本文主要研究内容
作者陈永卓（2019）在《噪声对黑线毛足鼠焦虑行为的影响及其分子机制》一文中研究指出：高速公路噪声作为一种环境刺激,可以激活HPA轴,CRHR1受体在HPA轴对外界刺激产生应答过程中起关键作用。我们探究了急慢性噪声对黑线毛足鼠行为及下丘脑中CRHR1表达的影响。将40只成年黑线毛足鼠随机分为以下8组,每组5只:(1)雌性急性对照(F AC,Female Acute Control)、(2)雌性急性85dBA处理(F A85,Female Acute 85dBA exposure);(3)雄性急性对照(M AC,Male Acute Control,)、(4)雄性急性85dBA处理(M A85,Male Acute 85dBA exposure);(5)雌性慢性对照(F CC,Female Chronic Control)(6)雌性慢性85dBA处理(F C85,Female Chronic 85dBA exposure);(7)雄性慢性对照(M CC,Male Chronic Control)、(8)雄性慢性85dBA处理(M C85,Male Chronic 85dBA exposure)。急性处理组使用85dBA高速公路噪声处理4h,慢性处理组在相同噪声条件下每天4h,持续30天。下丘脑中CRHR1表达量使用实时荧光定量PCR检测。通过红外旷场实验、明暗箱实验以及高架十字迷宫实验检测噪声对黑线毛足鼠行为的影响,急性噪声处理后行为学实验仅进行一次,慢性噪声处理后行为学实验每5天进行一次。急性噪声使雌雄黑线毛足鼠产生轻微的焦虑行为,对下丘脑CRHR1的表达无显著影响,慢性噪声使雄性个体表现出一定程度异常冲动和亢奋的低焦虑行为并使下丘脑中CRHR1表达显著降低,但慢性噪声对雌性行为的影响要小于雄性个体且对雌性下丘脑中CRHR1表达没有显著影响。相关性分析表明,下丘脑CRHR1表达量与中央区域停留时间呈极显著负相关r=-0.579,P<0.01,与中央区域运动距离呈显著负相关r=-0.466,P=0.014,但是与其他行为学指标的相关性不显著。本研究表明,急慢性噪声均可以导致黑线毛足鼠行为变化,并且慢性噪声可以导致雄性个体下丘脑CRHR1表达降低,本研究为掌握CRHR1在噪声调控动物行为中的作用效应提供理论基础。
Abstract
gao su gong lu zao sheng zuo wei yi chong huan jing ci ji ,ke yi ji huo HPAzhou ,CRHR1shou ti zai HPAzhou dui wai jie ci ji chan sheng ying da guo cheng zhong qi guan jian zuo yong 。wo men tan jiu le ji man xing zao sheng dui hei xian mao zu shu hang wei ji xia qiu nao zhong CRHR1biao da de ying xiang 。jiang 40zhi cheng nian hei xian mao zu shu sui ji fen wei yi xia 8zu ,mei zu 5zhi :(1)ci xing ji xing dui zhao (F AC,Female Acute Control)、(2)ci xing ji xing 85dBAchu li (F A85,Female Acute 85dBA exposure);(3)xiong xing ji xing dui zhao (M AC,Male Acute Control,)、(4)xiong xing ji xing 85dBAchu li (M A85,Male Acute 85dBA exposure);(5)ci xing man xing dui zhao (F CC,Female Chronic Control)(6)ci xing man xing 85dBAchu li (F C85,Female Chronic 85dBA exposure);(7)xiong xing man xing dui zhao (M CC,Male Chronic Control)、(8)xiong xing man xing 85dBAchu li (M C85,Male Chronic 85dBA exposure)。ji xing chu li zu shi yong 85dBAgao su gong lu zao sheng chu li 4h,man xing chu li zu zai xiang tong zao sheng tiao jian xia mei tian 4h,chi xu 30tian 。xia qiu nao zhong CRHR1biao da liang shi yong shi shi ying guang ding liang PCRjian ce 。tong guo gong wai kuang chang shi yan 、ming an xiang shi yan yi ji gao jia shi zi mi gong shi yan jian ce zao sheng dui hei xian mao zu shu hang wei de ying xiang ,ji xing zao sheng chu li hou hang wei xue shi yan jin jin hang yi ci ,man xing zao sheng chu li hou hang wei xue shi yan mei 5tian jin hang yi ci 。ji xing zao sheng shi ci xiong hei xian mao zu shu chan sheng qing wei de jiao lv hang wei ,dui xia qiu nao CRHR1de biao da mo xian zhe ying xiang ,man xing zao sheng shi xiong xing ge ti biao xian chu yi ding cheng du yi chang chong dong he kang fen de di jiao lv hang wei bing shi xia qiu nao zhong CRHR1biao da xian zhe jiang di ,dan man xing zao sheng dui ci xing hang wei de ying xiang yao xiao yu xiong xing ge ti ju dui ci xing xia qiu nao zhong CRHR1biao da mei you xian zhe ying xiang 。xiang guan xing fen xi biao ming ,xia qiu nao CRHR1biao da liang yu zhong yang ou yu ting liu shi jian cheng ji xian zhe fu xiang guan r=-0.579,P<0.01,yu zhong yang ou yu yun dong ju li cheng xian zhe fu xiang guan r=-0.466,P=0.014,dan shi yu ji ta hang wei xue zhi biao de xiang guan xing bu xian zhe 。ben yan jiu biao ming ,ji man xing zao sheng jun ke yi dao zhi hei xian mao zu shu hang wei bian hua ,bing ju man xing zao sheng ke yi dao zhi xiong xing ge ti xia qiu nao CRHR1biao da jiang di ,ben yan jiu wei zhang wo CRHR1zai zao sheng diao kong dong wu hang wei zhong de zuo yong xiao ying di gong li lun ji chu 。
论文参考文献
论文详细介绍
论文作者分别是来自曲阜师范大学的陈永卓，发表于刊物曲阜师范大学2019-10-08论文，是一篇关于黑线毛足鼠论文,高速公路噪声论文,焦虑行为论文,曲阜师范大学2019-10-08论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自曲阜师范大学2019-10-08论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：黑线毛足鼠论文; 高速公路噪声论文; 焦虑行为论文; 曲阜师范大学2019-10-08论文;