本文主要研究内容
作者肖斌（2019）在《公鼠束缚应激诱发精子和生精细胞凋亡：Fas/FasL系统通过促进附睾TNF-α和TRAIL分泌引起精子凋亡》一文中研究指出：现代社会充满了压力,压力是一种严重的心理应激,心理应激会造成不孕不育。因此人们的生殖健康正面临巨大的挑战。应激对雄性生殖能力的损伤可能通过破坏生殖内分泌和造成细胞凋亡等方式进行,破坏精子发生过程,损伤精子质量,进而降低精液质量,同时关于应激对成熟精子影响的研究较少。在很大程度上,心理应激损伤精液质量的机制尚不明确。因为束缚应激模型可以较好地模拟人类日常生活压力所造成的心理应激。所以本研究中我们用小鼠束缚应激模型模拟心理应激研究了束缚应激成年公鼠导致各类生精细胞和精子凋亡的情况及其与Fas/FasL系统的关系。我们对昆明白公鼠束缚应激48小时后,检测其精子的活力、精子和生精细胞的凋亡情况及精子和生精细胞内活化Caspase-3和Fas蛋白的表达情况、附睾尾中活化Caspase-3和Fas/FasL蛋白的表达情况,同时用FasL功能缺失型突变的gld品系小鼠和gld的对照鼠—野生型C57BL/6J品系小鼠进行相关实验,并在体外添加sFasL保存精子后检测精子活力和凋亡,证实了Fas/FasL信号系统在束缚应激中对精子和生精细胞的促凋亡作用,阐明了该系统在束缚应激过程中促进附睾尾中成熟精子凋亡的机制。实验结果如下:(1)束缚应激导致公鼠血清皮质醇水平显著升高,在束缚应激开始后1小时、24小时和48小时,小鼠皮质醇水平始终显著高于对照组,说明我们的应激模型可以有效造成公鼠应激;(2)束缚应激组与对照组精子密度没有显著差异;(3)束缚应激组与对照组新取出的精子活力没有显著差异,但自体外保存90分钟起,束缚组精子活力显著低于对照组,说明束缚应激加快了体外条件下精子活力下降的速度;(4)束缚应激导致Annexin-V FITC阳性精子比例显著升高,精子中活化Caspase-3和Fas蛋白含量显著升高,说明束缚应激48小时促进精子凋亡,且束缚应激对精子的促凋亡作用可能与Fas/FasL系统有关;(5)束缚应激48小时导致附睾尾活化Caspase-3和Fas/FasL蛋白表达水平显著升高,说明束缚应激会促进附睾凋亡;(6)束缚应激48小时导致Annexin阳性生精细胞比例显著升高,生精细胞中活化Caspase-3蛋白表达量显著升高,说明束缚应激48小时促进生精细胞凋亡;束缚应激后第35天束缚组精子活力显著低于对照组,凋亡比例显著高于对照组,说明束缚应激导致生精细胞凋亡进而影响其分化而来的精子的质量;(7)对照组gld和C57BL/6J小鼠精子的体外受精率、凋亡比例,生精细胞凋亡比例均无显著差异,束缚应激48小时后,gld和C57BL/6J小鼠上述指标均发生显著不良变化,但gld小鼠各指标受影响的程度显著小于C57BL/6J小鼠,这说明Fas/FasL系统参与束缚应激导致的精子和生精细胞质量损伤,但也可能存在其他通路在这一过程中发挥作用;(8)精子体外保存过程中添加sFasL并不影响精子活力和凋亡,而sTNF-α显著降低精子活力并升高其凋亡比例,对照组gld和C57BL/6J小鼠附睾尾TNF-α和TRAIL表达量无显著差异,束缚应激48小时后,附睾尾中FasL蛋白表达显著上升,gld和C57BL/6J小鼠附睾尾TNF-α和TRAIL表达量均显著上升,但gld小鼠TNF-α和TRAIL表达量均显著低于C57BL/6J小鼠,这说明束缚应激上调附睾尾中FasL表达促进TNF-α和TRAIL分泌间接导致精子凋亡,但也可能存在独立于Fas/FasL系统之外的通路(TNF-α/TNFR1或TRAIL/TRAILR)在其中发挥作用,且作用强于Fas/FasL系统。因此,我们的结果表明:束缚应激模型能有效造成公鼠应激,束缚应激通过激活Fas/FasL系统促进成熟精子凋亡,并通过导致生精细胞凋亡产生低质量精子,在附睾中FasL因束缚应激而表达升高,促进TNF-α和TRAIL的产生,间接导致精子凋亡。
Abstract
xian dai she hui chong man le ya li ,ya li shi yi chong yan chong de xin li ying ji ,xin li ying ji hui zao cheng bu yun bu yo 。yin ci ren men de sheng shi jian kang zheng mian lin ju da de tiao zhan 。ying ji dui xiong xing sheng shi neng li de sun shang ke neng tong guo po huai sheng shi nei fen bi he zao cheng xi bao diao wang deng fang shi jin hang ,po huai jing zi fa sheng guo cheng ,sun shang jing zi zhi liang ,jin er jiang di jing ye zhi liang ,tong shi guan yu ying ji dui cheng shou jing zi ying xiang de yan jiu jiao shao 。zai hen da cheng du shang ,xin li ying ji sun shang jing ye zhi liang de ji zhi shang bu ming que 。yin wei shu fu ying ji mo xing ke yi jiao hao de mo ni ren lei ri chang sheng huo ya li suo zao cheng de xin li ying ji 。suo yi ben yan jiu zhong wo men yong xiao shu shu fu ying ji mo xing mo ni xin li ying ji yan jiu le shu fu ying ji cheng nian gong shu dao zhi ge lei sheng jing xi bao he jing zi diao wang de qing kuang ji ji yu Fas/FasLji tong de guan ji 。wo men dui kun ming bai gong shu shu fu ying ji 48xiao shi hou ,jian ce ji jing zi de huo li 、jing zi he sheng jing xi bao de diao wang qing kuang ji jing zi he sheng jing xi bao nei huo hua Caspase-3he Fasdan bai de biao da qing kuang 、fu gao wei zhong huo hua Caspase-3he Fas/FasLdan bai de biao da qing kuang ,tong shi yong FasLgong neng que shi xing tu bian de gldpin ji xiao shu he gldde dui zhao shu —ye sheng xing C57BL/6Jpin ji xiao shu jin hang xiang guan shi yan ,bing zai ti wai tian jia sFasLbao cun jing zi hou jian ce jing zi huo li he diao wang ,zheng shi le Fas/FasLxin hao ji tong zai shu fu ying ji zhong dui jing zi he sheng jing xi bao de cu diao wang zuo yong ,chan ming le gai ji tong zai shu fu ying ji guo cheng zhong cu jin fu gao wei zhong cheng shou jing zi diao wang de ji zhi 。shi yan jie guo ru xia :(1)shu fu ying ji dao zhi gong shu xie qing pi zhi chun shui ping xian zhe sheng gao ,zai shu fu ying ji kai shi hou 1xiao shi 、24xiao shi he 48xiao shi ,xiao shu pi zhi chun shui ping shi zhong xian zhe gao yu dui zhao zu ,shui ming wo men de ying ji mo xing ke yi you xiao zao cheng gong shu ying ji ;(2)shu fu ying ji zu yu dui zhao zu jing zi mi du mei you xian zhe cha yi ;(3)shu fu ying ji zu yu dui zhao zu xin qu chu de jing zi huo li mei you xian zhe cha yi ,dan zi ti wai bao cun 90fen zhong qi ,shu fu zu jing zi huo li xian zhe di yu dui zhao zu ,shui ming shu fu ying ji jia kuai le ti wai tiao jian xia jing zi huo li xia jiang de su du ;(4)shu fu ying ji dao zhi Annexin-V FITCyang xing jing zi bi li xian zhe sheng gao ,jing zi zhong huo hua Caspase-3he Fasdan bai han liang xian zhe sheng gao ,shui ming shu fu ying ji 48xiao shi cu jin jing zi diao wang ,ju shu fu ying ji dui jing zi de cu diao wang zuo yong ke neng yu Fas/FasLji tong you guan ;(5)shu fu ying ji 48xiao shi dao zhi fu gao wei huo hua Caspase-3he Fas/FasLdan bai biao da shui ping xian zhe sheng gao ,shui ming shu fu ying ji hui cu jin fu gao diao wang ;(6)shu fu ying ji 48xiao shi dao zhi Annexinyang xing sheng jing xi bao bi li xian zhe sheng gao ,sheng jing xi bao zhong huo hua Caspase-3dan bai biao da liang xian zhe sheng gao ,shui ming shu fu ying ji 48xiao shi cu jin sheng jing xi bao diao wang ;shu fu ying ji hou di 35tian shu fu zu jing zi huo li xian zhe di yu dui zhao zu ,diao wang bi li xian zhe gao yu dui zhao zu ,shui ming shu fu ying ji dao zhi sheng jing xi bao diao wang jin er ying xiang ji fen hua er lai de jing zi de zhi liang ;(7)dui zhao zu gldhe C57BL/6Jxiao shu jing zi de ti wai shou jing lv 、diao wang bi li ,sheng jing xi bao diao wang bi li jun mo xian zhe cha yi ,shu fu ying ji 48xiao shi hou ,gldhe C57BL/6Jxiao shu shang shu zhi biao jun fa sheng xian zhe bu liang bian hua ,dan gldxiao shu ge zhi biao shou ying xiang de cheng du xian zhe xiao yu C57BL/6Jxiao shu ,zhe shui ming Fas/FasLji tong can yu shu fu ying ji dao zhi de jing zi he sheng jing xi bao zhi liang sun shang ,dan ye ke neng cun zai ji ta tong lu zai zhe yi guo cheng zhong fa hui zuo yong ;(8)jing zi ti wai bao cun guo cheng zhong tian jia sFasLbing bu ying xiang jing zi huo li he diao wang ,er sTNF-αxian zhe jiang di jing zi huo li bing sheng gao ji diao wang bi li ,dui zhao zu gldhe C57BL/6Jxiao shu fu gao wei TNF-αhe TRAILbiao da liang mo xian zhe cha yi ,shu fu ying ji 48xiao shi hou ,fu gao wei zhong FasLdan bai biao da xian zhe shang sheng ,gldhe C57BL/6Jxiao shu fu gao wei TNF-αhe TRAILbiao da liang jun xian zhe shang sheng ,dan gldxiao shu TNF-αhe TRAILbiao da liang jun xian zhe di yu C57BL/6Jxiao shu ,zhe shui ming shu fu ying ji shang diao fu gao wei zhong FasLbiao da cu jin TNF-αhe TRAILfen bi jian jie dao zhi jing zi diao wang ,dan ye ke neng cun zai du li yu Fas/FasLji tong zhi wai de tong lu (TNF-α/TNFR1huo TRAIL/TRAILR)zai ji zhong fa hui zuo yong ,ju zuo yong jiang yu Fas/FasLji tong 。yin ci ,wo men de jie guo biao ming :shu fu ying ji mo xing neng you xiao zao cheng gong shu ying ji ,shu fu ying ji tong guo ji huo Fas/FasLji tong cu jin cheng shou jing zi diao wang ,bing tong guo dao zhi sheng jing xi bao diao wang chan sheng di zhi liang jing zi ,zai fu gao zhong FasLyin shu fu ying ji er biao da sheng gao ,cu jin TNF-αhe TRAILde chan sheng ,jian jie dao zhi jing zi diao wang 。
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论文作者分别是来自山东农业大学的肖斌，发表于刊物山东农业大学2019-07-08论文，是一篇关于凋亡论文,束缚应激论文,精子发生论文,精液质量论文,山东农业大学2019-07-08论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自山东农业大学2019-07-08论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：凋亡论文; 束缚应激论文; 精子发生论文; 精液质量论文; 山东农业大学2019-07-08论文;