本文主要研究内容
作者卢美欢,李利军,马英辉,王晓兵,郭邦利,王晓娥（2019）在《魔芋软腐病病原菌TaqMan荧光探针PCR技术的建立及应用》一文中研究指出：为实现对田间土壤软腐病病原菌的定量检测,基于魔芋软腐病优势病原菌胡萝卜软腐果胶杆菌胡萝卜亚种Pectobacterium carotovorum subsp. carotovorum的FyuA基因序列,设计特异性引物PCC1/PCC2/PCC3,建立TaqMan荧光探针实时荧光定量PCR技术,并对魔芋根系土壤中软腐病病原菌进行动态监测。结果显示:基于FyuA基因序列设计的引物特异性好,仅能特异性检出胡萝卜软腐果胶杆菌胡萝卜亚种;当模拟带菌土壤中病原菌浓度低至1.88 CFU/g时也能检出,灵敏度高;发病魔芋根际土壤中软腐病病原菌检出率为100.00%,病原菌DNA浓度最高达到了7.52×10~7ng/μL,健康魔芋根际土壤中也存在病原菌,检出率为40.00%;不同种植模式中,林下魔芋土壤中软腐病病原菌数量较少;连作时间与病原菌数量、病情指数存在正相关关系,连作时间越长,病原菌积累越多,魔芋病情指数也越高,魔芋连作4年土壤中病原菌DNA浓度最高达到4.03×10~4ng/μL;对魔芋土壤软腐病病原菌进行全年监测,病原菌数量随着月份增长逐渐上升,在8—10月达到峰值543.20 ng/μL后下降,病原菌数量与魔芋病情指数变化规律一致,但田间魔芋软腐病的发生相对滞后。表明建立的TaqMan荧光探针实时荧光定量PCR技术可用于田间魔芋软腐病的监测。
Abstract
wei shi xian dui tian jian tu rang ruan fu bing bing yuan jun de ding liang jian ce ,ji yu mo yu ruan fu bing you shi bing yuan jun hu luo bo ruan fu guo jiao gan jun hu luo bo ya chong Pectobacterium carotovorum subsp. carotovorumde FyuAji yin xu lie ,she ji te yi xing yin wu PCC1/PCC2/PCC3,jian li TaqManying guang tan zhen shi shi ying guang ding liang PCRji shu ,bing dui mo yu gen ji tu rang zhong ruan fu bing bing yuan jun jin hang dong tai jian ce 。jie guo xian shi :ji yu FyuAji yin xu lie she ji de yin wu te yi xing hao ,jin neng te yi xing jian chu hu luo bo ruan fu guo jiao gan jun hu luo bo ya chong ;dang mo ni dai jun tu rang zhong bing yuan jun nong du di zhi 1.88 CFU/gshi ye neng jian chu ,ling min du gao ;fa bing mo yu gen ji tu rang zhong ruan fu bing bing yuan jun jian chu lv wei 100.00%,bing yuan jun DNAnong du zui gao da dao le 7.52×10~7ng/μL,jian kang mo yu gen ji tu rang zhong ye cun zai bing yuan jun ,jian chu lv wei 40.00%;bu tong chong zhi mo shi zhong ,lin xia mo yu tu rang zhong ruan fu bing bing yuan jun shu liang jiao shao ;lian zuo shi jian yu bing yuan jun shu liang 、bing qing zhi shu cun zai zheng xiang guan guan ji ,lian zuo shi jian yue chang ,bing yuan jun ji lei yue duo ,mo yu bing qing zhi shu ye yue gao ,mo yu lian zuo 4nian tu rang zhong bing yuan jun DNAnong du zui gao da dao 4.03×10~4ng/μL;dui mo yu tu rang ruan fu bing bing yuan jun jin hang quan nian jian ce ,bing yuan jun shu liang sui zhao yue fen zeng chang zhu jian shang sheng ,zai 8—10yue da dao feng zhi 543.20 ng/μLhou xia jiang ,bing yuan jun shu liang yu mo yu bing qing zhi shu bian hua gui lv yi zhi ,dan tian jian mo yu ruan fu bing de fa sheng xiang dui zhi hou 。biao ming jian li de TaqManying guang tan zhen shi shi ying guang ding liang PCRji shu ke yong yu tian jian mo yu ruan fu bing de jian ce 。
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论文作者分别是来自植物保护学报的卢美欢,李利军,马英辉,王晓兵,郭邦利,王晓娥，发表于刊物植物保护学报2019年05期论文，是一篇关于魔芋论文,软腐病论文,监测论文,植物保护学报2019年05期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自植物保护学报2019年05期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：魔芋论文; 软腐病论文; 监测论文; 植物保护学报2019年05期论文;