本文主要研究内容
作者王喆（2019）在《OsDLT基因调控的水稻抗虫防御信号网络解析》一文中研究指出：油菜素内酯(brassinosteroids,BRs)是一种多羟基甾醇类化合物,具有极高的生物活性是世界公认的第六大植物激素。其参与调控植物生长发育的许多过程,同时在植物响应生物和非生物胁迫中发挥作用。水稻(Oryza sativa)是世界上最重要的粮食作物之一,其产量和质量关乎绝大部分人口的生存,而褐飞虱(Nilaparvata lugens St?l,BPH)作为水稻单食性害虫是水稻生产中重要的害虫之一。本文从一个油菜素内酯信号通路下游的转录因子OsDLT(DWARF AND LOW-TILLERING)出发,研究其调控的水稻抗虫防御信号网络,结果如下:(1)相比于相同发育阶段的野生型水稻,突变体水稻dlt对褐飞虱的取食危害具有更强的耐受性。同时,不同发育阶段的野生型水稻和48日龄dlt水稻的褐飞虱选择性实验表明褐飞虱更喜爱危害各个发育阶段的野生型水稻。(2)OsDLT基因过量表达增加了水稻对褐飞虱的敏感性,与野生型水稻相比褐飞虱雌成虫更喜欢在过量表达OsDLT取食和产卵。取食过量表达OsDLT水稻的初羽化雌成虫分泌的蜜露量显著增加;褐飞虱若虫发育历期显著缩短,F1代单雌产卵量和卵孵化率显著上升。(3)与未处理9311相比,褐飞虱雌成虫更趋向于在24-表油菜素内酯(24-epicastasterone,ECS)处理的9311水稻上取食和产卵;而褐飞虱雌成虫对未处理和ECS处理的OsDLT功能确实突变体水稻dlt之间无取食和产卵偏好。丙环唑(Propiconazole,PCZ)是植物BR合成抑制剂,与未处理9311相比褐飞虱雌成虫在PCZ处理9311水稻上的取食和产卵量显著减少;而与未处理的dlt突变体水稻相比,褐飞虱雌成虫在PCZ处理的dlt水稻上的取食和产卵量显著增加。与未处理的9311水稻相比,经Bikinin处理的9311水稻对褐飞虱雌成虫取食和产卵的敏感性显著地降低;而dlt突变体,Bikinin处理增加褐飞虱雌成虫取食和产卵敏感性。(4)转录组数据分析后得到7196个差异表达基因。对褐飞虱取食处理9311水稻(WB)和dlt水稻(dB)出现的差异表达基因进行分析,茉莉酸途径中总共有18个基因出现了上调,占该途径差异表达基因的81.83%。水杨酸途径中总共有15个基因出现差异表达,其中11个基因出现上调占水杨酸途径差异表达基因的73.33%。18个防御相关的WRKY家族占WRKY家族差异表达基因的85.71%,9个ERF家族差异表达基因全部上调。3个病程相关蛋白蛋白、5个几丁质酶出现了显著上调。(5)通过构建酵母双杂交体系证明了OsDLT蛋白自身不具有自激活并筛选出3个和OsDLT蛋白具有相互作用的蛋白编码基因。之后克隆并构建了4个茉莉酸抑制子JAZ家族基因的激活质粒,通过酵母双杂交证明OsJAZ6与OsDLT蛋白可以产生相互作用。本文研究表明,OsDLT在褐飞虱诱导的防御反应中发挥重要作用,该基因负调控水稻对褐飞虱的抗性;OsDLT可能是油菜素内酯途径与茉莉酸途径互作的重要因子,在调控水稻的抗虫防御反应。
Abstract
you cai su nei zhi (brassinosteroids,BRs)shi yi chong duo qiang ji zai chun lei hua ge wu ,ju you ji gao de sheng wu huo xing shi shi jie gong ren de di liu da zhi wu ji su 。ji can yu diao kong zhi wu sheng chang fa yo de hu duo guo cheng ,tong shi zai zhi wu xiang ying sheng wu he fei sheng wu xie pai zhong fa hui zuo yong 。shui dao (Oryza sativa)shi shi jie shang zui chong yao de liang shi zuo wu zhi yi ,ji chan liang he zhi liang guan hu jue da bu fen ren kou de sheng cun ,er he fei shi (Nilaparvata lugens St?l,BPH)zuo wei shui dao chan shi xing hai chong shi shui dao sheng chan zhong chong yao de hai chong zhi yi 。ben wen cong yi ge you cai su nei zhi xin hao tong lu xia you de zhuai lu yin zi OsDLT(DWARF AND LOW-TILLERING)chu fa ,yan jiu ji diao kong de shui dao kang chong fang yu xin hao wang lao ,jie guo ru xia :(1)xiang bi yu xiang tong fa yo jie duan de ye sheng xing shui dao ,tu bian ti shui dao dltdui he fei shi de qu shi wei hai ju you geng jiang de nai shou xing 。tong shi ,bu tong fa yo jie duan de ye sheng xing shui dao he 48ri ling dltshui dao de he fei shi shua ze xing shi yan biao ming he fei shi geng xi ai wei hai ge ge fa yo jie duan de ye sheng xing shui dao 。(2)OsDLTji yin guo liang biao da zeng jia le shui dao dui he fei shi de min gan xing ,yu ye sheng xing shui dao xiang bi he fei shi ci cheng chong geng xi huan zai guo liang biao da OsDLTqu shi he chan luan 。qu shi guo liang biao da OsDLTshui dao de chu yu hua ci cheng chong fen bi de mi lou liang xian zhe zeng jia ;he fei shi re chong fa yo li ji xian zhe su duan ,F1dai chan ci chan luan liang he luan fu hua lv xian zhe shang sheng 。(3)yu wei chu li 9311xiang bi ,he fei shi ci cheng chong geng qu xiang yu zai 24-biao you cai su nei zhi (24-epicastasterone,ECS)chu li de 9311shui dao shang qu shi he chan luan ;er he fei shi ci cheng chong dui wei chu li he ECSchu li de OsDLTgong neng que shi tu bian ti shui dao dltzhi jian mo qu shi he chan luan pian hao 。bing huan zuo (Propiconazole,PCZ)shi zhi wu BRge cheng yi zhi ji ,yu wei chu li 9311xiang bi he fei shi ci cheng chong zai PCZchu li 9311shui dao shang de qu shi he chan luan liang xian zhe jian shao ;er yu wei chu li de dlttu bian ti shui dao xiang bi ,he fei shi ci cheng chong zai PCZchu li de dltshui dao shang de qu shi he chan luan liang xian zhe zeng jia 。yu wei chu li de 9311shui dao xiang bi ,jing Bikininchu li de 9311shui dao dui he fei shi ci cheng chong qu shi he chan luan de min gan xing xian zhe de jiang di ;er dlttu bian ti ,Bikininchu li zeng jia he fei shi ci cheng chong qu shi he chan luan min gan xing 。(4)zhuai lu zu shu ju fen xi hou de dao 7196ge cha yi biao da ji yin 。dui he fei shi qu shi chu li 9311shui dao (WB)he dltshui dao (dB)chu xian de cha yi biao da ji yin jin hang fen xi ,mo li suan tu jing zhong zong gong you 18ge ji yin chu xian le shang diao ,zhan gai tu jing cha yi biao da ji yin de 81.83%。shui yang suan tu jing zhong zong gong you 15ge ji yin chu xian cha yi biao da ,ji zhong 11ge ji yin chu xian shang diao zhan shui yang suan tu jing cha yi biao da ji yin de 73.33%。18ge fang yu xiang guan de WRKYjia zu zhan WRKYjia zu cha yi biao da ji yin de 85.71%,9ge ERFjia zu cha yi biao da ji yin quan bu shang diao 。3ge bing cheng xiang guan dan bai dan bai 、5ge ji ding zhi mei chu xian le xian zhe shang diao 。(5)tong guo gou jian jiao mu shuang za jiao ti ji zheng ming le OsDLTdan bai zi shen bu ju you zi ji huo bing shai shua chu 3ge he OsDLTdan bai ju you xiang hu zuo yong de dan bai bian ma ji yin 。zhi hou ke long bing gou jian le 4ge mo li suan yi zhi zi JAZjia zu ji yin de ji huo zhi li ,tong guo jiao mu shuang za jiao zheng ming OsJAZ6yu OsDLTdan bai ke yi chan sheng xiang hu zuo yong 。ben wen yan jiu biao ming ,OsDLTzai he fei shi you dao de fang yu fan ying zhong fa hui chong yao zuo yong ,gai ji yin fu diao kong shui dao dui he fei shi de kang xing ;OsDLTke neng shi you cai su nei zhi tu jing yu mo li suan tu jing hu zuo de chong yao yin zi ,zai diao kong shui dao de kang chong fang yu fan ying 。
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论文详细介绍
论文作者分别是来自浙江农林大学的王喆，发表于刊物浙江农林大学2019-06-03论文，是一篇关于水稻论文,油菜素内酯论文,褐飞虱论文,诱导防御论文,浙江农林大学2019-06-03论文的文章。本文可供学术参考使用，各位学者可以免费参考阅读下载，文章观点不代表本站观点，资料来自浙江农林大学2019-06-03论文网站，若本站收录的文献无意侵犯了您的著作版权，请联系我们删除。
标签：水稻论文; 油菜素内酯论文; 褐飞虱论文; 诱导防御论文; 浙江农林大学2019-06-03论文;