本文主要研究内容
作者张伟（2019）在《海带降解菌的筛选与发酵产物的成分分析及在植物生长中的应用研究》一文中研究指出：随着海洋活性物质研究的不断发展,人们对海藻多糖的关注日益加强。天然海藻酸不仅具有很好的生物活性,而且其降解产物——海藻寡糖同样具有较强的生物活性,具有很大的开发价值和较为广阔的发展前景。本文主要研究海带降解功能菌的分离、筛选和它们对海带的降解功能,海带发酵产物的成分分析,海带发酵液对油菜种子生长发育的影响及其对土壤微生物菌群的影响。(1)海带降解功能菌的分离、鉴定及筛选本文从山东荣成某海带养殖场采集新鲜的海带,对其携带的菌群经过多次富集,得到相对稳定的具有海带降解效果的菌群。通过菌种的分离、纯化从而得到30株菌。对其进行16S rDNA测序及筛选,从而选择降解效果好的菌株及菌种组合。利用果胶或海藻酸钠为唯一碳源的选择性培养基挑选含有果胶酶、海藻酸裂解酶的菌株;通过测定海带发酵液中海藻酸含量及相应菌种的单菌及复合菌降解海带效果选出降解效果较好的单菌及复合菌。结果表明,菌种1-2及含有1-2和3-10的复合菌降解海带效果均在85%以上,其中1-2和3-10复合菌的降解效果为95%,效果较好。而复合菌的降解除几个效果差的外,其它基本达到70%以上,效果相对好于单菌的。(2)海带发酵液检测和成分分析海藻寡糖的粗提取:通过95%的乙醇溶液以粗提取海藻寡糖,并通过3,5-二硝基水杨酸比色法测定海带发酵液中还原糖和总糖含量以确定海藻寡糖的提取情况。由实验可知,1-2和3-10复合菌发酵20%海带的海带发酵液在提取前的还原糖和总糖含量分别为8.76、25.59g/ml,结果最好,经过海藻寡糖的粗提取,海带发酵液中都几乎不含还原糖和总糖,因此通过上述方法可提取出海藻寡糖。薄层层析:实验通过对展开剂和显色剂的分析可知,在展开剂正丁醇:乙酸乙酯:异丙醇:冰乙酸:水=2:6:6:4:1,显色剂使用20%硫酸-甲醇时条带效果比较好。海带发酵液的条带大致在蔗糖附近略靠下。由此可以推测出此海带发酵液中的海藻寡糖应大多为三糖。(3)海带发酵液对油菜种子萌发和生长的影响通过以稀释50、100、500、1000、2000倍海带稀释液,水为对照,处理油菜种子,观察油菜种子的发芽及发芽后根、茎的生长情况。实验表明,海带发酵液稀释1000倍处理的油菜种子发芽率最高为94.5%,比对照组高了58%;海带发酵液稀释2000倍处理的油菜芽根生长情况最好为4.44cm,比对照组高1.23cm;海带发酵液稀释2000倍处理油菜芽生长最好为5.44cm,比对照组高3.52cm。将育苗好的油菜种子种植于盆中,分别浇灌上水稀释倍数的海带稀释液,以水为对照,看油菜的生长状况。实验表明,海带发酵液稀释2000倍灌溉的油菜苗的茎长为7.90cm,比对照组高2.54cm,长于其他稀释液的种子。综上述结果可知,海带发酵液稀释2000倍的对油菜种子的促生作用最好,其次为1000倍的处理组。(4)海藻发酵液对土壤微生物菌群及植物生长的影响将育苗好的油菜种子种植于装有接种土壤菌悬液的无菌土的盆中,浇灌不同的海带稀释液,以水为对照,看油菜的生长状况及土壤微生物的生长状况。实验表明,油菜植株在1500稀释度生长比较好,植物植株大一些;土壤中放线菌、细菌和真菌均为海带发酵液稀释1500倍时的菌数最多分别为2.34×10~7,5.7×10~6,3.14×10~7个,说明此稀释度对土壤微生物的生长促进作用比较明显。
Abstract
sui zhao hai xiang huo xing wu zhi yan jiu de bu duan fa zhan ,ren men dui hai zao duo tang de guan zhu ri yi jia jiang 。tian ran hai zao suan bu jin ju you hen hao de sheng wu huo xing ,er ju ji jiang jie chan wu ——hai zao gua tang tong yang ju you jiao jiang de sheng wu huo xing ,ju you hen da de kai fa jia zhi he jiao wei an kuo de fa zhan qian jing 。ben wen zhu yao yan jiu hai dai jiang jie gong neng jun de fen li 、shai shua he ta men dui hai dai de jiang jie gong neng ,hai dai fa jiao chan wu de cheng fen fen xi ,hai dai fa jiao ye dui you cai chong zi sheng chang fa yo de ying xiang ji ji dui tu rang wei sheng wu jun qun de ying xiang 。(1)hai dai jiang jie gong neng jun de fen li 、jian ding ji shai shua ben wen cong shan dong rong cheng mou hai dai yang shi chang cai ji xin xian de hai dai ,dui ji xie dai de jun qun jing guo duo ci fu ji ,de dao xiang dui wen ding de ju you hai dai jiang jie xiao guo de jun qun 。tong guo jun chong de fen li 、chun hua cong er de dao 30zhu jun 。dui ji jin hang 16S rDNAce xu ji shai shua ,cong er shua ze jiang jie xiao guo hao de jun zhu ji jun chong zu ge 。li yong guo jiao huo hai zao suan na wei wei yi tan yuan de shua ze xing pei yang ji tiao shua han you guo jiao mei 、hai zao suan lie jie mei de jun zhu ;tong guo ce ding hai dai fa jiao ye zhong hai zao suan han liang ji xiang ying jun chong de chan jun ji fu ge jun jiang jie hai dai xiao guo shua chu jiang jie xiao guo jiao hao de chan jun ji fu ge jun 。jie guo biao ming ,jun chong 1-2ji han you 1-2he 3-10de fu ge jun jiang jie hai dai xiao guo jun zai 85%yi shang ,ji zhong 1-2he 3-10fu ge jun de jiang jie xiao guo wei 95%,xiao guo jiao hao 。er fu ge jun de jiang jie chu ji ge xiao guo cha de wai ,ji ta ji ben da dao 70%yi shang ,xiao guo xiang dui hao yu chan jun de 。(2)hai dai fa jiao ye jian ce he cheng fen fen xi hai zao gua tang de cu di qu :tong guo 95%de yi chun rong ye yi cu di qu hai zao gua tang ,bing tong guo 3,5-er xiao ji shui yang suan bi se fa ce ding hai dai fa jiao ye zhong hai yuan tang he zong tang han liang yi que ding hai zao gua tang de di qu qing kuang 。you shi yan ke zhi ,1-2he 3-10fu ge jun fa jiao 20%hai dai de hai dai fa jiao ye zai di qu qian de hai yuan tang he zong tang han liang fen bie wei 8.76、25.59g/ml,jie guo zui hao ,jing guo hai zao gua tang de cu di qu ,hai dai fa jiao ye zhong dou ji hu bu han hai yuan tang he zong tang ,yin ci tong guo shang shu fang fa ke di qu chu hai zao gua tang 。bao ceng ceng xi :shi yan tong guo dui zhan kai ji he xian se ji de fen xi ke zhi ,zai zhan kai ji zheng ding chun :yi suan yi zhi :yi bing chun :bing yi suan :shui =2:6:6:4:1,xian se ji shi yong 20%liu suan -jia chun shi tiao dai xiao guo bi jiao hao 。hai dai fa jiao ye de tiao dai da zhi zai zhe tang fu jin lve kao xia 。you ci ke yi tui ce chu ci hai dai fa jiao ye zhong de hai zao gua tang ying da duo wei san tang 。(3)hai dai fa jiao ye dui you cai chong zi meng fa he sheng chang de ying xiang tong guo yi xi shi 50、100、500、1000、2000bei hai dai xi shi ye ,shui wei dui zhao ,chu li you cai chong zi ,guan cha you cai chong zi de fa ya ji fa ya hou gen 、jing de sheng chang qing kuang 。shi yan biao ming ,hai dai fa jiao ye xi shi 1000bei chu li de you cai chong zi fa ya lv zui gao wei 94.5%,bi dui zhao zu gao le 58%;hai dai fa jiao ye xi shi 2000bei chu li de you cai ya gen sheng chang qing kuang zui hao wei 4.44cm,bi dui zhao zu gao 1.23cm;hai dai fa jiao ye xi shi 2000bei chu li you cai ya sheng chang zui hao wei 5.44cm,bi dui zhao zu gao 3.52cm。jiang yo miao hao de you cai chong zi chong zhi yu pen zhong ,fen bie jiao guan shang shui xi shi bei shu de hai dai xi shi ye ,yi shui wei dui zhao ,kan you cai de sheng chang zhuang kuang 。shi yan biao ming ,hai dai fa jiao ye xi shi 2000bei guan gai de you cai miao de jing chang wei 7.90cm,bi dui zhao zu gao 2.54cm,chang yu ji ta xi shi ye de chong zi 。zeng shang shu jie guo ke zhi ,hai dai fa jiao ye xi shi 2000bei de dui you cai chong zi de cu sheng zuo yong zui hao ,ji ci wei 1000bei de chu li zu 。(4)hai zao fa jiao ye dui tu rang wei sheng wu jun qun ji zhi wu sheng chang de ying xiang jiang yo miao hao de you cai chong zi chong zhi yu zhuang you jie chong tu rang jun xuan ye de mo jun tu de pen zhong ,jiao guan bu tong de hai dai xi shi ye ,yi shui wei dui zhao ,kan you cai de sheng chang zhuang kuang ji tu rang wei sheng wu de sheng chang zhuang kuang 。shi yan biao ming ,you cai zhi zhu zai 1500xi shi du sheng chang bi jiao hao ,zhi wu zhi zhu da yi xie ;tu rang zhong fang xian jun 、xi jun he zhen jun jun wei hai dai fa jiao ye xi shi 1500bei shi de jun shu zui duo fen bie wei 2.34×10~7,5.7×10~6,3.14×10~7ge ,shui ming ci xi shi du dui tu rang wei sheng wu de sheng chang cu jin zuo yong bi jiao ming xian 。
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论文作者分别是来自烟台大学的张伟，发表于刊物烟台大学2019-08-29论文，是一篇关于海带发酵论文,海带降解菌筛选论文,海藻寡糖论文,薄层层析论文,土壤菌群论文,烟台大学2019-08-29论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自烟台大学2019-08-29论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：海带发酵论文; 海带降解菌筛选论文; 海藻寡糖论文; 薄层层析论文; 土壤菌群论文; 烟台大学2019-08-29论文;