本文主要研究内容
作者朱健树（2019）在《利用玉米秸秆水解液制备生物柴油》一文中研究指出：单细胞油脂是制备生物柴油的重要组成部分,尽管生物柴油的应用范围广阔,但其较高的发酵生产成本限制了其商业化生产。为了有效降低原材料成本,实现廉价生物质材料的工业转换,基于生物质资源的经济型发酵工艺为研究目的,以生物柴油生产菌产油掷孢酵母,为出发菌株,对该菌株进行了一系列的菌种诱变及廉价碳源为底物的发酵条件优化研究。基于微生物油脂发酵生物质资源开发,讨论了玉米秸秆水解液中抑制剂对于油脂合成代谢过程中的影响,旨在为单细胞油脂的选育和廉价碳源的利用提供理论依据。具体操作与结论如下:1.考察优化诱变方法,比较紫外诱变分别与NTG和DES复合诱变,再分别采用马来酰肼和浅蓝菌素进行初筛,最后摇瓶发酵进行复筛。结果表明:当紫外照射时间30 s,DES诱变时间100 min,浅蓝菌素浓度17.9μmol/L时,突变株生物量,含油率及油脂产量较原始菌株分别提高了42.2%,60.0%,127.8%,说明此种复合诱变及筛选方法可以有效提高菌体的产油性能,且诱变后,不饱和脂肪酸的含量升高,二十碳脂肪酸的含量降低,说明油脂的低温流动性较原始菌株有所提高。2.考察混合碳源发酵对产油的影响。分别以木糖、葡萄糖和甘油为唯一碳源进行发酵产油,作为混糖对照结果,实验证明以葡萄糖为唯一碳源时发酵性能最优,生物量为30.7 g/L,含油率27.1%,油脂产量8.3 g/L。混碳发酵中,以葡萄糖和木糖为混合碳源,初糖浓度为3:1时,生物量27.3 g/L,含油率28.6%,油脂产量7.8 g/L;而以葡萄糖、甘油为混合碳源时,碳源比例对发酵结果影响不大,目标产物的收获量优于以甘油为唯一碳源时的产量;以木糖和甘油为混合碳源时,生物量急剧下降,最低为15.3 g/L,造成目的产物收获量不足,仅为2.9 g/L。3.考察玉米秸秆水解液中抑制剂对于菌株产油的影响。以糠醛为抑制剂时,在木糖为唯一碳源的培养基中,较低浓度的糠醛对菌株抑制明显,0.5 g/L时,菌体干重较对照降低了27.5%,含油率降低了13.9%,油脂产量降低了37.7%;而在含葡萄糖培养基中,糠醛浓度达到1.0 g/L才出现明显抑制,证明在葡萄糖培养基中菌株对糠醛的耐受性相较木糖培养基强。考察苯酚对菌株的抑制情况时发现,高浓度苯酚对菌株抑制明显,木糖培养基中菌株对苯酚的耐受性高于葡萄糖培养基;而低浓度苯酚(0.2 g/L),对木糖培养基则产生正效应。以乙酸为抑制剂时,0.9 g/L乙酸对木糖培养基中的菌株产生显著抑制;当其为1.2 g/L时,葡萄糖培养基中的菌株活力较低,生物量、含油率、油脂产量分别降低了29.6%、39.6%、55.9%。4.考察不同处理程度的玉米秸秆水解液的发酵能力。在玉米秸秆稀酸水解液中菌体的生物量、含油率和油脂产量分别为23.2 g/L,18.9%和4.4 g/L;在完全水解液中,经考察,最佳发酵时间为144 h,此时生物量为24.4 g/L,含油率达22.3%,油脂产量至5.4 g/L。
Abstract
chan xi bao you zhi shi zhi bei sheng wu chai you de chong yao zu cheng bu fen ,jin guan sheng wu chai you de ying yong fan wei an kuo ,dan ji jiao gao de fa jiao sheng chan cheng ben xian zhi le ji shang ye hua sheng chan 。wei le you xiao jiang di yuan cai liao cheng ben ,shi xian lian jia sheng wu zhi cai liao de gong ye zhuai huan ,ji yu sheng wu zhi zi yuan de jing ji xing fa jiao gong yi wei yan jiu mu de ,yi sheng wu chai you sheng chan jun chan you zhi bao jiao mu ,wei chu fa jun zhu ,dui gai jun zhu jin hang le yi ji lie de jun chong you bian ji lian jia tan yuan wei de wu de fa jiao tiao jian you hua yan jiu 。ji yu wei sheng wu you zhi fa jiao sheng wu zhi zi yuan kai fa ,tao lun le yu mi jie gan shui jie ye zhong yi zhi ji dui yu you zhi ge cheng dai xie guo cheng zhong de ying xiang ,zhi zai wei chan xi bao you zhi de shua yo he lian jia tan yuan de li yong di gong li lun yi ju 。ju ti cao zuo yu jie lun ru xia :1.kao cha you hua you bian fang fa ,bi jiao zi wai you bian fen bie yu NTGhe DESfu ge you bian ,zai fen bie cai yong ma lai xian jing he jian lan jun su jin hang chu shai ,zui hou yao ping fa jiao jin hang fu shai 。jie guo biao ming :dang zi wai zhao she shi jian 30 s,DESyou bian shi jian 100 min,jian lan jun su nong du 17.9μmol/Lshi ,tu bian zhu sheng wu liang ,han you lv ji you zhi chan liang jiao yuan shi jun zhu fen bie di gao le 42.2%,60.0%,127.8%,shui ming ci chong fu ge you bian ji shai shua fang fa ke yi you xiao di gao jun ti de chan you xing neng ,ju you bian hou ,bu bao he zhi fang suan de han liang sheng gao ,er shi tan zhi fang suan de han liang jiang di ,shui ming you zhi de di wen liu dong xing jiao yuan shi jun zhu you suo di gao 。2.kao cha hun ge tan yuan fa jiao dui chan you de ying xiang 。fen bie yi mu tang 、pu tao tang he gan you wei wei yi tan yuan jin hang fa jiao chan you ,zuo wei hun tang dui zhao jie guo ,shi yan zheng ming yi pu tao tang wei wei yi tan yuan shi fa jiao xing neng zui you ,sheng wu liang wei 30.7 g/L,han you lv 27.1%,you zhi chan liang 8.3 g/L。hun tan fa jiao zhong ,yi pu tao tang he mu tang wei hun ge tan yuan ,chu tang nong du wei 3:1shi ,sheng wu liang 27.3 g/L,han you lv 28.6%,you zhi chan liang 7.8 g/L;er yi pu tao tang 、gan you wei hun ge tan yuan shi ,tan yuan bi li dui fa jiao jie guo ying xiang bu da ,mu biao chan wu de shou huo liang you yu yi gan you wei wei yi tan yuan shi de chan liang ;yi mu tang he gan you wei hun ge tan yuan shi ,sheng wu liang ji ju xia jiang ,zui di wei 15.3 g/L,zao cheng mu de chan wu shou huo liang bu zu ,jin wei 2.9 g/L。3.kao cha yu mi jie gan shui jie ye zhong yi zhi ji dui yu jun zhu chan you de ying xiang 。yi kang quan wei yi zhi ji shi ,zai mu tang wei wei yi tan yuan de pei yang ji zhong ,jiao di nong du de kang quan dui jun zhu yi zhi ming xian ,0.5 g/Lshi ,jun ti gan chong jiao dui zhao jiang di le 27.5%,han you lv jiang di le 13.9%,you zhi chan liang jiang di le 37.7%;er zai han pu tao tang pei yang ji zhong ,kang quan nong du da dao 1.0 g/Lcai chu xian ming xian yi zhi ,zheng ming zai pu tao tang pei yang ji zhong jun zhu dui kang quan de nai shou xing xiang jiao mu tang pei yang ji jiang 。kao cha ben fen dui jun zhu de yi zhi qing kuang shi fa xian ,gao nong du ben fen dui jun zhu yi zhi ming xian ,mu tang pei yang ji zhong jun zhu dui ben fen de nai shou xing gao yu pu tao tang pei yang ji ;er di nong du ben fen (0.2 g/L),dui mu tang pei yang ji ze chan sheng zheng xiao ying 。yi yi suan wei yi zhi ji shi ,0.9 g/Lyi suan dui mu tang pei yang ji zhong de jun zhu chan sheng xian zhe yi zhi ;dang ji wei 1.2 g/Lshi ,pu tao tang pei yang ji zhong de jun zhu huo li jiao di ,sheng wu liang 、han you lv 、you zhi chan liang fen bie jiang di le 29.6%、39.6%、55.9%。4.kao cha bu tong chu li cheng du de yu mi jie gan shui jie ye de fa jiao neng li 。zai yu mi jie gan xi suan shui jie ye zhong jun ti de sheng wu liang 、han you lv he you zhi chan liang fen bie wei 23.2 g/L,18.9%he 4.4 g/L;zai wan quan shui jie ye zhong ,jing kao cha ,zui jia fa jiao shi jian wei 144 h,ci shi sheng wu liang wei 24.4 g/L,han you lv da 22.3%,you zhi chan liang zhi 5.4 g/L。
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论文作者分别是来自长春工业大学的朱健树，发表于刊物长春工业大学2019-09-04论文，是一篇关于生物柴油论文,玉米秸秆论文,复合诱变论文,混合碳源论文,抑制剂论文,长春工业大学2019-09-04论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自长春工业大学2019-09-04论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：生物柴油论文; 玉米秸秆论文; 复合诱变论文; 混合碳源论文; 抑制剂论文; 长春工业大学2019-09-04论文;