:Study on Rapid Detection of Tetracyclines,Fluoroquinolones and Sulfonamides in Milk论文

:Study on Rapid Detection of Tetracyclines,Fluoroquinolones and Sulfonamides in Milk论文

本文主要研究内容

作者(2019)在《Study on Rapid Detection of Tetracyclines,Fluoroquinolones and Sulfonamides in Milk》一文中研究指出:Aiming at the market demand for rapid detection of tetracyclines,fluoroquinolones and sulfonamides in milk,a golloidal gold immunochromatography test strip for simultaneous detection of tetracyclines,fluoroquinolones and sulfonamides in milk was prepared based on the principle of competitive inhibition immunochromatography. The performance indicators of the test strip were verified. The results showed that the test strip can simultaneously detect 4 tetracyclines,13 fluoroquinolones and 13 sulfonamides,and the detection limits all can meet the national residue limits; the tests strip exhibited false positive rate≤5% and false negative rate = 0; and no cross-reaction with other drugs was commonly found in milk,indicating good specificity. The method is simple,rapid,and has low cost and easy popularization. It provides a means for realizing on-site rapid detection and is of important practical significance to guarantee of safety of milk and dairy products in China.

Abstract

Aiming at the market demand for rapid detection of tetracyclines,fluoroquinolones and sulfonamides in milk,a golloidal gold immunochromatography test strip for simultaneous detection of tetracyclines,fluoroquinolones and sulfonamides in milk was prepared based on the principle of competitive inhibition immunochromatography. The performance indicators of the test strip were verified. The results showed that the test strip can simultaneously detect 4 tetracyclines,13 fluoroquinolones and 13 sulfonamides,and the detection limits all can meet the national residue limits; the tests strip exhibited false positive rate≤5% and false negative rate = 0; and no cross-reaction with other drugs was commonly found in milk,indicating good specificity. The method is simple,rapid,and has low cost and easy popularization. It provides a means for realizing on-site rapid detection and is of important practical significance to guarantee of safety of milk and dairy products in China.

论文参考文献

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  • [2].An Immunochromatographic Strip Test for Rapid Detection of Antibodies against Porcine Circovirus Type 2[J]. ZHANG Wen-tong1,2, WEI Feng1,3, WANG Jin-liang1,3, XIAO Yue-qiang1,3, SHEN Zhi-qiang1,2,3* 1. Shandong Research & Development Center of Veterinary Propolis Vaccines, Binzhou 256600, China; 2. Shandong Lvdu Biological Technology Co., Ltd., Binzhou 256600, China; 3. Shandong Binzhou Animal Science & Veterinary Medicine Academy, Binzhou 256600, China.  Animal Husbandry and Feed Science.2011(05)
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  • 论文详细介绍

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