本文主要研究内容
作者任雪羽（2019）在《木槿种子的秋水仙素和EMS诱变与鉴定》一文中研究指出：木槿(Hibiscussyriacus Linn.),锦葵科(Malvaceae)木槿属(Hibiscus)落叶灌木,是优良的园林观花树种,兼具食用和药用价值。针对国内木槿种质创新及育种工作起步较晚现象,以木槿种子为材料,通过秋水仙素和EMS浸渍法,探索不同影响因素下的最佳诱变组合。对诱变株进行形态学、细胞学及分子学鉴定,筛选出变异植株;再进行生理指标测定,预测变异株的抗性变化方向。从而为木槿园林应用提供创新材料。同时,从分子学角度为木槿在基因遗传分析、基因定位与图位克隆方面奠定基础。主要研究结果如下:木槿秋水仙素诱变育种中,以清水浸泡0h时木槿种子的发芽率最高,达65%。结合种子的致死率、成苗率及变异率,确定种子萌发2天后,0.2%秋水仙素浸泡12h为秋水仙素诱导木槿种子变异的最佳组合。诱变株出现株高矮化、茎段增粗、节间距缩短、叶基角α及叶片的长宽比增大、叶片退绿黄化和皱缩等变异现象。叶片气孔形态差异显著,细胞呈现多倍体的巨大性;气孔数目相对减少55.90%,气孔密度明显下降。流式细胞术显示细胞核DNA含量随着染色体数目的成倍增加而增加。从形态学和细胞学鉴定上能够快速准确的鉴定出有效变异。变异株叶绿体色素含量随染色体数目的增加明显上升,叶片表现为深绿色;变异株细胞电解质渗出率随着倍性的增加逐渐降低,推测秋水仙素诱变加倍后,植株抗逆性有所增强。木槿EMS诱变育种中,以种子萌发2天后,0.2%EMS浸泡12h为EMS诱导木槿种子变异的最佳组合。诱变株出现了矮化、增粗、节间缩短,叶片退绿黄化、卷曲、叶脉明显等变异现象。在分子学上,建立了木槿ISSR-PCR(25μl)最佳反应体系:10×buffer(Mg2+ free)2.5μl、Tag酶0.75U、Mg2+ 2mmol/L、dNTPs0.1mmol/L、模板DNA 100ng、引物0.5μnol/L,退火温度50.0℃。筛选出6条引物的多态带百分率为95.08%。在ISSR-PCR扩增中,诱变株条带表现为缺失位点,新增位点,既缺失又新增3种情况。遗传相似性(SM)平均值为0.885,平均遗传距离(GD)为0.142。UPGMA聚类结果在遗传距离系数0.85时,将诱变株分为三大类,与形态学初步分类的三大类存在一致性,表明ISSR-PCR分子鉴定法是对木槿EMS诱变的可靠鉴定手段。变异株中叶绿体色素含量对应叶色的深浅变化;变异株的细胞电解质渗出率大多上升,推测经过EMS诱变后,植株抗性有所减弱。
Abstract
mu jin (Hibiscussyriacus Linn.),jin kui ke (Malvaceae)mu jin shu (Hibiscus)la xie guan mu ,shi you liang de yuan lin guan hua shu chong ,jian ju shi yong he yao yong jia zhi 。zhen dui guo nei mu jin chong zhi chuang xin ji yo chong gong zuo qi bu jiao wan xian xiang ,yi mu jin chong zi wei cai liao ,tong guo qiu shui xian su he EMSjin zi fa ,tan suo bu tong ying xiang yin su xia de zui jia you bian zu ge 。dui you bian zhu jin hang xing tai xue 、xi bao xue ji fen zi xue jian ding ,shai shua chu bian yi zhi zhu ;zai jin hang sheng li zhi biao ce ding ,yu ce bian yi zhu de kang xing bian hua fang xiang 。cong er wei mu jin yuan lin ying yong di gong chuang xin cai liao 。tong shi ,cong fen zi xue jiao du wei mu jin zai ji yin wei chuan fen xi 、ji yin ding wei yu tu wei ke long fang mian dian ding ji chu 。zhu yao yan jiu jie guo ru xia :mu jin qiu shui xian su you bian yo chong zhong ,yi qing shui jin pao 0hshi mu jin chong zi de fa ya lv zui gao ,da 65%。jie ge chong zi de zhi si lv 、cheng miao lv ji bian yi lv ,que ding chong zi meng fa 2tian hou ,0.2%qiu shui xian su jin pao 12hwei qiu shui xian su you dao mu jin chong zi bian yi de zui jia zu ge 。you bian zhu chu xian zhu gao ai hua 、jing duan zeng cu 、jie jian ju su duan 、xie ji jiao αji xie pian de chang kuan bi zeng da 、xie pian tui lu huang hua he zhou su deng bian yi xian xiang 。xie pian qi kong xing tai cha yi xian zhe ,xi bao cheng xian duo bei ti de ju da xing ;qi kong shu mu xiang dui jian shao 55.90%,qi kong mi du ming xian xia jiang 。liu shi xi bao shu xian shi xi bao he DNAhan liang sui zhao ran se ti shu mu de cheng bei zeng jia er zeng jia 。cong xing tai xue he xi bao xue jian ding shang neng gou kuai su zhun que de jian ding chu you xiao bian yi 。bian yi zhu xie lu ti se su han liang sui ran se ti shu mu de zeng jia ming xian shang sheng ,xie pian biao xian wei shen lu se ;bian yi zhu xi bao dian jie zhi shen chu lv sui zhao bei xing de zeng jia zhu jian jiang di ,tui ce qiu shui xian su you bian jia bei hou ,zhi zhu kang ni xing you suo zeng jiang 。mu jin EMSyou bian yo chong zhong ,yi chong zi meng fa 2tian hou ,0.2%EMSjin pao 12hwei EMSyou dao mu jin chong zi bian yi de zui jia zu ge 。you bian zhu chu xian le ai hua 、zeng cu 、jie jian su duan ,xie pian tui lu huang hua 、juan qu 、xie mai ming xian deng bian yi xian xiang 。zai fen zi xue shang ,jian li le mu jin ISSR-PCR(25μl)zui jia fan ying ti ji :10×buffer(Mg2+ free)2.5μl、Tagmei 0.75U、Mg2+ 2mmol/L、dNTPs0.1mmol/L、mo ban DNA 100ng、yin wu 0.5μnol/L,tui huo wen du 50.0℃。shai shua chu 6tiao yin wu de duo tai dai bai fen lv wei 95.08%。zai ISSR-PCRkuo zeng zhong ,you bian zhu tiao dai biao xian wei que shi wei dian ,xin zeng wei dian ,ji que shi you xin zeng 3chong qing kuang 。wei chuan xiang shi xing (SM)ping jun zhi wei 0.885,ping jun wei chuan ju li (GD)wei 0.142。UPGMAju lei jie guo zai wei chuan ju li ji shu 0.85shi ,jiang you bian zhu fen wei san da lei ,yu xing tai xue chu bu fen lei de san da lei cun zai yi zhi xing ,biao ming ISSR-PCRfen zi jian ding fa shi dui mu jin EMSyou bian de ke kao jian ding shou duan 。bian yi zhu zhong xie lu ti se su han liang dui ying xie se de shen jian bian hua ;bian yi zhu de xi bao dian jie zhi shen chu lv da duo shang sheng ,tui ce jing guo EMSyou bian hou ,zhi zhu kang xing you suo jian ruo 。
论文参考文献
论文详细介绍
论文作者分别是来自中南林业科技大学的任雪羽，发表于刊物中南林业科技大学2019-09-29论文，是一篇关于木槿论文,诱变育种论文,秋水仙素论文,流式细胞术论文,中南林业科技大学2019-09-29论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自中南林业科技大学2019-09-29论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：木槿论文; 诱变育种论文; 秋水仙素论文; 流式细胞术论文; 中南林业科技大学2019-09-29论文;