本文主要研究内容
作者乔进平（2019）在《翅果油对高脂饮食诱导的非酒精性脂肪肝小鼠的改善作用》一文中研究指出：翅果油是翅果油树种仁中提取的油脂,富含不饱和脂肪酸,含量达到90%以上,其中主要是亚油酸、油酸和亚麻酸,具有很高的营养价值。本论文以翅果油作为主要原料,以C57BL/6小鼠为研究对象,探讨翅果油对高脂饮食诱导的非酒精性脂肪肝小鼠的改善作用并对干预机制做初步研究。具体内容及结果如下:(1)4周龄C57BL/6小鼠适应性喂养1周后,将40只小鼠随机分为5组(n=8):基础饮食组、高脂饮食组、翅果油低、中、高剂量组,灌胃干预8周。实验期间,记录小鼠体重和摄食量;实验结束后测定体长、脂肪重、血脂(TC、TG、HDL-C和LDL-C)水平等基础指标。结果表明:与基础饮食组相比,高脂饮食组体重、脂肪重、Lee’s指数及血脂水平显著升高(P<0.05),说明高脂饮食极易导致肥胖和高血脂;翅果油干预后显著降低小鼠体重、脂肪重、Lee’s指数及血清TC、TG、LDL-C水平(P<0.05),其中翅果油中剂量组干预效果最好;且H&E染色结果显示,翅果油干预能够抑制高脂饮食引起的脂肪细胞增大,说明翅果油对高脂饮食引起肥胖、高血脂等的发生具有显著抑制作用。(2)动物实验结束后称量肝脏重量,H&E染色观察肝脏组织病理学切片、测定肝脂(TG,TC),肝损伤(AST,ALT)和氧化应激(SOD,GSH,MDA)指标。结果表明:与基础饮食组相比,高脂饮食组小鼠肝重、血清ALT和AST水平、肝脏TG、TC水平、肝脏MDA水平显著升高(P<0.05),肝脏SOD及GSH水平显著降低(P<0.05),且肝脏组织病理学切片显示,高脂饮食组小鼠肝索排列混乱且出现大面积小泡型脂肪变性,说明高脂饮食可诱导非酒精性脂肪肝疾病产生;翅果油干预后,翅果油干预后各项指标均有改善,说明翅果油对非酒精性脂肪肝具有改善作用;相关性结果表明,体重及血清TG与非酒精性脂肪肝相关因子具有显著相关性,说明翅果油可以通过预防肥胖及高血脂改善非酒精性脂肪肝。(3)肝脏脂质合成代谢紊乱是形成非酒精性脂肪肝的主要因素之一。因此,采用实时荧光定量PCR技术,对肝脏脂质合成相关因子(SREBP-1c,PPARγ,ACC和FAS基因)和脂质代谢相关因子(PPARα,CPT-1和ACOX基因)的mRNA表达进行分析,进一步研究翅果油对高脂饮食诱导的非酒精性脂肪肝小鼠改善作用的作用机制。结果表明:翅果油干预使脏脏SREBP-1c,PPARγ和FAS基因mRNA表达下调,使肝脏PPARα和CPT-1基因mRNA表达上调,说明翅果油可通过抑制肝脏脂质合成和加速脂质分解来改善高脂饮食诱导的非酒精性脂肪肝。(4)采用气相色谱/质谱联用法(GC/MS)对肝脏脂肪酸成分及含量进行分析,结果共检测出C14:0,C16:0,C16:1,C16:2,C17:0,C18:0,C18:1,C18:2,ɑ-C18:3,?-C18:3,C20:1,C20:2,C20:3,C20:4和C22:6 15种脂肪酸,OPLS-DA分析结果表明:与高脂饮食组相比,翅果油干预后肝脏C16:2,C18:1,C18:2,ɑ-C18:3,?-C18:3,C20:4和C22:6这7种脂肪酸差异较大;不饱和度分析结果表明,翅果油干预组与高脂饮食组相比,多不饱和脂肪酸尤其是n-3多不饱和脂肪酸差异较大,说明翅果油干预主要影响n-3多不饱和脂肪酸参与肝脏脂肪酸代谢从而改善非酒精性脂肪肝。
Abstract
chi guo you shi chi guo you shu chong ren zhong di qu de you zhi ,fu han bu bao he zhi fang suan ,han liang da dao 90%yi shang ,ji zhong zhu yao shi ya you suan 、you suan he ya ma suan ,ju you hen gao de ying yang jia zhi 。ben lun wen yi chi guo you zuo wei zhu yao yuan liao ,yi C57BL/6xiao shu wei yan jiu dui xiang ,tan tao chi guo you dui gao zhi yin shi you dao de fei jiu jing xing zhi fang gan xiao shu de gai shan zuo yong bing dui gan yu ji zhi zuo chu bu yan jiu 。ju ti nei rong ji jie guo ru xia :(1)4zhou ling C57BL/6xiao shu kuo ying xing wei yang 1zhou hou ,jiang 40zhi xiao shu sui ji fen wei 5zu (n=8):ji chu yin shi zu 、gao zhi yin shi zu 、chi guo you di 、zhong 、gao ji liang zu ,guan wei gan yu 8zhou 。shi yan ji jian ,ji lu xiao shu ti chong he she shi liang ;shi yan jie shu hou ce ding ti chang 、zhi fang chong 、xie zhi (TC、TG、HDL-Che LDL-C)shui ping deng ji chu zhi biao 。jie guo biao ming :yu ji chu yin shi zu xiang bi ,gao zhi yin shi zu ti chong 、zhi fang chong 、Lee’szhi shu ji xie zhi shui ping xian zhe sheng gao (P<0.05),shui ming gao zhi yin shi ji yi dao zhi fei pan he gao xie zhi ;chi guo you gan yu hou xian zhe jiang di xiao shu ti chong 、zhi fang chong 、Lee’szhi shu ji xie qing TC、TG、LDL-Cshui ping (P<0.05),ji zhong chi guo you zhong ji liang zu gan yu xiao guo zui hao ;ju H&Eran se jie guo xian shi ,chi guo you gan yu neng gou yi zhi gao zhi yin shi yin qi de zhi fang xi bao zeng da ,shui ming chi guo you dui gao zhi yin shi yin qi fei pan 、gao xie zhi deng de fa sheng ju you xian zhe yi zhi zuo yong 。(2)dong wu shi yan jie shu hou chen liang gan zang chong liang ,H&Eran se guan cha gan zang zu zhi bing li xue qie pian 、ce ding gan zhi (TG,TC),gan sun shang (AST,ALT)he yang hua ying ji (SOD,GSH,MDA)zhi biao 。jie guo biao ming :yu ji chu yin shi zu xiang bi ,gao zhi yin shi zu xiao shu gan chong 、xie qing ALThe ASTshui ping 、gan zang TG、TCshui ping 、gan zang MDAshui ping xian zhe sheng gao (P<0.05),gan zang SODji GSHshui ping xian zhe jiang di (P<0.05),ju gan zang zu zhi bing li xue qie pian xian shi ,gao zhi yin shi zu xiao shu gan suo pai lie hun luan ju chu xian da mian ji xiao pao xing zhi fang bian xing ,shui ming gao zhi yin shi ke you dao fei jiu jing xing zhi fang gan ji bing chan sheng ;chi guo you gan yu hou ,chi guo you gan yu hou ge xiang zhi biao jun you gai shan ,shui ming chi guo you dui fei jiu jing xing zhi fang gan ju you gai shan zuo yong ;xiang guan xing jie guo biao ming ,ti chong ji xie qing TGyu fei jiu jing xing zhi fang gan xiang guan yin zi ju you xian zhe xiang guan xing ,shui ming chi guo you ke yi tong guo yu fang fei pan ji gao xie zhi gai shan fei jiu jing xing zhi fang gan 。(3)gan zang zhi zhi ge cheng dai xie wen luan shi xing cheng fei jiu jing xing zhi fang gan de zhu yao yin su zhi yi 。yin ci ,cai yong shi shi ying guang ding liang PCRji shu ,dui gan zang zhi zhi ge cheng xiang guan yin zi (SREBP-1c,PPARγ,ACChe FASji yin )he zhi zhi dai xie xiang guan yin zi (PPARα,CPT-1he ACOXji yin )de mRNAbiao da jin hang fen xi ,jin yi bu yan jiu chi guo you dui gao zhi yin shi you dao de fei jiu jing xing zhi fang gan xiao shu gai shan zuo yong de zuo yong ji zhi 。jie guo biao ming :chi guo you gan yu shi zang zang SREBP-1c,PPARγhe FASji yin mRNAbiao da xia diao ,shi gan zang PPARαhe CPT-1ji yin mRNAbiao da shang diao ,shui ming chi guo you ke tong guo yi zhi gan zang zhi zhi ge cheng he jia su zhi zhi fen jie lai gai shan gao zhi yin shi you dao de fei jiu jing xing zhi fang gan 。(4)cai yong qi xiang se pu /zhi pu lian yong fa (GC/MS)dui gan zang zhi fang suan cheng fen ji han liang jin hang fen xi ,jie guo gong jian ce chu C14:0,C16:0,C16:1,C16:2,C17:0,C18:0,C18:1,C18:2,兺 -C18:3,?-C18:3,C20:1,C20:2,C20:3,C20:4he C22:6 15chong zhi fang suan ,OPLS-DAfen xi jie guo biao ming :yu gao zhi yin shi zu xiang bi ,chi guo you gan yu hou gan zang C16:2,C18:1,C18:2,兺 -C18:3,?-C18:3,C20:4he C22:6zhe 7chong zhi fang suan cha yi jiao da ;bu bao he du fen xi jie guo biao ming ,chi guo you gan yu zu yu gao zhi yin shi zu xiang bi ,duo bu bao he zhi fang suan you ji shi n-3duo bu bao he zhi fang suan cha yi jiao da ,shui ming chi guo you gan yu zhu yao ying xiang n-3duo bu bao he zhi fang suan can yu gan zang zhi fang suan dai xie cong er gai shan fei jiu jing xing zhi fang gan 。
论文参考文献
论文详细介绍
论文作者分别是来自山西大学的乔进平，发表于刊物山西大学2019-11-12论文，是一篇关于翅果油论文,非酒精性脂肪肝论文,高脂饮食论文,脂质代谢论文,脂肪酸论文,山西大学2019-11-12论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自山西大学2019-11-12论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：翅果油论文; 非酒精性脂肪肝论文; 高脂饮食论文; 脂质代谢论文; 脂肪酸论文; 山西大学2019-11-12论文;