本文主要研究内容
作者沙月霞,隋书婷,曾庆超,沈瑞清（2019）在《贝莱斯芽孢杆菌E69预防稻瘟病等多种真菌病害的潜力》一文中研究指出：【目的】明确水稻内生菌贝莱斯芽孢杆菌(Bacillus velezensis)菌株E69对多种植物病原真菌的拮抗作用,尤其是对稻瘟病的生物防治效果,减少化学农药的使用。【方法】贝莱斯芽孢杆菌菌株E69和枯草芽孢杆菌(B. subtilis,稻瘟病生物防治最常用微生物)菌株E66分离于水稻叶片内生细菌,采用对峙培养法测试菌株E69和E66及其发酵液、无菌上清液对稻瘟病菌(Magnaporthe oryzae)的拮抗作用,并测试两株内生芽孢杆菌对立枯丝核菌(Rhizoctonia solani)、镰孢菌(Fusarium spp.)、番茄灰霉病菌(Botrytis cinerea)、草莓炭疽病菌(Colletotrichum gloeospoioides)、烟草黑胫病菌(Phytophthora parasitica var. nicotianae)、链格孢菌(Alternaria alternate)、西瓜枯萎病菌(F. oxysporum)等11种植物病原菌的拮抗作用,温室条件下检测对水稻叶瘟的预防效果,田间试验检测对水稻叶瘟和穗颈瘟的预防效果,常规抑菌测试法研究对稻瘟病菌分生孢子萌发和附着胞形成的抑制作用。采用激光共聚焦显微镜观察绿色荧光蛋白标记后的工程菌株E69在水稻茎部的定殖情况。【结果】菌株E69和E66对稻瘟病菌菌丝生长具有显著拮抗作用,温室条件下两个菌株对稻瘟病的预防效果分别为83.24%和76.57%,对叶瘟的田间预防效果分别为85.97%和79.76%,对穗颈瘟的田间预防效果分别为69.67%和68.82%,E69对叶瘟的预防效果显著高于75%的三环唑可湿性粉剂,对穗颈瘟的预防效果与三环唑无显著差异。菌株E69和E66对立枯丝核菌、镰孢菌、番茄灰霉病菌、草莓炭疽病菌、烟草黑胫病菌、叶枯病菌、西瓜枯萎病菌等11种植物病原菌有显著拮抗作用,E69的拮抗作用明显高于E66。菌株E69和E66能够强烈抑制稻瘟病菌分生孢子萌发和附着胞的形成,E69发酵液的抑菌效果分别为95.28%和94.16%,无菌上清液的抑菌效果分别为85.36%和84.31%;E66发酵液对分生孢子萌发和附着胞形成的抑菌效果分别为89.15%和87.38%,无菌上清液的抑菌效果分别为79.65%和72.45%。绿色荧光蛋白GFP78标记后的工程菌株E69在水稻茎部具有较好的定殖能力,可以稳定定殖在水稻茎部表皮、薄壁组织和维管束。【结论】贝莱斯芽孢杆菌菌株E69是一种潜在的、预防效果明显的生防菌株,具有预防稻瘟病兼防纹枯病等多种真菌病害的应用潜力。
Abstract
【mu de 】ming que shui dao nei sheng jun bei lai si ya bao gan jun (Bacillus velezensis)jun zhu E69dui duo chong zhi wu bing yuan zhen jun de jie kang zuo yong ,you ji shi dui dao wen bing de sheng wu fang zhi xiao guo ,jian shao hua xue nong yao de shi yong 。【fang fa 】bei lai si ya bao gan jun jun zhu E69he ku cao ya bao gan jun (B. subtilis,dao wen bing sheng wu fang zhi zui chang yong wei sheng wu )jun zhu E66fen li yu shui dao xie pian nei sheng xi jun ,cai yong dui shi pei yang fa ce shi jun zhu E69he E66ji ji fa jiao ye 、mo jun shang qing ye dui dao wen bing jun (Magnaporthe oryzae)de jie kang zuo yong ,bing ce shi liang zhu nei sheng ya bao gan jun dui li ku si he jun (Rhizoctonia solani)、lian bao jun (Fusarium spp.)、fan jia hui mei bing jun (Botrytis cinerea)、cao mei tan ju bing jun (Colletotrichum gloeospoioides)、yan cao hei jing bing jun (Phytophthora parasitica var. nicotianae)、lian ge bao jun (Alternaria alternate)、xi gua ku wei bing jun (F. oxysporum)deng 11chong zhi wu bing yuan jun de jie kang zuo yong ,wen shi tiao jian xia jian ce dui shui dao xie wen de yu fang xiao guo ,tian jian shi yan jian ce dui shui dao xie wen he sui geng wen de yu fang xiao guo ,chang gui yi jun ce shi fa yan jiu dui dao wen bing jun fen sheng bao zi meng fa he fu zhao bao xing cheng de yi zhi zuo yong 。cai yong ji guang gong ju jiao xian wei jing guan cha lu se ying guang dan bai biao ji hou de gong cheng jun zhu E69zai shui dao jing bu de ding shi qing kuang 。【jie guo 】jun zhu E69he E66dui dao wen bing jun jun si sheng chang ju you xian zhe jie kang zuo yong ,wen shi tiao jian xia liang ge jun zhu dui dao wen bing de yu fang xiao guo fen bie wei 83.24%he 76.57%,dui xie wen de tian jian yu fang xiao guo fen bie wei 85.97%he 79.76%,dui sui geng wen de tian jian yu fang xiao guo fen bie wei 69.67%he 68.82%,E69dui xie wen de yu fang xiao guo xian zhe gao yu 75%de san huan zuo ke shi xing fen ji ,dui sui geng wen de yu fang xiao guo yu san huan zuo mo xian zhe cha yi 。jun zhu E69he E66dui li ku si he jun 、lian bao jun 、fan jia hui mei bing jun 、cao mei tan ju bing jun 、yan cao hei jing bing jun 、xie ku bing jun 、xi gua ku wei bing jun deng 11chong zhi wu bing yuan jun you xian zhe jie kang zuo yong ,E69de jie kang zuo yong ming xian gao yu E66。jun zhu E69he E66neng gou jiang lie yi zhi dao wen bing jun fen sheng bao zi meng fa he fu zhao bao de xing cheng ,E69fa jiao ye de yi jun xiao guo fen bie wei 95.28%he 94.16%,mo jun shang qing ye de yi jun xiao guo fen bie wei 85.36%he 84.31%;E66fa jiao ye dui fen sheng bao zi meng fa he fu zhao bao xing cheng de yi jun xiao guo fen bie wei 89.15%he 87.38%,mo jun shang qing ye de yi jun xiao guo fen bie wei 79.65%he 72.45%。lu se ying guang dan bai GFP78biao ji hou de gong cheng jun zhu E69zai shui dao jing bu ju you jiao hao de ding shi neng li ,ke yi wen ding ding shi zai shui dao jing bu biao pi 、bao bi zu zhi he wei guan shu 。【jie lun 】bei lai si ya bao gan jun jun zhu E69shi yi chong qian zai de 、yu fang xiao guo ming xian de sheng fang jun zhu ,ju you yu fang dao wen bing jian fang wen ku bing deng duo chong zhen jun bing hai de ying yong qian li 。
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论文详细介绍
论文作者分别是来自中国农业科学的沙月霞,隋书婷,曾庆超,沈瑞清，发表于刊物中国农业科学2019年11期论文，是一篇关于贝莱斯芽孢杆菌论文,稻瘟病论文,植物病原真菌论文,生物防治论文,定殖能力论文,中国农业科学2019年11期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自中国农业科学2019年11期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：贝莱斯芽孢杆菌论文; 稻瘟病论文; 植物病原真菌论文; 生物防治论文; 定殖能力论文; 中国农业科学2019年11期论文;