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作者（2019）在《Baculovirus-expressed FAdV-4 penton base protein protects chicken against hepatitis-hydropericardium syndrome》一文中研究指出：Hepatitis-hydropericardium syndrome(HHS) is an infectious disease caused by fowl adenovirus serotype 4(FAdV-4). Several structural and non-structural proteins of FAdV-4 have been expressed in Escherichia coli and baculovirus expression system to develop candidate subunit vaccines. However, the protective efficiency of baculovirus-expressed penton base protein has not been assessed. In this study, two recombinant capsid proteins, penton base and fiber-2, were constructed. And then, penton base and fiber-2 were administrated alone or together to specific pathogen-free(SPF) chickens at 14 days of life and boosted at 28 days of life. At 42 days of life, the immunized groups and the control group were challenged with FAdV-4 virulent strain. Results show that inoculating penton base or penton base+fiber-2 provided 100% protection to the chickens. All groups vaccinated with the recombinant protein produced detectable antibodies and showed no apparent lesions. Thus, baculovirus-expressed penton base protein is a promising candidate subunit vaccine.
Abstract
Hepatitis-hydropericardium syndrome(HHS) is an infectious disease caused by fowl adenovirus serotype 4(FAdV-4). Several structural and non-structural proteins of FAdV-4 have been expressed in Escherichia coli and baculovirus expression system to develop candidate subunit vaccines. However, the protective efficiency of baculovirus-expressed penton base protein has not been assessed. In this study, two recombinant capsid proteins, penton base and fiber-2, were constructed. And then, penton base and fiber-2 were administrated alone or together to specific pathogen-free(SPF) chickens at 14 days of life and boosted at 28 days of life. At 42 days of life, the immunized groups and the control group were challenged with FAdV-4 virulent strain. Results show that inoculating penton base or penton base+fiber-2 provided 100% protection to the chickens. All groups vaccinated with the recombinant protein produced detectable antibodies and showed no apparent lesions. Thus, baculovirus-expressed penton base protein is a promising candidate subunit vaccine.
论文参考文献
[1].Novel potentiometric application for the determination of amprolium HCl in its single and combined dosage form and in chicken liver[J]. Mai A. Basha,Mohamed K. Abd El-Rahman,Lories I. Bebawy,Maissa Y. Salem. Chinese Chemical Letters.2017(03)[2].The genetic regulation of skeletal muscle development:insights from chicken studies[J]. Wen LUO,Bahareldin A.ABDALLA,Qinghua NIE,Xiquan ZHANG. Frontiers of Agricultural Science and Engineering.2017(03)[3].Cloning Sequencing and Bioinformatics Analysis of GRX2 and TRX2 from Puerpiao chicken and Tengchongxue chicken[J]. Fang Fang,Zhang Chunyong,Li Yanxuan,Li Meiquan,An Qingcong,Chen Kelin,Guo Rongfu. Animal Husbandry and Feed Science.2014(03)[4].Effect of the C.–1388 A>G polymorphism in chicken heat shock transcription factor 3 gene on heat tolerance[J]. ZHANG Wen-wu,KONG Li-na,ZHANG De-xiang,JI Cong-liang,ZHANG Xi-quan,LUO Qing-bin. Journal of Integrative Agriculture.2015(09)[5].Single nucleotide polymorphisms in chicken lmbr1 gene were associated with chicken growth and carcass traits[J]. HUANG YanQun1,2,3, DU XiaoHui2, DENG XueMei4, QIU XiangPin2, WANG ChunKao4, CHEN Wen3, LI Ning1 & WU ChangXin4 1 State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing 100094, China; 2 College of Animal Science, Sichuan Agricultural University, Ya’an 625014, China; 3 College of Animal Husbandry and Veterinary, Henan Agricultural University, Zhengzhou 450002, China; 4 College of Animal Science and Technology, China Agricultural University, Beijing 100094, China. Science in China(Series C:Life Sciences).2007(01)[6].优质鸡育种和生产中若干问题的讨论[J]. 张细权. 中国禽业导刊.2000(12)[7].Ontogenetic expression of chicken growth hormone receptor gene[J]. 李宁,陈永福. Progress in Natural Science.1995(06)[8].Hypoxia promotes cell proliferation by modulating E2F1 in chicken pulmonary arterial smooth muscle cells[J]. Ying Yang,Feng Sun,Chen Zhang,Hao Wang,Guoyao Wu,Zhenlong Wu. Journal of Animal Science and Biotechnology.2013(03)[9].Insulin-like growth factor 2 as a candidate gene influencing growth and carcass traits and its bialleleic expression in chicken[J]. WANG Genyu1,YAN Bingxue1,DENG Xuemei2,LI Changlü2,HU Xiaoxiang1 & Li Ning1 1.State Key Laboratory for Agrobiotechnology,China Agricultural University,Beijing 100094,China;2.Department of Animal Genetics and Breeding,College of Animal Science and Technology,China Agricultural University,Beijing 100094,China. Science in China(Series C:Life Sciences).2005(02)[10].Two new lncRNAs regulate the key immune factor NOD1 and TRAF5 in chicken lymphocyte[J]. BI Yu-lin,YUAN Xiao-ya,CHEN Ying,CHANG Guo-bin,CHEN Guo-hong. Journal of Integrative Agriculture.2019(11)
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：Baculovirus-expressed FAdV-4 penton base protein protects chicken against hepatitis-hydropericardium syndrome论文
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