本文主要研究内容
作者张文超（2019）在《碳源及NAD~+前体物调控辅酶Ⅰ对聚磷菌胞内多聚物代谢的影响》一文中研究指出：生物除磷一直是国内水质科学和技术研究的热点问题,调控聚磷菌胞内多聚物代谢是提高生物除磷效率的有效途径。烟酰胺腺嘌呤二核甘酸(辅酶Ⅰ,NADH还原态,NAD~+氧化态)是调节胞内氧化还原反应极其重要的因子,因此,调控胞内辅酶Ⅰ的水平改变聚磷菌胞内多聚物的代谢是提高生物除磷效率的一种新思路。然而,通过调控聚磷菌胞内辅酶Ⅰ水平调节胞内多聚物代谢的研究较少。该研究通过厌氧/好氧交替运行的工艺并结合蓝白斑筛选法筛选一株聚磷菌。针对筛选出的聚磷菌,通过改变碳源和添加不同浓度烟酸的外源调控方法实现辅酶Ⅰ的调节。考察碳源和烟酸对聚磷菌胞内辅酶Ⅰ的调控及对胞内多聚物代谢的影响。主要研究结果如下:(1)通过厌氧/好氧交替运行的工艺快速富集聚磷菌并采用厌氧/好氧交替的平板迭代培养分离聚磷菌,结合蓝白斑筛选和异染颗粒及PHB(聚-β-羟基丁酸)染色,从中筛选出ZWC-1、ZWC-2、ZWC-3、ZWC-4、ZWC-5、ZWC-6、ZWC-7、ZWC-8、ZWC-9、ZWC-10、ZWC-11、ZWC-12、ZWC-13、ZWC14共14株聚磷菌。对14株聚磷菌的除磷性能进行测试,测试结果表明,除磷率分别为44.5%、45.5%、77.5%、71.2%、47.3%、80.4%、76.6%、54.7%、76.2%、71.9%、43.6%、58.6%、75.6%、48.4%。该试验选择ZWC-8菌株作为研究对象。(2)采用革兰氏染色、扫描电镜观察及16S rDNA序列分析和同源性比较对聚磷菌ZWC-8进行鉴定。革兰氏染色结果表明,ZWC-8菌株为革兰氏阴性菌;扫描电镜结果表明,菌株大小为(0.4～0.5)μm×(1.5～2.5)μm;16S rDNA序列分析和同源性鉴定表明其为Acinetobacter oleivorans。(3)采用不同碳源(葡萄糖和乙酸钠)调控聚磷菌胞内辅酶Ⅰ及其对聚磷菌胞内多聚物代谢的影响。研究结果表明:乙酸钠系统中单位mol C下NADH含量为2.765μmol/g,NAD~+含量为3.028μmol/g,高于葡萄糖系统单位mol C下NADH含量的1.499μmol/g,NAD~+含量的1.587μmol/g,且乙酸钠系统NAD~+/NADH为1.095高于葡萄糖系统的1.058;乙酸钠系统中厌氧段PHB的合成量、poly-P水解量,好氧段PHB的消耗量、poly-P合成量高于葡萄糖系统,但葡萄糖系统中厌氧段糖原的消耗量高于乙酸钠系统,其中糖原的消耗可能与PHV的合成有关;乙酸钠系统的平均除磷效率为59.73%高于葡萄糖系统的51.41%。(4)采用不同浓度烟酸(0 mg/L、4 mg/L、8 mg/L、12 mg/L)调控聚磷菌胞内辅酶Ⅰ水平,研究其对聚磷菌胞内多聚物代谢的影响。不同浓度烟酸对聚磷菌胞内NADH和NAD~+水平产生一定的影响。不同烟酸浓度下胞内NADH平均含量分别为1.791μmol/g,2.392μmol/g,2.613μmol/g,2.633μmol/g,NAD~+平均含量为1.962μmol/g,2.780μmol/g,3.136μmol/g,3.346μmol/g;研究结果表明,随着烟酸浓度增加,NAD~+含量显著增加,NADH含量相对增加;随着烟酸浓度增加,NAD~+/NADH分别为1.095、1.162、1.200、1.270;随着NAD~+/NADH的升高,厌氧段PHB的合成量,poly-P水解量,好氧段PHB的消耗量,poly-P合成量不断增加,菌株的除磷效率不断增加,分别达到59.73%、70.48%、73.11%、76.13%。(5)胞内辅酶Ⅰ水平对聚磷菌除磷效率具有较大的影响。随着NAD~+/NADH不断升高,聚磷菌胞内PHB、poly-P的合成转化量越高,聚磷菌的除磷效率越高。NAD~+/NADH从1.058增加到1.270,菌株的除磷效率升高了24.72%。辅酶Ⅰ是聚磷菌胞内关键辅酶,该试验通过利用不同碳源和NAD~+前体物调控NADH和NAD~+的水平进一步调节聚磷菌胞内多聚物的代谢,有效提高了生物除磷的效率,该研究为强化生物除磷提供了新思路,具有一定的现实意义和理论价值。
Abstract
sheng wu chu lin yi zhi shi guo nei shui zhi ke xue he ji shu yan jiu de re dian wen ti ,diao kong ju lin jun bao nei duo ju wu dai xie shi di gao sheng wu chu lin xiao lv de you xiao tu jing 。yan xian an xian piao ling er he gan suan (fu mei Ⅰ,NADHhai yuan tai ,NAD~+yang hua tai )shi diao jie bao nei yang hua hai yuan fan ying ji ji chong yao de yin zi ,yin ci ,diao kong bao nei fu mei Ⅰde shui ping gai bian ju lin jun bao nei duo ju wu de dai xie shi di gao sheng wu chu lin xiao lv de yi chong xin sai lu 。ran er ,tong guo diao kong ju lin jun bao nei fu mei Ⅰshui ping diao jie bao nei duo ju wu dai xie de yan jiu jiao shao 。gai yan jiu tong guo ya yang /hao yang jiao ti yun hang de gong yi bing jie ge lan bai ban shai shua fa shai shua yi zhu ju lin jun 。zhen dui shai shua chu de ju lin jun ,tong guo gai bian tan yuan he tian jia bu tong nong du yan suan de wai yuan diao kong fang fa shi xian fu mei Ⅰde diao jie 。kao cha tan yuan he yan suan dui ju lin jun bao nei fu mei Ⅰde diao kong ji dui bao nei duo ju wu dai xie de ying xiang 。zhu yao yan jiu jie guo ru xia :(1)tong guo ya yang /hao yang jiao ti yun hang de gong yi kuai su fu ji ju lin jun bing cai yong ya yang /hao yang jiao ti de ping ban die dai pei yang fen li ju lin jun ,jie ge lan bai ban shai shua he yi ran ke li ji PHB(ju -β-qiang ji ding suan )ran se ,cong zhong shai shua chu ZWC-1、ZWC-2、ZWC-3、ZWC-4、ZWC-5、ZWC-6、ZWC-7、ZWC-8、ZWC-9、ZWC-10、ZWC-11、ZWC-12、ZWC-13、ZWC14gong 14zhu ju lin jun 。dui 14zhu ju lin jun de chu lin xing neng jin hang ce shi ,ce shi jie guo biao ming ,chu lin lv fen bie wei 44.5%、45.5%、77.5%、71.2%、47.3%、80.4%、76.6%、54.7%、76.2%、71.9%、43.6%、58.6%、75.6%、48.4%。gai shi yan shua ze ZWC-8jun zhu zuo wei yan jiu dui xiang 。(2)cai yong ge lan shi ran se 、sao miao dian jing guan cha ji 16S rDNAxu lie fen xi he tong yuan xing bi jiao dui ju lin jun ZWC-8jin hang jian ding 。ge lan shi ran se jie guo biao ming ,ZWC-8jun zhu wei ge lan shi yin xing jun ;sao miao dian jing jie guo biao ming ,jun zhu da xiao wei (0.4～0.5)μm×(1.5～2.5)μm;16S rDNAxu lie fen xi he tong yuan xing jian ding biao ming ji wei Acinetobacter oleivorans。(3)cai yong bu tong tan yuan (pu tao tang he yi suan na )diao kong ju lin jun bao nei fu mei Ⅰji ji dui ju lin jun bao nei duo ju wu dai xie de ying xiang 。yan jiu jie guo biao ming :yi suan na ji tong zhong chan wei mol Cxia NADHhan liang wei 2.765μmol/g,NAD~+han liang wei 3.028μmol/g,gao yu pu tao tang ji tong chan wei mol Cxia NADHhan liang de 1.499μmol/g,NAD~+han liang de 1.587μmol/g,ju yi suan na ji tong NAD~+/NADHwei 1.095gao yu pu tao tang ji tong de 1.058;yi suan na ji tong zhong ya yang duan PHBde ge cheng liang 、poly-Pshui jie liang ,hao yang duan PHBde xiao hao liang 、poly-Pge cheng liang gao yu pu tao tang ji tong ,dan pu tao tang ji tong zhong ya yang duan tang yuan de xiao hao liang gao yu yi suan na ji tong ,ji zhong tang yuan de xiao hao ke neng yu PHVde ge cheng you guan ;yi suan na ji tong de ping jun chu lin xiao lv wei 59.73%gao yu pu tao tang ji tong de 51.41%。(4)cai yong bu tong nong du yan suan (0 mg/L、4 mg/L、8 mg/L、12 mg/L)diao kong ju lin jun bao nei fu mei Ⅰshui ping ,yan jiu ji dui ju lin jun bao nei duo ju wu dai xie de ying xiang 。bu tong nong du yan suan dui ju lin jun bao nei NADHhe NAD~+shui ping chan sheng yi ding de ying xiang 。bu tong yan suan nong du xia bao nei NADHping jun han liang fen bie wei 1.791μmol/g,2.392μmol/g,2.613μmol/g,2.633μmol/g,NAD~+ping jun han liang wei 1.962μmol/g,2.780μmol/g,3.136μmol/g,3.346μmol/g;yan jiu jie guo biao ming ,sui zhao yan suan nong du zeng jia ,NAD~+han liang xian zhe zeng jia ,NADHhan liang xiang dui zeng jia ;sui zhao yan suan nong du zeng jia ,NAD~+/NADHfen bie wei 1.095、1.162、1.200、1.270;sui zhao NAD~+/NADHde sheng gao ,ya yang duan PHBde ge cheng liang ,poly-Pshui jie liang ,hao yang duan PHBde xiao hao liang ,poly-Pge cheng liang bu duan zeng jia ,jun zhu de chu lin xiao lv bu duan zeng jia ,fen bie da dao 59.73%、70.48%、73.11%、76.13%。(5)bao nei fu mei Ⅰshui ping dui ju lin jun chu lin xiao lv ju you jiao da de ying xiang 。sui zhao NAD~+/NADHbu duan sheng gao ,ju lin jun bao nei PHB、poly-Pde ge cheng zhuai hua liang yue gao ,ju lin jun de chu lin xiao lv yue gao 。NAD~+/NADHcong 1.058zeng jia dao 1.270,jun zhu de chu lin xiao lv sheng gao le 24.72%。fu mei Ⅰshi ju lin jun bao nei guan jian fu mei ,gai shi yan tong guo li yong bu tong tan yuan he NAD~+qian ti wu diao kong NADHhe NAD~+de shui ping jin yi bu diao jie ju lin jun bao nei duo ju wu de dai xie ,you xiao di gao le sheng wu chu lin de xiao lv ,gai yan jiu wei jiang hua sheng wu chu lin di gong le xin sai lu ,ju you yi ding de xian shi yi yi he li lun jia zhi 。
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标签：生物除磷论文; 辅酶论文; 糖原论文; 安徽建筑大学2019-07-16论文;