本文主要研究内容
作者金丽颖（2019）在《抗黄瓜棒孢叶斑病放线菌的筛选及Streptomyces inhibens多相分类研究》一文中研究指出：黄瓜棒孢叶斑病是由多主棒孢（Corynespora cassiicola）侵染引起的,是近期威胁黄瓜生产的重要流行病害,造成黄瓜的大面积减产,寻找有效的防治措施是促进当前黄瓜种植产业可持续发展的主要任务。目前关于黄瓜棒孢叶斑病的防治方法主要还是采用化学防治,但由于该病原菌易变异,且长期频繁地施加化学试剂如杀菌剂等,又会导致该病原菌产生抗药性,更会引发食品安全和环境问题,因此黄瓜生产特别需要低毒、无害、环境友好型的防治方法。放线菌是一种分布广、种类多、功能性强的微生物,所以近年来常选用放线菌防治植物病害。本研究以黄瓜根际土壤及伴生小麦根际土壤为分离对象,采用多种培养基对放线菌进行分离纯化,通过抗黄瓜棒孢叶斑病原真菌及其他植物病原真菌和抗细菌活性筛选得到一株活性较好的链霉菌Streptomyces NEAU-J2,通过离体叶片和盆栽试验研究了该菌株对黄瓜棒孢叶斑病的防治效果并对其次级代谢产物进行分离,。同时,对得到的另一株活性放线菌新种进行多相分类学研究。本试验主要结果如下:（1）通过五种培养基（GS、HV、AAG、CPA、DPA）共分离出262株放线菌,其中119株分离自黄瓜根际土壤,其余143株分离自伴生小麦根际土壤。（2）通过抗黄瓜棒孢叶斑病病原菌活性初筛和复筛后,有32株放线菌对黄瓜棒孢叶斑病病原菌抑制率达到20%以上,其中有16株抑制率达到了40%以上;选取了抑菌活性最好的10株放线菌进行广谱的抑菌活性测定,发现菌株NEAU-J2具有较好的广谱抑菌作用。（3）黄瓜离体叶片和盆栽试验表明,用NEAU-J2孢子悬液及发酵滤液分别处理黄瓜叶片后,对黄瓜棒孢叶斑病有很好的防治效果。其中发酵滤液对黄瓜棒孢叶斑病的防效最好,在离体叶片和盆栽试验中的防效分别为81.2%和82.1%。（4）通过培养特性分析,菌株NEAU-J2培养特征与链霉菌属吻合,初步确定菌株NEAU-J2属于链霉菌属。此外功能特征分析表明,NEAU-J2发酵液对黄瓜棒孢叶斑病的抑制作用具有良好的紫外线稳定性,且菌株NEAU-J2可以产生几丁质酶,单位菌落透明圈直径为16.2mm。（5）对菌株NEAU-J2的次级代谢产物进行分离,初步得到三个已报道的化合物,分别为17-O-demethyl-geldanamycin、pteridic acid hydrate和pteridic acid F,其中pteridic acid hydrate对黄瓜棒孢叶斑病的抑制作用最好,当pteridic acid hydrate浓度为5 mg?mL-1时,对黄瓜棒孢叶斑病的抑制率为56.3%。（6）本试验通过对黄瓜棒孢叶斑病抑制率最好的10株放线菌测序发现了一株放线菌新种NEAU-D10,采用多相分类学方法分别从分子水平、形态学水平、化学分类水平、生理生化水平及全基因组分析对其进行研究。确定这株放线菌属于链霉菌属,并将其命名为Streptomyces inhibens。
Abstract
huang gua bang bao xie ban bing shi you duo zhu bang bao （Corynespora cassiicola）qin ran yin qi de ,shi jin ji wei xie huang gua sheng chan de chong yao liu hang bing hai ,zao cheng huang gua de da mian ji jian chan ,xun zhao you xiao de fang zhi cuo shi shi cu jin dang qian huang gua chong zhi chan ye ke chi xu fa zhan de zhu yao ren wu 。mu qian guan yu huang gua bang bao xie ban bing de fang zhi fang fa zhu yao hai shi cai yong hua xue fang zhi ,dan you yu gai bing yuan jun yi bian yi ,ju chang ji pin fan de shi jia hua xue shi ji ru sha jun ji deng ,you hui dao zhi gai bing yuan jun chan sheng kang yao xing ,geng hui yin fa shi pin an quan he huan jing wen ti ,yin ci huang gua sheng chan te bie xu yao di du 、mo hai 、huan jing you hao xing de fang zhi fang fa 。fang xian jun shi yi chong fen bu an 、chong lei duo 、gong neng xing jiang de wei sheng wu ,suo yi jin nian lai chang shua yong fang xian jun fang zhi zhi wu bing hai 。ben yan jiu yi huang gua gen ji tu rang ji ban sheng xiao mai gen ji tu rang wei fen li dui xiang ,cai yong duo chong pei yang ji dui fang xian jun jin hang fen li chun hua ,tong guo kang huang gua bang bao xie ban bing yuan zhen jun ji ji ta zhi wu bing yuan zhen jun he kang xi jun huo xing shai shua de dao yi zhu huo xing jiao hao de lian mei jun Streptomyces NEAU-J2,tong guo li ti xie pian he pen zai shi yan yan jiu le gai jun zhu dui huang gua bang bao xie ban bing de fang zhi xiao guo bing dui ji ci ji dai xie chan wu jin hang fen li ,。tong shi ,dui de dao de ling yi zhu huo xing fang xian jun xin chong jin hang duo xiang fen lei xue yan jiu 。ben shi yan zhu yao jie guo ru xia :（1）tong guo wu chong pei yang ji （GS、HV、AAG、CPA、DPA）gong fen li chu 262zhu fang xian jun ,ji zhong 119zhu fen li zi huang gua gen ji tu rang ,ji yu 143zhu fen li zi ban sheng xiao mai gen ji tu rang 。（2）tong guo kang huang gua bang bao xie ban bing bing yuan jun huo xing chu shai he fu shai hou ,you 32zhu fang xian jun dui huang gua bang bao xie ban bing bing yuan jun yi zhi lv da dao 20%yi shang ,ji zhong you 16zhu yi zhi lv da dao le 40%yi shang ;shua qu le yi jun huo xing zui hao de 10zhu fang xian jun jin hang an pu de yi jun huo xing ce ding ,fa xian jun zhu NEAU-J2ju you jiao hao de an pu yi jun zuo yong 。（3）huang gua li ti xie pian he pen zai shi yan biao ming ,yong NEAU-J2bao zi xuan ye ji fa jiao lv ye fen bie chu li huang gua xie pian hou ,dui huang gua bang bao xie ban bing you hen hao de fang zhi xiao guo 。ji zhong fa jiao lv ye dui huang gua bang bao xie ban bing de fang xiao zui hao ,zai li ti xie pian he pen zai shi yan zhong de fang xiao fen bie wei 81.2%he 82.1%。（4）tong guo pei yang te xing fen xi ,jun zhu NEAU-J2pei yang te zheng yu lian mei jun shu wen ge ,chu bu que ding jun zhu NEAU-J2shu yu lian mei jun shu 。ci wai gong neng te zheng fen xi biao ming ,NEAU-J2fa jiao ye dui huang gua bang bao xie ban bing de yi zhi zuo yong ju you liang hao de zi wai xian wen ding xing ,ju jun zhu NEAU-J2ke yi chan sheng ji ding zhi mei ,chan wei jun la tou ming juan zhi jing wei 16.2mm。（5）dui jun zhu NEAU-J2de ci ji dai xie chan wu jin hang fen li ,chu bu de dao san ge yi bao dao de hua ge wu ,fen bie wei 17-O-demethyl-geldanamycin、pteridic acid hydratehe pteridic acid F,ji zhong pteridic acid hydratedui huang gua bang bao xie ban bing de yi zhi zuo yong zui hao ,dang pteridic acid hydratenong du wei 5 mg?mL-1shi ,dui huang gua bang bao xie ban bing de yi zhi lv wei 56.3%。（6）ben shi yan tong guo dui huang gua bang bao xie ban bing yi zhi lv zui hao de 10zhu fang xian jun ce xu fa xian le yi zhu fang xian jun xin chong NEAU-D10,cai yong duo xiang fen lei xue fang fa fen bie cong fen zi shui ping 、xing tai xue shui ping 、hua xue fen lei shui ping 、sheng li sheng hua shui ping ji quan ji yin zu fen xi dui ji jin hang yan jiu 。que ding zhe zhu fang xian jun shu yu lian mei jun shu ,bing jiang ji ming ming wei Streptomyces inhibens。
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论文详细介绍
论文作者分别是来自东北农业大学的金丽颖，发表于刊物东北农业大学2019-08-27论文，是一篇关于黄瓜棒孢叶斑病论文,根际放线菌论文,抑菌活性论文,次级代谢产物论文,多相分类学论文,东北农业大学2019-08-27论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自东北农业大学2019-08-27论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：黄瓜棒孢叶斑病论文; 根际放线菌论文; 抑菌活性论文; 次级代谢产物论文; 多相分类学论文; 东北农业大学2019-08-27论文;