本文主要研究内容
作者蒋敏（2019）在《寄主植物挥发物对莲草直胸跳甲趋性行为和生长繁殖的影响》一文中研究指出：植物挥发物在植物与昆虫的相互作用间起着重要作用。莲草直胸跳甲作为空心莲子草的专食性天敌,主要通过嗅觉感受器利用寄主植物释放的挥发物进行寻找、识别,从而达到取食的目的。本文通过研究不同处理的空心莲子草的挥发物成分,及其芳香性挥发物邻苯二甲酸二丁酯和水杨酸甲酯对莲草直胸跳甲生物活性和趋性行为以及生长繁殖的影响,明确了单一挥发物对莲草直胸跳甲的作用。其主要研究结果如下:(1)不同处理的空心莲子草挥发物本研究采用固相微萃取的方法,利用GC-MS-MS对不同处理的空心莲子草挥发物进行测定。结果显示:在处理1、6、12、24、48 h后,健康无损害的空心莲子草共释放41种化合物,且在处理12 h后释放的挥发物种类最多;机械损伤后所释放的挥发物共检测出17种,且在损伤1 h后所释放的挥发物最多;1龄幼虫取食后共释放挥发物28种,且在取食处理24 h后测得了最多的挥发物成分;2龄幼虫取食为害后共释放挥发物43种,其中在取食处理48 h后释放的挥发物最多;3龄幼虫为害后,相比其他处理,释放出最多的挥发物,共74种,其中检测到最多挥发物成分的时间是取食24 h后;雌成虫取食后,空心莲子草共释放出28种化合物,且释放挥发物成分最多的时间是取食12 h和24 h后,但挥发物的成分及含量有差异;雄成虫取食后,空心莲子草共释放出28种挥发物,其中检测出最多化合物的时间为取食12 h和48 h,但挥发物的成分及含量有差异。(2)邻苯二甲酸二丁酯和水杨酸甲酯对莲草直胸跳甲的生物活性和趋性行为影响本研究选择两种芳香挥发物邻苯二甲酸二丁酯和水杨酸甲酯,测定了其对莲草直胸跳甲2龄幼虫和成虫的活性影响以及不同浓度对莲草直胸跳甲雌、雄成虫的趋性行为影响。最终测得邻苯二甲酸二丁酯对莲草直胸跳甲2龄幼虫和成虫的亚致死计量LD10、LD20和LD50分别为0.7μmol/m L、0.9μmol/m L、1.2μmol/m L和1.7μmol/m L、2.9μmol/m L、8.0μmol/m L;水杨酸甲酯对莲草直胸跳甲2龄幼虫和成虫的亚致死计量LD10、LD20和LD50分别为0.8μmol/m L、1.2μmol/m L、2.2μmol/m L和2.9μmol/m L、4.1μmol/m L、7.9μmol/m L。邻苯二甲酸二丁酯稀释100后,对莲草直胸跳甲雌、雄成虫均具有显著的忌避作用,原药及稀释1000后,均无显著影响;而水杨酸甲酯只有在原药时对雌成虫有忌避作用,其他浓度无显著影响,雄成虫对三种浓度均无显著反应。(3)邻苯二甲酸二丁酯和水杨酸甲酯对莲草直胸跳甲生长繁殖的影响本研究建立了取食不同浓度邻苯二甲酸二丁酯和水杨酸甲酯浸液处理的空心莲子草后,莲草直胸跳甲的种群生命表。结果表明:相比对照组,莲草直胸跳甲在0.71μmol/m L邻苯二甲酸二丁酯的影响下,发育历期延长且差异显著,雌、雄成虫及总虫寿命以及雌虫产卵期和产卵量均有延长但无差异;而在1.75μmol/m L浓度影响下,莲草直胸跳甲发育历期延长且差异显著,雌、雄成虫以及雌虫产卵期和产卵量均有延长但无差异,但总虫寿命缩短且差异显著。相比对照组,莲草直胸跳甲在0.82μmol/m L水杨酸甲酯的影响下,发育历期缩短且差异显著,雌、雄成虫及总虫寿命以及雌虫产卵期和产卵量均缩短但无差异;而在0.82μmol/m L水杨酸甲酯浓度影响下,莲草直胸跳甲发育历期无显著差异,总虫、雌、雄成虫以及雌虫产卵期均缩短但无差异,产卵量增加。本文通过研究寄主植物特异性挥发物对莲草直胸跳甲趋性行为和生长繁殖的影响,探索食性专一的植食性昆虫对其寄主的选择关系,有利于今后生物防治领域中寄主专一性测定的理论与实践,有助于阐明昆虫与植物之间的协同进化关系,对杂草生物防治具有较好的指导意义。
Abstract
zhi wu hui fa wu zai zhi wu yu kun chong de xiang hu zuo yong jian qi zhao chong yao zuo yong 。lian cao zhi xiong tiao jia zuo wei kong xin lian zi cao de zhuan shi xing tian di ,zhu yao tong guo xiu jiao gan shou qi li yong ji zhu zhi wu shi fang de hui fa wu jin hang xun zhao 、shi bie ,cong er da dao qu shi de mu de 。ben wen tong guo yan jiu bu tong chu li de kong xin lian zi cao de hui fa wu cheng fen ,ji ji fang xiang xing hui fa wu lin ben er jia suan er ding zhi he shui yang suan jia zhi dui lian cao zhi xiong tiao jia sheng wu huo xing he qu xing hang wei yi ji sheng chang fan shi de ying xiang ,ming que le chan yi hui fa wu dui lian cao zhi xiong tiao jia de zuo yong 。ji zhu yao yan jiu jie guo ru xia :(1)bu tong chu li de kong xin lian zi cao hui fa wu ben yan jiu cai yong gu xiang wei cui qu de fang fa ,li yong GC-MS-MSdui bu tong chu li de kong xin lian zi cao hui fa wu jin hang ce ding 。jie guo xian shi :zai chu li 1、6、12、24、48 hhou ,jian kang mo sun hai de kong xin lian zi cao gong shi fang 41chong hua ge wu ,ju zai chu li 12 hhou shi fang de hui fa wu chong lei zui duo ;ji xie sun shang hou suo shi fang de hui fa wu gong jian ce chu 17chong ,ju zai sun shang 1 hhou suo shi fang de hui fa wu zui duo ;1ling you chong qu shi hou gong shi fang hui fa wu 28chong ,ju zai qu shi chu li 24 hhou ce de le zui duo de hui fa wu cheng fen ;2ling you chong qu shi wei hai hou gong shi fang hui fa wu 43chong ,ji zhong zai qu shi chu li 48 hhou shi fang de hui fa wu zui duo ;3ling you chong wei hai hou ,xiang bi ji ta chu li ,shi fang chu zui duo de hui fa wu ,gong 74chong ,ji zhong jian ce dao zui duo hui fa wu cheng fen de shi jian shi qu shi 24 hhou ;ci cheng chong qu shi hou ,kong xin lian zi cao gong shi fang chu 28chong hua ge wu ,ju shi fang hui fa wu cheng fen zui duo de shi jian shi qu shi 12 hhe 24 hhou ,dan hui fa wu de cheng fen ji han liang you cha yi ;xiong cheng chong qu shi hou ,kong xin lian zi cao gong shi fang chu 28chong hui fa wu ,ji zhong jian ce chu zui duo hua ge wu de shi jian wei qu shi 12 hhe 48 h,dan hui fa wu de cheng fen ji han liang you cha yi 。(2)lin ben er jia suan er ding zhi he shui yang suan jia zhi dui lian cao zhi xiong tiao jia de sheng wu huo xing he qu xing hang wei ying xiang ben yan jiu shua ze liang chong fang xiang hui fa wu lin ben er jia suan er ding zhi he shui yang suan jia zhi ,ce ding le ji dui lian cao zhi xiong tiao jia 2ling you chong he cheng chong de huo xing ying xiang yi ji bu tong nong du dui lian cao zhi xiong tiao jia ci 、xiong cheng chong de qu xing hang wei ying xiang 。zui zhong ce de lin ben er jia suan er ding zhi dui lian cao zhi xiong tiao jia 2ling you chong he cheng chong de ya zhi si ji liang LD10、LD20he LD50fen bie wei 0.7μmol/m L、0.9μmol/m L、1.2μmol/m Lhe 1.7μmol/m L、2.9μmol/m L、8.0μmol/m L;shui yang suan jia zhi dui lian cao zhi xiong tiao jia 2ling you chong he cheng chong de ya zhi si ji liang LD10、LD20he LD50fen bie wei 0.8μmol/m L、1.2μmol/m L、2.2μmol/m Lhe 2.9μmol/m L、4.1μmol/m L、7.9μmol/m L。lin ben er jia suan er ding zhi xi shi 100hou ,dui lian cao zhi xiong tiao jia ci 、xiong cheng chong jun ju you xian zhe de ji bi zuo yong ,yuan yao ji xi shi 1000hou ,jun mo xian zhe ying xiang ;er shui yang suan jia zhi zhi you zai yuan yao shi dui ci cheng chong you ji bi zuo yong ,ji ta nong du mo xian zhe ying xiang ,xiong cheng chong dui san chong nong du jun mo xian zhe fan ying 。(3)lin ben er jia suan er ding zhi he shui yang suan jia zhi dui lian cao zhi xiong tiao jia sheng chang fan shi de ying xiang ben yan jiu jian li le qu shi bu tong nong du lin ben er jia suan er ding zhi he shui yang suan jia zhi jin ye chu li de kong xin lian zi cao hou ,lian cao zhi xiong tiao jia de chong qun sheng ming biao 。jie guo biao ming :xiang bi dui zhao zu ,lian cao zhi xiong tiao jia zai 0.71μmol/m Llin ben er jia suan er ding zhi de ying xiang xia ,fa yo li ji yan chang ju cha yi xian zhe ,ci 、xiong cheng chong ji zong chong shou ming yi ji ci chong chan luan ji he chan luan liang jun you yan chang dan mo cha yi ;er zai 1.75μmol/m Lnong du ying xiang xia ,lian cao zhi xiong tiao jia fa yo li ji yan chang ju cha yi xian zhe ,ci 、xiong cheng chong yi ji ci chong chan luan ji he chan luan liang jun you yan chang dan mo cha yi ,dan zong chong shou ming su duan ju cha yi xian zhe 。xiang bi dui zhao zu ,lian cao zhi xiong tiao jia zai 0.82μmol/m Lshui yang suan jia zhi de ying xiang xia ,fa yo li ji su duan ju cha yi xian zhe ,ci 、xiong cheng chong ji zong chong shou ming yi ji ci chong chan luan ji he chan luan liang jun su duan dan mo cha yi ;er zai 0.82μmol/m Lshui yang suan jia zhi nong du ying xiang xia ,lian cao zhi xiong tiao jia fa yo li ji mo xian zhe cha yi ,zong chong 、ci 、xiong cheng chong yi ji ci chong chan luan ji jun su duan dan mo cha yi ,chan luan liang zeng jia 。ben wen tong guo yan jiu ji zhu zhi wu te yi xing hui fa wu dui lian cao zhi xiong tiao jia qu xing hang wei he sheng chang fan shi de ying xiang ,tan suo shi xing zhuan yi de zhi shi xing kun chong dui ji ji zhu de shua ze guan ji ,you li yu jin hou sheng wu fang zhi ling yu zhong ji zhu zhuan yi xing ce ding de li lun yu shi jian ,you zhu yu chan ming kun chong yu zhi wu zhi jian de xie tong jin hua guan ji ,dui za cao sheng wu fang zhi ju you jiao hao de zhi dao yi yi 。
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论文作者分别是来自福建农林大学的蒋敏，发表于刊物福建农林大学2019-08-30论文，是一篇关于莲草直胸跳甲论文,空心莲子草论文,植物挥发性物质论文,趋性行为论文,生长繁殖论文,福建农林大学2019-08-30论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自福建农林大学2019-08-30论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：莲草直胸跳甲论文; 空心莲子草论文; 植物挥发性物质论文; 趋性行为论文; 生长繁殖论文; 福建农林大学2019-08-30论文;