本文主要研究内容
作者何明霞,田甜,刘立媛,步绍翀,东莉洁,张欣欣,张洪桢（2019）在《小鼠小梁细胞与肌成纤维细胞的同步辐射红外显微光谱》一文中研究指出：原发性开角型青光眼是常见的致盲性眼部疾病。眼压升高是原发性开角型青光眼发生和发展最主要的危险因素,是由小梁网途径的房水外流排出系统发生病变、房水流出阻力增加所致。研究表明,房水中存在的转化生长因子-β能够使小梁细胞纤维化,诱导小梁细胞过度增殖,从而阻碍房水外流,导致原发性开角型青光眼的发生。原发性开角型青光眼发病隐蔽,病程进展缓慢,早期没有任何症状,往往到晚期视力视野有显著损害时,才会被发现,因此原发性开角型青光眼的早期诊断尤为重要。同步辐射红外显微成像结合高亮度、高分辨率的同步辐射源,同时配备傅里叶变换红外光谱仪与红外显微镜,可以实现细胞的检测。这对从分子层面获取细胞的变化信息,深入理解疾病的发病机制以及疾病的早期诊断具有非常重要的意义。虽然有很多红外光谱在生物医学领域的研究报道,但是应用红外光谱显微成像技术研究细胞等生物医学体系仍然是亟待发展的领域,并且目前未找到关于红外光谱用于小梁网细胞的检测报道。在体外用转化生长因子-β对老鼠小梁网细胞进行诱导,使其转化为肌成纤维细胞,模拟小梁细胞纤维化过程。对小梁网细胞以及经转化生长因子-β诱导形成的肌成纤维细胞进行同步辐射红外显微成像及光谱分析,并进一步探讨同步辐射用于早期诊断原发性开角型青光眼的可行性。研究表明肌成纤维细胞内的弹性蛋白明显高于小梁网细胞,而弹性蛋白中95%为非极性氨基酸,即氨基酸的侧链基团R基只有C和H两种元素。对比两种细胞的红外谱图,发现在2 934, 2 900和2 845 cm-1,肌成纤维细胞的CH3, CH2和CH的伸缩振动明显强于小梁网细胞,推测可能是由于转化生长因子-β诱导后细胞内弹性蛋白增加所致。在细胞层面检测了小梁网细胞的过度增殖,为将来可以直接获取细胞的红外光谱从而检测小梁网细胞的增殖程度,进而检测原发性开角型青光眼等疾病奠定了基础。得出同步辐射红外谱学与显微成像有望成为检测原发性开角型青光眼新手段的结论,也为将来便携式红外显微光谱仪临床实时检测青光眼等疾病提供了依据。
Abstract
yuan fa xing kai jiao xing qing guang yan shi chang jian de zhi mang xing yan bu ji bing 。yan ya sheng gao shi yuan fa xing kai jiao xing qing guang yan fa sheng he fa zhan zui zhu yao de wei xian yin su ,shi you xiao liang wang tu jing de fang shui wai liu pai chu ji tong fa sheng bing bian 、fang shui liu chu zu li zeng jia suo zhi 。yan jiu biao ming ,fang shui zhong cun zai de zhuai hua sheng chang yin zi -βneng gou shi xiao liang xi bao qian wei hua ,you dao xiao liang xi bao guo du zeng shi ,cong er zu ai fang shui wai liu ,dao zhi yuan fa xing kai jiao xing qing guang yan de fa sheng 。yuan fa xing kai jiao xing qing guang yan fa bing yin bi ,bing cheng jin zhan huan man ,zao ji mei you ren he zheng zhuang ,wang wang dao wan ji shi li shi ye you xian zhe sun hai shi ,cai hui bei fa xian ,yin ci yuan fa xing kai jiao xing qing guang yan de zao ji zhen duan you wei chong yao 。tong bu fu she gong wai xian wei cheng xiang jie ge gao liang du 、gao fen bian lv de tong bu fu she yuan ,tong shi pei bei fu li xie bian huan gong wai guang pu yi yu gong wai xian wei jing ,ke yi shi xian xi bao de jian ce 。zhe dui cong fen zi ceng mian huo qu xi bao de bian hua xin xi ,shen ru li jie ji bing de fa bing ji zhi yi ji ji bing de zao ji zhen duan ju you fei chang chong yao de yi yi 。sui ran you hen duo gong wai guang pu zai sheng wu yi xue ling yu de yan jiu bao dao ,dan shi ying yong gong wai guang pu xian wei cheng xiang ji shu yan jiu xi bao deng sheng wu yi xue ti ji reng ran shi ji dai fa zhan de ling yu ,bing ju mu qian wei zhao dao guan yu gong wai guang pu yong yu xiao liang wang xi bao de jian ce bao dao 。zai ti wai yong zhuai hua sheng chang yin zi -βdui lao shu xiao liang wang xi bao jin hang you dao ,shi ji zhuai hua wei ji cheng qian wei xi bao ,mo ni xiao liang xi bao qian wei hua guo cheng 。dui xiao liang wang xi bao yi ji jing zhuai hua sheng chang yin zi -βyou dao xing cheng de ji cheng qian wei xi bao jin hang tong bu fu she gong wai xian wei cheng xiang ji guang pu fen xi ,bing jin yi bu tan tao tong bu fu she yong yu zao ji zhen duan yuan fa xing kai jiao xing qing guang yan de ke hang xing 。yan jiu biao ming ji cheng qian wei xi bao nei de dan xing dan bai ming xian gao yu xiao liang wang xi bao ,er dan xing dan bai zhong 95%wei fei ji xing an ji suan ,ji an ji suan de ce lian ji tuan Rji zhi you Che Hliang chong yuan su 。dui bi liang chong xi bao de gong wai pu tu ,fa xian zai 2 934, 2 900he 2 845 cm-1,ji cheng qian wei xi bao de CH3, CH2he CHde shen su zhen dong ming xian jiang yu xiao liang wang xi bao ,tui ce ke neng shi you yu zhuai hua sheng chang yin zi -βyou dao hou xi bao nei dan xing dan bai zeng jia suo zhi 。zai xi bao ceng mian jian ce le xiao liang wang xi bao de guo du zeng shi ,wei jiang lai ke yi zhi jie huo qu xi bao de gong wai guang pu cong er jian ce xiao liang wang xi bao de zeng shi cheng du ,jin er jian ce yuan fa xing kai jiao xing qing guang yan deng ji bing dian ding le ji chu 。de chu tong bu fu she gong wai pu xue yu xian wei cheng xiang you wang cheng wei jian ce yuan fa xing kai jiao xing qing guang yan xin shou duan de jie lun ,ye wei jiang lai bian xie shi gong wai xian wei guang pu yi lin chuang shi shi jian ce qing guang yan deng ji bing di gong le yi ju 。
论文参考文献
论文详细介绍
论文作者分别是来自光谱学与光谱分析的何明霞,田甜,刘立媛,步绍翀,东莉洁,张欣欣,张洪桢，发表于刊物光谱学与光谱分析2019年11期论文，是一篇关于同步辐射红外显微成像论文,原发性开角型青光眼论文,小梁网细胞论文,肌成纤维细胞论文,弹性蛋白论文,光谱学与光谱分析2019年11期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自光谱学与光谱分析2019年11期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：同步辐射红外显微成像论文; 原发性开角型青光眼论文; 小梁网细胞论文; 肌成纤维细胞论文; 弹性蛋白论文; 光谱学与光谱分析2019年11期论文;