本文主要研究内容
作者孙玮玮（2019）在《鸡gga-miR-1306-5p、gga-miR-155靶向调控Tollip抗沙门氏菌的作用机制》一文中研究指出：肠炎沙门氏菌(Salmonella enterica,SE)被公认为是一种重要的食源性病原菌,对家禽的公共健康造成严重威胁,因此造成巨大的经济损失。尽管家禽养殖业中SE污染可以通过多种措施进行有效控制,但是目前还是有大量的SE污染发生。这主要的原因就是SE目前在预防和治疗措施方面存在许多缺陷,其中SE对多种抗菌剂的耐药性就是治疗疾病的重要难题及挑战。因此,针对SE的感染,开发新型的预防和治疗措施是非常必要的。先天性免疫反应是宿主抵抗侵入的感染性病原体的第一层防线。对病原微生物的识别是激活宿主先天免疫反应的必要步骤,主要依赖于多种种系编码的病原识别受体。众所周知,TLR是最为广泛研究的病原识别受体,是宿主清除入侵性病原体所必须的关键性调节介质。Toll样互作蛋白(Toll interactingprotein,Tollip),是一种TLR相关信号蛋白,是TLR信号通路中一个内源性调节因子。越来越多的证据显示Tollip通过抑制白介素1受体相关激酶IRAK-1的活性,来维持免疫稳态,调控炎症反应过程中MyD88依赖性NF-κB信号通路的激活。MicroRNA(miRNA)是一类小、非编码,长度约为18-25个核苷酸的RNA分子,可以通过降低转录后基因水平的表达,来调节一系列的生物学过程。据报道,高达60%的蛋白编码基因经预测在一定程度上由miRNA调节。而且miRNA可以通过绑定在靶基因的3’-非翻译区(3’-UTR),引起基因mRNA水平降解或蛋白翻译。在本研究中,我们的数据显示鸡Gallus gallus microRNA-1306-5p(gga-miR-1306-5p)和Gallus gallus microRNA-155(gga-miR-155)在SE感染后的各种组织(脾脏、法氏囊、盲肠扁桃体、盲肠),以及LPS刺激后的HD11细胞中,表达水平均显著性上调。然后,我们进行了双荧光素酶报告基因检测,结果显示gga-miR-1306-5p及gga-miR-155 均靶向调控Tollip基因;当gga-miR-1306-5p及gga-miR-155过表达时会显著性降低Tollip在蛋白及mRNA水平的表达;当gga-nmiR-1306-5p及gga-miR-155表达抑制时则会起相反的效果,增加Tollip在蛋白及mRNA上的表达水平。而且,gga-miR-1306-5p及gga-miR-155的过表达会诱导下游促炎性细胞因子,包括NF-κB,TNF-αt,IL-6和IL-1β的释放;当靶基因Tollip沉默时对下游促炎因子的影响与gga-miR-1306-5p及gga-miR-155过表达时的效果一致。总的来说,病原菌及LPS刺激引起的gga-miR-1306-5p及gga-miR-155上调,抑制了靶基因Tollip的表达,进而刺激了-下游促炎因子的产生。我们的数据不仅丰富了 SE感染过程中miRNA的具体作用,而且对理解鸡先天免疫反应中的防御机制提供了新的视角。
Abstract
chang yan sha men shi jun (Salmonella enterica,SE)bei gong ren wei shi yi chong chong yao de shi yuan xing bing yuan jun ,dui jia qin de gong gong jian kang zao cheng yan chong wei xie ,yin ci zao cheng ju da de jing ji sun shi 。jin guan jia qin yang shi ye zhong SEwu ran ke yi tong guo duo chong cuo shi jin hang you xiao kong zhi ,dan shi mu qian hai shi you da liang de SEwu ran fa sheng 。zhe zhu yao de yuan yin jiu shi SEmu qian zai yu fang he zhi liao cuo shi fang mian cun zai hu duo que xian ,ji zhong SEdui duo chong kang jun ji de nai yao xing jiu shi zhi liao ji bing de chong yao nan ti ji tiao zhan 。yin ci ,zhen dui SEde gan ran ,kai fa xin xing de yu fang he zhi liao cuo shi shi fei chang bi yao de 。xian tian xing mian yi fan ying shi su zhu di kang qin ru de gan ran xing bing yuan ti de di yi ceng fang xian 。dui bing yuan wei sheng wu de shi bie shi ji huo su zhu xian tian mian yi fan ying de bi yao bu zhou ,zhu yao yi lai yu duo chong chong ji bian ma de bing yuan shi bie shou ti 。zhong suo zhou zhi ,TLRshi zui wei an fan yan jiu de bing yuan shi bie shou ti ,shi su zhu qing chu ru qin xing bing yuan ti suo bi xu de guan jian xing diao jie jie zhi 。Tollyang hu zuo dan bai (Toll interactingprotein,Tollip),shi yi chong TLRxiang guan xin hao dan bai ,shi TLRxin hao tong lu zhong yi ge nei yuan xing diao jie yin zi 。yue lai yue duo de zheng ju xian shi Tolliptong guo yi zhi bai jie su 1shou ti xiang guan ji mei IRAK-1de huo xing ,lai wei chi mian yi wen tai ,diao kong yan zheng fan ying guo cheng zhong MyD88yi lai xing NF-κBxin hao tong lu de ji huo 。MicroRNA(miRNA)shi yi lei xiao 、fei bian ma ,chang du yao wei 18-25ge he gan suan de RNAfen zi ,ke yi tong guo jiang di zhuai lu hou ji yin shui ping de biao da ,lai diao jie yi ji lie de sheng wu xue guo cheng 。ju bao dao ,gao da 60%de dan bai bian ma ji yin jing yu ce zai yi ding cheng du shang you miRNAdiao jie 。er ju miRNAke yi tong guo bang ding zai ba ji yin de 3’-fei fan yi ou (3’-UTR),yin qi ji yin mRNAshui ping jiang jie huo dan bai fan yi 。zai ben yan jiu zhong ,wo men de shu ju xian shi ji Gallus gallus microRNA-1306-5p(gga-miR-1306-5p)he Gallus gallus microRNA-155(gga-miR-155)zai SEgan ran hou de ge chong zu zhi (pi zang 、fa shi nang 、mang chang bian tao ti 、mang chang ),yi ji LPSci ji hou de HD11xi bao zhong ,biao da shui ping jun xian zhe xing shang diao 。ran hou ,wo men jin hang le shuang ying guang su mei bao gao ji yin jian ce ,jie guo xian shi gga-miR-1306-5pji gga-miR-155 jun ba xiang diao kong Tollipji yin ;dang gga-miR-1306-5pji gga-miR-155guo biao da shi hui xian zhe xing jiang di Tollipzai dan bai ji mRNAshui ping de biao da ;dang gga-nmiR-1306-5pji gga-miR-155biao da yi zhi shi ze hui qi xiang fan de xiao guo ,zeng jia Tollipzai dan bai ji mRNAshang de biao da shui ping 。er ju ,gga-miR-1306-5pji gga-miR-155de guo biao da hui you dao xia you cu yan xing xi bao yin zi ,bao gua NF-κB,TNF-αt,IL-6he IL-1βde shi fang ;dang ba ji yin Tollipchen mo shi dui xia you cu yan yin zi de ying xiang yu gga-miR-1306-5pji gga-miR-155guo biao da shi de xiao guo yi zhi 。zong de lai shui ,bing yuan jun ji LPSci ji yin qi de gga-miR-1306-5pji gga-miR-155shang diao ,yi zhi le ba ji yin Tollipde biao da ,jin er ci ji le -xia you cu yan yin zi de chan sheng 。wo men de shu ju bu jin feng fu le SEgan ran guo cheng zhong miRNAde ju ti zuo yong ,er ju dui li jie ji xian tian mian yi fan ying zhong de fang yu ji zhi di gong le xin de shi jiao 。
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论文作者分别是来自中国农业科学院的孙玮玮，发表于刊物中国农业科学院2019-08-21论文，是一篇关于沙门氏菌论文,促炎性细胞因子论文,中国农业科学院2019-08-21论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自中国农业科学院2019-08-21论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。