本文主要研究内容
作者胡珊,黄皓,梁卫驱,罗华建,陈仕丽,徐匆,钟鸣（2019）在《荔枝内生乳酸菌的筛选、鉴定及发酵效果分析》一文中研究指出：【目的】筛选用于果汁发酵的荔枝内生乳酸菌,为解决发酵果汁专用乳酸菌少的问题提供新思路。【方法】以荔枝果肉为试验材料,通过MRS-溴甲酚紫平板法分离筛选荔枝内生乳酸菌,测定初筛菌株的发酵产酸和生长能力,筛选出发酵性能最优菌株,并进行形态学观察、生理生化试验和16S rDNA鉴定;以常用果蔬汁发酵菌种植物乳杆菌为对照菌株,进行荔枝内生菌发酵荔枝果汁的感官评价、微生物、理化指标和营养物质含量研究。【结果】从荔枝果肉中筛选出1株发酵性能优良的荔枝内生菌(编号LZ1),该菌菌落呈乳白色圆形凸起,边缘整齐,革兰氏阳性细菌,菌体细胞呈球形或卵圆形,单个、成对或链状排列;厌氧生长,能发酵纤维二糖、葡萄糖、果糖等8种碳水化合物;16S rDNA鉴定结果表明该菌与肠膜明串珠菌葡聚糖亚种(Leuconostoc mesenteroides subsp. dextranicum)的同源性达99%以上,结合形态特征及生理生化试验结果,鉴定该菌株为肠膜明串珠菌葡聚糖亚种。该荔枝内生肠膜明串珠菌能在荔枝果汁中生长良好,发酵果汁无分层现象,有浓郁的荔枝清香味,感官评分显著高于对照菌植物乳杆菌(P<0.05,下同),活菌数达4.85×109CFU/mL,高于植物乳杆菌,但差异不显著(P>0.05);肠膜明串珠菌和植物乳杆菌发酵果汁的乳酸含量(9.63和13.83 g/kg)显著高于不接菌果汁(0.44 g/kg),pH(3.73和3.54)显著低于不接菌果汁(4.68);荔枝内生肠膜明串珠菌发酵果汁中的维生素(Vc)含量高于植物乳杆菌和不接菌果汁,对果汁中果糖和葡萄糖的利用能力也强于植物乳杆菌,两株菌发酵果汁的葡萄糖含量分别较不接菌果汁下降31.4%和26.7%,果糖含量降幅分别为43.4%和21.7%;荔枝内生肠膜明串珠菌发酵果汁中游离氨基酸保留率为87.3%,略高于植物乳杆菌(84.4%),两株菌在发酵过程中各种氨基酸的种类和含量变化有所不同。【结论】筛选的荔枝内生肠膜明串珠菌在荔枝果汁中生长良好,发酵的荔枝果汁既保存和优化果汁的营养和口感,又增添益生菌的保健功能。
Abstract
【mu de 】shai shua yong yu guo zhi fa jiao de li zhi nei sheng ru suan jun ,wei jie jue fa jiao guo zhi zhuan yong ru suan jun shao de wen ti di gong xin sai lu 。【fang fa 】yi li zhi guo rou wei shi yan cai liao ,tong guo MRS-xiu jia fen zi ping ban fa fen li shai shua li zhi nei sheng ru suan jun ,ce ding chu shai jun zhu de fa jiao chan suan he sheng chang neng li ,shai shua chu fa jiao xing neng zui you jun zhu ,bing jin hang xing tai xue guan cha 、sheng li sheng hua shi yan he 16S rDNAjian ding ;yi chang yong guo shu zhi fa jiao jun chong zhi wu ru gan jun wei dui zhao jun zhu ,jin hang li zhi nei sheng jun fa jiao li zhi guo zhi de gan guan ping jia 、wei sheng wu 、li hua zhi biao he ying yang wu zhi han liang yan jiu 。【jie guo 】cong li zhi guo rou zhong shai shua chu 1zhu fa jiao xing neng you liang de li zhi nei sheng jun (bian hao LZ1),gai jun jun la cheng ru bai se yuan xing tu qi ,bian yuan zheng ji ,ge lan shi yang xing xi jun ,jun ti xi bao cheng qiu xing huo luan yuan xing ,chan ge 、cheng dui huo lian zhuang pai lie ;ya yang sheng chang ,neng fa jiao qian wei er tang 、pu tao tang 、guo tang deng 8chong tan shui hua ge wu ;16S rDNAjian ding jie guo biao ming gai jun yu chang mo ming chuan zhu jun pu ju tang ya chong (Leuconostoc mesenteroides subsp. dextranicum)de tong yuan xing da 99%yi shang ,jie ge xing tai te zheng ji sheng li sheng hua shi yan jie guo ,jian ding gai jun zhu wei chang mo ming chuan zhu jun pu ju tang ya chong 。gai li zhi nei sheng chang mo ming chuan zhu jun neng zai li zhi guo zhi zhong sheng chang liang hao ,fa jiao guo zhi mo fen ceng xian xiang ,you nong yu de li zhi qing xiang wei ,gan guan ping fen xian zhe gao yu dui zhao jun zhi wu ru gan jun (P<0.05,xia tong ),huo jun shu da 4.85×109CFU/mL,gao yu zhi wu ru gan jun ,dan cha yi bu xian zhe (P>0.05);chang mo ming chuan zhu jun he zhi wu ru gan jun fa jiao guo zhi de ru suan han liang (9.63he 13.83 g/kg)xian zhe gao yu bu jie jun guo zhi (0.44 g/kg),pH(3.73he 3.54)xian zhe di yu bu jie jun guo zhi (4.68);li zhi nei sheng chang mo ming chuan zhu jun fa jiao guo zhi zhong de wei sheng su (Vc)han liang gao yu zhi wu ru gan jun he bu jie jun guo zhi ,dui guo zhi zhong guo tang he pu tao tang de li yong neng li ye jiang yu zhi wu ru gan jun ,liang zhu jun fa jiao guo zhi de pu tao tang han liang fen bie jiao bu jie jun guo zhi xia jiang 31.4%he 26.7%,guo tang han liang jiang fu fen bie wei 43.4%he 21.7%;li zhi nei sheng chang mo ming chuan zhu jun fa jiao guo zhi zhong you li an ji suan bao liu lv wei 87.3%,lve gao yu zhi wu ru gan jun (84.4%),liang zhu jun zai fa jiao guo cheng zhong ge chong an ji suan de chong lei he han liang bian hua you suo bu tong 。【jie lun 】shai shua de li zhi nei sheng chang mo ming chuan zhu jun zai li zhi guo zhi zhong sheng chang liang hao ,fa jiao de li zhi guo zhi ji bao cun he you hua guo zhi de ying yang he kou gan ,you zeng tian yi sheng jun de bao jian gong neng 。
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论文作者分别是来自南方农业学报的胡珊,黄皓,梁卫驱,罗华建,陈仕丽,徐匆,钟鸣，发表于刊物南方农业学报2019年01期论文，是一篇关于荔枝论文,内生菌论文,筛选论文,鉴定论文,肠膜明串珠菌葡聚糖亚种论文,发酵果汁论文,南方农业学报2019年01期论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自南方农业学报2019年01期论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：荔枝论文; 内生菌论文; 筛选论文; 鉴定论文; 肠膜明串珠菌葡聚糖亚种论文; 发酵果汁论文; 南方农业学报2019年01期论文;