本文主要研究内容
作者董鹏飞（2019）在《光交联胶原蛋白载药纳米纤维的可控性释放研究》一文中研究指出：胶原蛋白(COL)广泛分布于动物骨骼,肌腱,软骨和皮肤中,是细胞外基质(ECM)的主要成分。因此具有优异的生物相容性和生物降解性被广泛用于伤口敷料、药物载体和组织工程。静电纺载药胶原蛋白纳米纤维具有高的比表面积和孔隙率,因此有较高的药物装载效率。但由于其在水中高的溶解性易造成药物的突释,需要提高其耐水性。为避免化学交联剂在生物医学领域的副作用,本文分别通过紫外交联胶原蛋白和乙基纤维素共纺以改善药物突释现象。第一部分利用甲基丙烯酸酐对胶原蛋白进行改性,使其侧链引入C=C双键以形成乙烯基胶原蛋白(CMA)。采用静电纺丝制备了CMA纳米纤维,再用紫外辐射诱导其自由基聚合形成胶原蛋白分子交联网络以提高交联度。用SEM和FTIR对紫外交联的改性胶原蛋白(UV-CMA)纳米纤维的形貌和化学结构进行了表征。紫外交联没有破坏纤维的形貌并提高了耐水性和机械性能。UV-CMA纳米纤维吸水率最低,比纯胶原纤维降低了11.78%,抗拉伸强度比纯胶原纤维提高了两倍为2MPa。5-氟尿嘧啶(5-FU)作为模型药物添加到CMA多孔纳米纤维中用于评估药物输送系统,再通过紫外辐射交联以减弱药物突释。体外释放研究表明,紫外辐射交联后,载药CMA纳米纤维有效控制了药物的释放,2h内仅释放药物总量的60%,并持续释放至10h,而纯胶原纤维则全部突释。药物释放机制为Fickian扩散机制。第二部分制备了胶原蛋白/乙基纤维素(EC)载药纳米纤维膜。通过SEM和TEM观察到共纺纤维形貌光滑完整,没有明显的相分离现象。FTIR表明COL和EC分子间以氢键相连,两相分子结构没有任何破坏。随着EC含量的增加,纤维的平均直径降低,水接触角增加,抗水性能增强。当EC含量为50%时,共混纤维表层的COL含量仅为15.3%,水接触角为133°,浸水24h后的溶失率仅为2.9%。当EC含量为20%时,共混纤维浸水24h后仍能看出纤维的轮廓,水接触角降低到106°。药物均匀分散在混纺纤维中,纤维表面和内部没有明显的药物结晶颗粒。随着药物含量的增加,共混纤维的平均直径有所降低。当EC占比50%时,混纺纤维12h释放药物总量的80.16%,持续释放时间到80h,而纯胶原纤维2h全部突释,提高了药物的利用率。同时可以通过添加EC含量的控制,形成释药时间和释药量可控的释药过程,这对疾病的治疗非常重要。
Abstract
jiao yuan dan bai (COL)an fan fen bu yu dong wu gu ge ,ji jian ,ruan gu he pi fu zhong ,shi xi bao wai ji zhi (ECM)de zhu yao cheng fen 。yin ci ju you you yi de sheng wu xiang rong xing he sheng wu jiang jie xing bei an fan yong yu shang kou fu liao 、yao wu zai ti he zu zhi gong cheng 。jing dian fang zai yao jiao yuan dan bai na mi qian wei ju you gao de bi biao mian ji he kong xi lv ,yin ci you jiao gao de yao wu zhuang zai xiao lv 。dan you yu ji zai shui zhong gao de rong jie xing yi zao cheng yao wu de tu shi ,xu yao di gao ji nai shui xing 。wei bi mian hua xue jiao lian ji zai sheng wu yi xue ling yu de fu zuo yong ,ben wen fen bie tong guo zi wai jiao lian jiao yuan dan bai he yi ji qian wei su gong fang yi gai shan yao wu tu shi xian xiang 。di yi bu fen li yong jia ji bing xi suan gan dui jiao yuan dan bai jin hang gai xing ,shi ji ce lian yin ru C=Cshuang jian yi xing cheng yi xi ji jiao yuan dan bai (CMA)。cai yong jing dian fang si zhi bei le CMAna mi qian wei ,zai yong zi wai fu she you dao ji zi you ji ju ge xing cheng jiao yuan dan bai fen zi jiao lian wang lao yi di gao jiao lian du 。yong SEMhe FTIRdui zi wai jiao lian de gai xing jiao yuan dan bai (UV-CMA)na mi qian wei de xing mao he hua xue jie gou jin hang le biao zheng 。zi wai jiao lian mei you po huai qian wei de xing mao bing di gao le nai shui xing he ji xie xing neng 。UV-CMAna mi qian wei xi shui lv zui di ,bi chun jiao yuan qian wei jiang di le 11.78%,kang la shen jiang du bi chun jiao yuan qian wei di gao le liang bei wei 2MPa。5-fu niao mi ding (5-FU)zuo wei mo xing yao wu tian jia dao CMAduo kong na mi qian wei zhong yong yu ping gu yao wu shu song ji tong ,zai tong guo zi wai fu she jiao lian yi jian ruo yao wu tu shi 。ti wai shi fang yan jiu biao ming ,zi wai fu she jiao lian hou ,zai yao CMAna mi qian wei you xiao kong zhi le yao wu de shi fang ,2hnei jin shi fang yao wu zong liang de 60%,bing chi xu shi fang zhi 10h,er chun jiao yuan qian wei ze quan bu tu shi 。yao wu shi fang ji zhi wei Fickiankuo san ji zhi 。di er bu fen zhi bei le jiao yuan dan bai /yi ji qian wei su (EC)zai yao na mi qian wei mo 。tong guo SEMhe TEMguan cha dao gong fang qian wei xing mao guang hua wan zheng ,mei you ming xian de xiang fen li xian xiang 。FTIRbiao ming COLhe ECfen zi jian yi qing jian xiang lian ,liang xiang fen zi jie gou mei you ren he po huai 。sui zhao EChan liang de zeng jia ,qian wei de ping jun zhi jing jiang di ,shui jie chu jiao zeng jia ,kang shui xing neng zeng jiang 。dang EChan liang wei 50%shi ,gong hun qian wei biao ceng de COLhan liang jin wei 15.3%,shui jie chu jiao wei 133°,jin shui 24hhou de rong shi lv jin wei 2.9%。dang EChan liang wei 20%shi ,gong hun qian wei jin shui 24hhou reng neng kan chu qian wei de lun kuo ,shui jie chu jiao jiang di dao 106°。yao wu jun yun fen san zai hun fang qian wei zhong ,qian wei biao mian he nei bu mei you ming xian de yao wu jie jing ke li 。sui zhao yao wu han liang de zeng jia ,gong hun qian wei de ping jun zhi jing you suo jiang di 。dang ECzhan bi 50%shi ,hun fang qian wei 12hshi fang yao wu zong liang de 80.16%,chi xu shi fang shi jian dao 80h,er chun jiao yuan qian wei 2hquan bu tu shi ,di gao le yao wu de li yong lv 。tong shi ke yi tong guo tian jia EChan liang de kong zhi ,xing cheng shi yao shi jian he shi yao liang ke kong de shi yao guo cheng ,zhe dui ji bing de zhi liao fei chang chong yao 。
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论文作者分别是来自天津工业大学的董鹏飞，发表于刊物天津工业大学2019-07-05论文，是一篇关于胶原蛋白论文,乙基纤维素论文,静电纺丝论文,纤维载药论文,紫外交联论文,天津工业大学2019-07-05论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自天津工业大学2019-07-05论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：胶原蛋白论文; 乙基纤维素论文; 静电纺丝论文; 纤维载药论文; 紫外交联论文; 天津工业大学2019-07-05论文;