物质合成、物质降解及吸收等生理过程,在草原毛虫适应高寒草甸极端生态环境中发挥重要作用。16S扩增子测序表明,门源草原毛虫肠道菌相对丰度较高的为Wolbachia和假单胞菌,而黄斑草原毛虫肠道菌相对丰度较高的为肉食杆菌属和肠球菌。这种差异可能与两种草原毛虫栖息的差异性海拔环境相关。门源草原毛虫肠道菌α多样性未显著高于黄斑草原毛虫(P>0.05),但在不考虑肠道菌相对丰度的情况下,不动杆菌属、无色杆菌、芽单胞菌属、噬氢菌属、甲氧菌属、Wolbachia、肠球菌属、假单胞菌属和肉食杆菌属等9个属在两种草原毛虫间差异显著(P<0.01)。据此推测,这些菌可能与两种草原毛虫适应差异性海拔环境相关。
Abstract
cao yuan mao chong shi wo guo qing cang gao yuan gao han cao dian de chong da hai chong ,bu jin qu shi you zhi mu cao yu chu zheng shi ,er ju ke gai bian gao han cao dian de zhi bei qun la jie gou ,yi cheng wei gao han cao dian chi xu li yong ji jian kang fa zhan de chong yao xian zhi yin su zhi yi 。cao yuan mao chong da mian ji bao fa 、chi xu wei hai ,xian shi chu ji dui gao han cao dian di yang 、di wen ji jiang zi wai fu she deng ji duan sheng tai huan jing de liang hao kuo ying xing 。ben lun wen ji yu fen li pei yang fa ji gao tong liang ce xu ji shu ,bi jiao fen xi le qi xi yu gao han cao dian bu tong hai ba de men yuan cao yuan mao chong he huang ban cao yuan mao chong de chang dao jun zu cheng 、duo yang xing ji gong neng ,zhi zai cong chang dao jun de shi jiao jie xi cao yuan mao chong dui gao hai ba huan jing kuo ying de sheng tai xue ji zhi 。zhu yao jie guo ru xia :1.ji yu 6chong pei yang ji ,dui liang chong cao yuan mao chong 5ling you chong de chang dao jun jin hang fen li 、pei yang ji chun hua ,bing tong guo jun la xing tai te zheng ji 16Sce xu jin hang jun chong jian ding 。jie guo xian shi ,LBpei yang ji 、niu rou gao dan bai dong pei yang ji 、gao shi pei yang ji 、mo ji yan pei yang ji ,sha men jun pei yang ji ,zhe 5chong pei yang ji neng gou pei yang cao yuan mao chong chang dao jun 。men yuan cao yuan mao chong gong fen li huo de 5chong chang dao jun ,ji zhong 3chong (M1、M2、M3)shu hou bi jun men de pu tao qiu jun shu ,ling 2chong shu bian xing jun men de Lelliottia(M4)he le ke shi jun shu (M5)。huang ban cao yuan mao chong gong fen li de dao 6chong chang dao jun ,ji zhong 3chong shu hou bi jun men de pu tao qiu jun (N1he N5)he chang qiu jun shu (N4),ji yu 3chong shu bian xing jun men de sha lei shi jun shu (N2he N6)he jia chan bao jun shu (N3)。sha lei shi jun yi zhi zai xie zhu kun chong jiang jie qian wei su 、mu zhi su shang ju you ji ji zuo yong ,di shi gai jun ye you ke neng xie zhu huang ban cao yuan mao chong jiang jie zhi wu qian wei 。2.men yuan cao yuan mao chong chang dao jun 16Skuo zeng zi gong huo de le 57ge OTU,zhu shi dao :4ge men ,ji zhong you shi men wei bian xing jun men ;7ge gang ,you shi gang wei αbian xing jun gang he γbian xing jun gang ;12ge mu ,you shi mu wei li ke ci shi ti mu he jia chan bao jun mu ;15ge ke ,you shi ke wei li ke ci shi ti ke (83.8%)he jia chan bao gan jun ke ;13ge shu ,you shi shu wei Wolbachiahe jia chan bao jun shu ;7ge chong ,you shi chong wei lao huang chang qiu jun 、Staphylococcus sciurihe Methylotenera mobilis。men yuan cao yuan mao chong chang dao hong ji yin zu gong zhu shi dao :5ge jie ,xiang dui feng du zui gao de shi xi jun jie (71.8%)he zhen jun jie (12.1%);27ge men ,you shi men wei bian xing jun men 、hou bi jun men he ni gan jun men ;51ge gang ,you shi gang wei αbian xing jun gang 、γbian xing jun gang he ya bao gan jun gang ;107ge mu ,you shi mu wei li ke ci shi ti mu 、chang qiu jun mu he ya bao gan jun mu ;178ge ke ,you shi ke wei mo xing ti ke 、chang gan jun ke ;236ge shu ,you shi shu wei Wolbachia、ai xi shi jun shu 。3.huang ban cao yuan mao chong chang dao jun 16Skuo zeng zi ce xu gong huo de le 91ge OTU,zhu shi dao :5ge men ,hou bi jun men xiang dui feng du zui gao ;7ge gang ,you shi gang wei gan jun gang he γbian xing jun gang ;10ge mu ,you shi mu wei ru gan jun mu he ya bao gan jun mu ;11ge ke ,you shi ke wei rou gan jun ke he chang qiu jun ke ;9ge shu ,you shi shu wei rou shi gan jun shu he chang qiu jun shu ;8ge chong ,you shi chong wei qian huang chang qiu jun he bi ya bao gan jun 。huang ban cao yuan mao chong chang dao hong ji yin zu gong zhu shi dao :5ge jie ,xiang dui feng du zui gao de wei xi jun jie (57.3%)、zhen jun jie (17.7%)he bing du jie (11.6%);27ge men ,you shi men wei bian xing jun men 、ni gan jun men he hou bi jun men ;56ge gang ,you shi gang wei γbian xing jun gang 、ya bao gan jun gang he qian wei nian wang jun ;119ge mu ,you shi mu wei chang gan jun mu 、ya bao gan jun mu he shi qian wei jun mu ;200ge ke ,you shi ke wei chang gan jun ke 、dong qiu jun ke he Cyclobacteriaceae;262ge shu ,you shi shu wei ai xi shi jun shu 、tu rang ya bao gan jun shu he Cecembia。4.cao yuan mao chong chang dao hong ji yin zu zhu shi dao yu xin chen dai xie 、tan shui hua ge wu yun shu 、an ji suan ge cheng deng dai xie mi qie xiang guan de ji yin ,biao ming chang dao jun ke neng can yu le cao yuan mao chong de tan shui hua wu dai xie 、wu zhi ge cheng 、wu zhi jiang jie ji xi shou deng sheng li guo cheng ,zai cao yuan mao chong kuo ying gao han cao dian ji duan sheng tai huan jing zhong fa hui chong yao zuo yong 。16Skuo zeng zi ce xu biao ming ,men yuan cao yuan mao chong chang dao jun xiang dui feng du jiao gao de wei Wolbachiahe jia chan bao jun ,er huang ban cao yuan mao chong chang dao jun xiang dui feng du jiao gao de wei rou shi gan jun shu he chang qiu jun 。zhe chong cha yi ke neng yu liang chong cao yuan mao chong qi xi de cha yi xing hai ba huan jing xiang guan 。men yuan cao yuan mao chong chang dao jun αduo yang xing wei xian zhe gao yu huang ban cao yuan mao chong (P>0.05),dan zai bu kao lv chang dao jun xiang dui feng du de qing kuang xia ,bu dong gan jun shu 、mo se gan jun 、ya chan bao jun shu 、shi qing jun shu 、jia yang jun shu 、Wolbachia、chang qiu jun shu 、jia chan bao jun shu he rou shi gan jun shu deng 9ge shu zai liang chong cao yuan mao chong jian cha yi xian zhe (P<0.01)。ju ci tui ce ,zhe xie jun ke neng yu liang chong cao yuan mao chong kuo ying cha yi xing hai ba huan jing xiang guan 。
论文参考文献
论文详细介绍
论文作者分别是来自兰州大学的杨兴卓，发表于刊物兰州大学2019-07-29论文，是一篇关于草原毛虫论文,青藏高原论文,生态适应论文,高寒草甸论文,肠道菌论文,分离培养论文,扩增子论文,宏基因组论文,高通量测序论文,兰州大学2019-07-29论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自兰州大学2019-07-29论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：草原毛虫论文; 青藏高原论文; 生态适应论文; 高寒草甸论文; 肠道菌论文; 分离培养论文; 扩增子论文; 宏基因组论文; 高通量测序论文; 兰州大学2019-07-29论文;