本文主要研究内容
作者王洁丽（2019）在《驯化对大豆根系微生物群落组装和根瘤内生菌多样性影响的研究》一文中研究指出：植物驯化和育种是农业产生和发展的基础,对人类社会文明产生巨大影响。现代品种的狭窄遗传背景正逐渐成为作物改良的主要瓶颈,而近缘野生种类为作物的驯化和育种提供了机遇。根际微生物已被证明可赋予植物新的抗逆性与生态功能。然而,植物驯化是否影响根际微生物群落的组装和生态功能还不清楚。本文以野生、半野生、栽培和广适种大豆根系微域为研究对象,通过盆栽试验,分别将这些大豆品种种植于来自东北、黄淮海和南方地区的土壤中,再通过16S rDNA高通量测序技术,研究不同大豆根区、根际、根内、根瘤内等四个部位微生物群落的结构与组成,分析不同大豆品种之间根际微生物群落的差异,探究了不同大豆品种对根瘤内生菌群落的影响。得到以下结果:(1)大豆根系微生物群落主要受土壤、根系部位和大豆品种的影响。东北、黄淮海和南方地区的大豆根系微生物群落具有显著差异。从根外到根内,微生物的丰富度和多样性逐渐降低,差异OTUs逐渐增多,根系的过滤作用逐渐增强,且不同大豆品种根系微域对OTUs具有各自的选择过滤作用。通过网络分析发现,野生大豆根内细菌网络结构更复杂,半野生、栽培和广适种大豆根际细菌网络更复杂,根内细菌群落易受大豆种类影响,并且根区、根际和根内的关键物种不同。(2)不同土壤中大豆根际微生物群落结构具有显著差异。从野生、半野生到栽培再到广适种大豆,在东北土壤中,根际细菌丰富度和多样性沿此顺序逐渐降低,然而在黄淮海和南方土壤中,丰富度和多样性变化与之相反;不同大豆品种的核心OTUs数目所占比例较小,但其相对丰度较大。不同大豆品种共同拥有的核心类群,包括Ensifer、Bradyrhizobium、Mesorhizobium、Rhizobium、Pseudomonas、Novosphingobium、Sphingomonas、Massilia、Piscinibacter、Pseudoduganella、Ramlibacter、Variovorax和2个未分类的OTUs。大豆生物表型与不同大豆品种的根际微生物群落显著相关,在OTU水平上,植株全长(株高与根长之和)与野生大豆根际微生物丰度显著正相关,鲜重与栽培大豆根际微生物丰度显著负相关。(3)不同大豆品种之间的根瘤内生菌群落差异显著,且根瘤内生菌群落受土壤类型影响大,其次是不同大豆品种。根瘤内生菌沿着野生、半野生、栽培和广适种大豆的顺序,丰富度和多样性呈逐渐增高。根据根瘤功能特点,我们又将根瘤内生菌分为共生根瘤菌群落和非共生菌群落,结果发现在共生根瘤菌群落中Bradyrhizobium在东北和南方土壤中占主导地位,Ensifer在黄淮海地区占主导地位。在非共生内生菌群落中,慢生根瘤菌科、丛毛单胞菌科、鞘酯菌科、根瘤菌科和肠杆菌科等为主要类群。本研究揭示了野生、半野生、栽培和广适种大豆根系微生物群落组装规律及影响其根瘤内生菌群落多样性的因素,为大豆有益菌群的恢复和利用奠定基础,也为未来育种提供新的思路。
Abstract
zhi wu xun hua he yo chong shi nong ye chan sheng he fa zhan de ji chu ,dui ren lei she hui wen ming chan sheng ju da ying xiang 。xian dai pin chong de xia zhai wei chuan bei jing zheng zhu jian cheng wei zuo wu gai liang de zhu yao ping geng ,er jin yuan ye sheng chong lei wei zuo wu de xun hua he yo chong di gong le ji yu 。gen ji wei sheng wu yi bei zheng ming ke fu yu zhi wu xin de kang ni xing yu sheng tai gong neng 。ran er ,zhi wu xun hua shi fou ying xiang gen ji wei sheng wu qun la de zu zhuang he sheng tai gong neng hai bu qing chu 。ben wen yi ye sheng 、ban ye sheng 、zai pei he an kuo chong da dou gen ji wei yu wei yan jiu dui xiang ,tong guo pen zai shi yan ,fen bie jiang zhe xie da dou pin chong chong zhi yu lai zi dong bei 、huang huai hai he na fang de ou de tu rang zhong ,zai tong guo 16S rDNAgao tong liang ce xu ji shu ,yan jiu bu tong da dou gen ou 、gen ji 、gen nei 、gen liu nei deng si ge bu wei wei sheng wu qun la de jie gou yu zu cheng ,fen xi bu tong da dou pin chong zhi jian gen ji wei sheng wu qun la de cha yi ,tan jiu le bu tong da dou pin chong dui gen liu nei sheng jun qun la de ying xiang 。de dao yi xia jie guo :(1)da dou gen ji wei sheng wu qun la zhu yao shou tu rang 、gen ji bu wei he da dou pin chong de ying xiang 。dong bei 、huang huai hai he na fang de ou de da dou gen ji wei sheng wu qun la ju you xian zhe cha yi 。cong gen wai dao gen nei ,wei sheng wu de feng fu du he duo yang xing zhu jian jiang di ,cha yi OTUszhu jian zeng duo ,gen ji de guo lv zuo yong zhu jian zeng jiang ,ju bu tong da dou pin chong gen ji wei yu dui OTUsju you ge zi de shua ze guo lv zuo yong 。tong guo wang lao fen xi fa xian ,ye sheng da dou gen nei xi jun wang lao jie gou geng fu za ,ban ye sheng 、zai pei he an kuo chong da dou gen ji xi jun wang lao geng fu za ,gen nei xi jun qun la yi shou da dou chong lei ying xiang ,bing ju gen ou 、gen ji he gen nei de guan jian wu chong bu tong 。(2)bu tong tu rang zhong da dou gen ji wei sheng wu qun la jie gou ju you xian zhe cha yi 。cong ye sheng 、ban ye sheng dao zai pei zai dao an kuo chong da dou ,zai dong bei tu rang zhong ,gen ji xi jun feng fu du he duo yang xing yan ci shun xu zhu jian jiang di ,ran er zai huang huai hai he na fang tu rang zhong ,feng fu du he duo yang xing bian hua yu zhi xiang fan ;bu tong da dou pin chong de he xin OTUsshu mu suo zhan bi li jiao xiao ,dan ji xiang dui feng du jiao da 。bu tong da dou pin chong gong tong yong you de he xin lei qun ,bao gua Ensifer、Bradyrhizobium、Mesorhizobium、Rhizobium、Pseudomonas、Novosphingobium、Sphingomonas、Massilia、Piscinibacter、Pseudoduganella、Ramlibacter、Variovoraxhe 2ge wei fen lei de OTUs。da dou sheng wu biao xing yu bu tong da dou pin chong de gen ji wei sheng wu qun la xian zhe xiang guan ,zai OTUshui ping shang ,zhi zhu quan chang (zhu gao yu gen chang zhi he )yu ye sheng da dou gen ji wei sheng wu feng du xian zhe zheng xiang guan ,xian chong yu zai pei da dou gen ji wei sheng wu feng du xian zhe fu xiang guan 。(3)bu tong da dou pin chong zhi jian de gen liu nei sheng jun qun la cha yi xian zhe ,ju gen liu nei sheng jun qun la shou tu rang lei xing ying xiang da ,ji ci shi bu tong da dou pin chong 。gen liu nei sheng jun yan zhao ye sheng 、ban ye sheng 、zai pei he an kuo chong da dou de shun xu ,feng fu du he duo yang xing cheng zhu jian zeng gao 。gen ju gen liu gong neng te dian ,wo men you jiang gen liu nei sheng jun fen wei gong sheng gen liu jun qun la he fei gong sheng jun qun la ,jie guo fa xian zai gong sheng gen liu jun qun la zhong Bradyrhizobiumzai dong bei he na fang tu rang zhong zhan zhu dao de wei ,Ensiferzai huang huai hai de ou zhan zhu dao de wei 。zai fei gong sheng nei sheng jun qun la zhong ,man sheng gen liu jun ke 、cong mao chan bao jun ke 、qiao zhi jun ke 、gen liu jun ke he chang gan jun ke deng wei zhu yao lei qun 。ben yan jiu jie shi le ye sheng 、ban ye sheng 、zai pei he an kuo chong da dou gen ji wei sheng wu qun la zu zhuang gui lv ji ying xiang ji gen liu nei sheng jun qun la duo yang xing de yin su ,wei da dou you yi jun qun de hui fu he li yong dian ding ji chu ,ye wei wei lai yo chong di gong xin de sai lu 。
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论文详细介绍
论文作者分别是来自西北农林科技大学的王洁丽，发表于刊物西北农林科技大学2019-07-11论文，是一篇关于大豆品种论文,根系微生物论文,根瘤内生菌论文,核心微生物论文,西北农林科技大学2019-07-11论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自西北农林科技大学2019-07-11论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：大豆品种论文; 根系微生物论文; 根瘤内生菌论文; 核心微生物论文; 西北农林科技大学2019-07-11论文;